小儿慢性乙型肝炎患者外周血Th17细胞／调节性T细胞失衡的研究

摘要

Objective To study the frequency of peripheral blood T helper 17(Th17)and regulatory T cells(Treg)and their as-sociated cytokines level in child chronic hepatitis B(CHB)and to investigate the balance characteristics of Th1 7/Treg under differ-ent progression status in child CHB.Methods Peripheral blood and serum of 60 children with CHB and 16 healthy controls were collected for detecting the frequency of peripheral blood Fox3+Treg and Th1 7 by the flow cytometry and serum levels of Th1 7/Treg differentiation associated cytokine IL-17A,TGF-β,IL-22,IL-23,IL-6 and IL-1βwere detected by the enzyme-linked immu-nosorbent assay(ELISA).Results The frequency of peripheral blood Th1 7 and Treg,and the ratio of Th1 7/Treg in the CHB group were significantly higher than those in the healthy controls,the frequency of Th1 7 was positively correlated with disease progres-sion.In addition,serum IL-17A,IL-22 and IL-23 levels in the CHB children were higher than those in the healthy controls(P<0. 05).Conclusion Th17 and Treg play the important role in the mechanism of child CHB.The imbalance of Th17/Treg exists in child CHB.%目的：探讨小儿慢性乙型肝炎（CHB）患者不同疾病进展状态下Th17细胞/调节性 T细胞（Treg细胞）的平衡特点。方法入组16例健康对照（对照组）、60例小儿CHB患者（CHB组），采集外周血及血清，采用流式细胞仪检测 Fox3＋Treg细胞及Th17细胞的频率，并用酶联免疫吸附试验方法（ELISA）检测血清中 Th17/Treg分化相关细胞因子。结果 CHB组的 Treg细胞、Th17细胞频率及Th17/Treg比值显著高于对照组，Th17频率与疾病进展呈正相关。CHB组患者血清中 IL-17A、IL-22及IL-23水平高于对照组（P＜0．05）。结论小儿CHB组患者存在Th17/Treg的失衡。