HPLC法同时测定养心定悸胶囊中4种成分

摘要

AIM To establish an HPLC method for the simultaneous content determination of four constituents in Yangxin Dingji Capsules (a cardiac tonic for palpitation,containing Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Cinnamomi Ramulus,Rehmanniae Radix,etc.).METHODS The analysis of 50％ methanol extract of Yangxin Dingji Capsules was performed on a 40 ℃ thermostatic Diamonsil C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 265 nm.RESULTS Liquiritin,glycyrrhizic acid,cinnamic acid and cinnamic aldehyde showed good linear relationships within the ranges of 1.00-80.24 μg/mL (r=0.999 0),2.52-100.70 μg/mL (r--0.999 7),0.50-40.40 μg/mL (r =1.000 0) and 0.66-52.96 μg/mL (r =1.000 0),whose average recoveries were 97.74％,100.97％,101.48％ and 99.49％ with the RSDs of 0.45％,1.11％,1.27％ and 1.66％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Yangxin Dingji Capsules.%目的 建立HPLC法同时测定养心定悸胶囊(炙甘草、桂枝、地黄等)中4种成分的含有量.方法 养心定悸胶囊50％甲醇提取液的分析采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm);以0.1％甲酸-乙腈为流动相,梯度洗脱;体积流量1 mL/min;柱温40℃;检测波长265 nm.结果 甘草苷、甘草酸、桂皮酸、桂皮醛分别在1.00～80.24 μg/mL(r=0.999 0)、2.52～100.70 μg/mL(r=0.999 7)、0.50 ～40.40 μg/mL (r=1.000 0)、0.66～52.96 μg/mL(r=1.000 0)范围内线性关系良好,平均加样回收率分别为97.74％、100.97％、101.48％、99.49％,RSD分别为0.45％、1.11％、1.27％、1.66％.结论 该方法简便准确,重复性好,可用于养心定悸胶囊的质量控制.