A method was established for simultaneously determination of diclazuril, toltrazuril, toltrazuril sulphone and toltrazuil sulphoxide in chicken muscle by HPLC. The analytes were extracted with acetonitrile, defatted with n-hexane, and then clean up by alumina solid-phase extraction cartridges. The eluent was evaporated by nitrogen flow to dryness and dissolved the residues with mobile phase. HPLC was employed for separation and UV detector was used for detecting directly. Qualitative analysis was done by retention time and quantitative analysis by peak area respectively. The results showed that good linear relationship were obtained in the range of 0.05~5 μg/mL, with the correlation coefficients were all above 0. 999. The average recoveries ranged from 70% to 110% of the spiked level at 50~500μg/kg, and the intra-assay coefficient and the inter-assay coefficient of variation were all below 15%. The method was simple, sensitive and stable.%建立了一种高效液相色谱法同时测定鸡肌肉中地克珠利、妥曲珠利、妥曲珠利砜及妥曲珠利亚砜残留量的方法。试样经乙腈涡旋提取，正己烷脱脂，经碱性氧化铝小柱净化，浓缩，流动相定容，高效液相色谱分离，紫外检测器检测，以保留时间定性，以峰面积定量。结果表明：该方法在0．05～5μg／mL浓度范围内线性关系良好，相关系数r均大于0．999。在50～500μg／kg添加浓度范围内，方法回收率在70％～110％之间，批内和批间变异系数均小于15％。方法简便、灵敏度高、稳定性好。
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