广藿香油对家兔内毒素性发热的作用

摘要

目的：探讨广藿香油对家兔内毒素性发热的解热作用及机制。方法：根据实验当天体重及基础体温将42雄性只家兔随机分为7组，分别为空白对照组、模型对照组、西药阳性组、中药阳性组及广藿香油高、中、低（2％、1％和0．5％）3个剂量组。除空白对照组外，其余6组均按1 mL/kg经耳缘静脉注射大肠杆菌内毒素（2 mg/L）复制家兔内毒素性发热模型，0．5 h后经耳缘静脉给药，空白和模型对照组注射等量的0．5％吐温80，西药、中药阳性组和广藿香油高、中、低剂量组分别注射对应剂量药物，观察各组家兔体温变化，采用放射免疫法测定血清中白细胞介素-1β（ IL-1β）和肿瘤坏死因子α（ TNF-α）以及下丘脑环磷酸腺苷（ cAMP）和前列腺素E2（ PGE2）的含量。结果：与空白对照组相比，模型对照组家兔体温、IL-1β、TNF-α、cAMP和PGE2显著上升（ P＜0．01或P＜0.05）；广藿香油对发热家兔的体温升高有明显的抑制作用，广藿香油高剂量组给药后1.5～5.5 h，家兔体温分别升高（1.06±1.55）、（1.62±1.36）、（1.38±1.22）、（0.98±0.98）和（0.48±0.95）℃，广藿香油中剂量组给药后3.5～5.5 h，体温分别升高（1.47±0.73）、（1.15±0.68）和（0.63±0.54）℃，广藿香油低剂量组给药后各时点均有降低发热家兔体温的趋势；广藿香油可明显抑制血清中TNF-α和下丘脑中cAMP含量的升高，有降低血清中IL-1β和下丘脑中PGE2含量升高的趋势。结论：广藿香油对家兔内毒性发热有显著解热作用，其机制可能与抑制血清中TNF-α和下丘脑中cAMP含量升高有关。%[ ABSTRACT] AIM:To investigate the antipyretic effect of patchouli oil on lipopolysaccharide ( LPS)-induced fe-ver in rabbits.METHODS:Male rabbits (n=42) were randomly divided into 7 groups according to their body weight and basal body temperature, including control group, model group, western medical positive group, traditional Chinese medical positive group, and high, middle and low doses (2%, 1%and 0.5%) of patchouli oil groups.Subsequently, except the controls, the rabbits were injected with LPS at a dose of 1 mL/kg (2 mg/L) through marginal ear vein to establish rabbit fever model and the rabbits in control group received the same volume of NS.The rabbits in control group and model group were injected with 0.5%Tween-80 0.5 h late, and the rabbits in the other groups were treated with correspoonding drugs. The effect of patchouli oil on the body temperature was observed, and the levels of interleukin-1β( IL-1β) and tumor nec-rosis factor-α(TNF-α) in the serum, and prostaglandin E2(PGE2) and cyclic adenosine monophosphate (cAMP) in the hypothalamus were measured by radioimmunoassay.RESULTS: The body temperature and the levels of IL-1β, TNF-α, cAMP and PGE2 in model group were significant higher than those in control group.Patchouli oil notably inhibited the body temperature in the febrile rabbits.From 1.5 h to 5.5 h after administration, the body temperatures were increased by (1.06 ±1.55), (1.62 ±1.36), (1.38 ±1.22), (0.98 ±0.98) and (0.48 ±0.95) ℃in high patchouli oil group, re-spectively.From 3.5 to 5.5 h after administration, the body temperatures were elevated by ( 1.47 ±0.73 ) , ( 1.15 ± 0.68) and (0.63 ±0.54) ℃ in middle patchouli oil group, respectively.A tendency of downregulation of the elevated body temperatures was observed at every time point after administration in low patchouli oil group.Patchouli oil significantly decreased the levels of TNF-αin the serum and cAMP content in the hypothalamus, and attenuated the elevated tendency of the IL-1βlevel in the serum and PGE2 level in the hypothalamus.CONCLUSION:Patchouli oil evidently has antipyretic effect on LPS-induced fever in the rabbits.The antipyretic mechanism might be related to the inhibition of TNF-αlevel in serum and cAMP content in the hypothalamus.