目的 探讨Cdk5的激活剂-p39mRNA在脑发育过程中的不同表达.方法 用Northern blot法和原位杂交法检测p39mRNA在大鼠脑发育过程中的表达.结果 Northern blot结果 显示,在15 d胚胎和新生大鼠脑中,p39mRNA表达较低;出生后1～3周表达最显著;到成年大鼠,表达降低到与新生大鼠脑相同的水平.原位杂交法显示,p39mRNA在新生大鼠脑中所有区域神经元中表达较低;而在3周龄大鼠脑的所有区域表达最高;到成年大鼠,大多数神经元中表达都降低,仅小脑还保持着高水平的表达.结论大鼠脑发育过程中,p39 mRNA在空间上和时间上有不同的表达,其亚细胞定位可能与激活Cdk5功能有关.%Objective To detect the different distribution of Cdk5 two activators, p35 and p39, throughout brain development. Methods We examined the spatial and temporal expression of p39 in the developing rat brains by Northern blot analysis and in situ hybridization. Results Northern blot analysis showed that p39 expression was low in 15-day old fetuses and newborns, and was most prominent in the 1-3 week-old rat brains. In the adult rat brains, expression declined to the same level as in newborn rat brains. In situ hybridization showed that p39 mRNA was weakly expressed in all neurons of all regions in the newborn rat brains and the transcriptional level was highest in all regions in the 3 week-old rat brains. In the adult rat brains, the expression was decreased in most neurons, except Purkinje and granule cells in the cerebellum which retained high levels. Conclusion The expression of p39 varies spatially and temporally along with rat brain development, and its subcellular location is related with the activation of Cdk5 function.
展开▼
