21例侵袭性肺曲霉病患者Th以及Treg细胞的检测

摘要

Objective To analysis CD4+T cells in peripheral blood mononuclear cell and clinical data of invasive pulmonary as-pergillosis( IPA)patients and explore function of cellular immunity mediated by helper T cells and regulatory T cells in IPA Meth-ods A total of 21 IPA patients were evaluated 19 healthy volunteers as the normal control group We detected Th1,Th2,Th17 and Treg expression in CD4+T cells,related cytokine secretion and key transcription factors of IPA patients and controls Results Pa-tients with IPA exhibited decreased Th1 and Treg expression,related cytokines( IFN-γ,IL-17A,TGF-β)and T-bet,RORγt and Foxp3 mRNA levels when compared with the normal control group while there was no significant difference between patients with IPA and the normal control group about peripheral Th2 frequency,Th2 related cytokines IL-4,and GATA-3 mRNA levels Conclu-sions Th1,Th17and Treg-mediate cellular immunity were inhibited in IPA patients.%目的：分析侵袭性肺曲霉病患者辅助性T细胞（ Th）以及调节性T细胞（ Treg）在外周血中单个核细胞中的表达情况及其临床相关性，探讨Th和Treg细胞介导的免疫反应在侵袭性肺曲霉病中的作用。方法分离21例侵袭性肺曲霉病患者及19例健康人外周血的单个核细胞，采用流式细胞术分析Th1、Th2、Th17、Treg细胞群的表达情况，Real-time PCR方法检测相关转录因子T-bet、GATA-3、RORγt以及Foxp3的表达，ELISA法检测血清中相关细胞因子IFN-γ、IL-4、IL-17以及TGF-β的表达。结果与健康人对照组相比，侵袭性肺曲霉病患者Th1细胞以及Treg细胞占CD4+T细胞的比例较之对照组明显降低；Th1、Th17、Treg细胞相关转录因子T-bet、RORγt、Foxp3以及相关细胞因子IFN-γ、IL-17A、TGF-β与对照组相比表达明显降低。结论 IPA患者的Th1、Th17以及Treg细胞所介导的免疫反应受抑制。