白假丝酵母菌突变株 ORF19．2500的功能研究

摘要

目的：筛选50株白假丝酵母菌基因缺失菌，寻找出对白假丝酵母菌生物被膜形成相关基因，进一步探究白假丝酵母菌生物膜的致病机制。方法利用培养生物被膜的方法筛选50株基因缺失菌；利用 XTT 法验证所筛选出的白假丝酵母菌突变株 ORF19．2500生物被膜形成缺陷；进一步观察 ORF19．2500基因缺失菌生长、菌丝形成。结果用 XTT 法证明白假丝酵母菌突变株 orf19．2500生物膜形成缺陷，且生长曲线和滴琼脂平板的方法均提示其生长速率减慢。在 spider 培养基上白假丝酵母菌突变株 orf19．2500不能诱导菌丝形成，但在 YPD＋10％小牛血清则菌丝形成正常。结论白假丝酵母菌突变株 orf19．2500可利用 spider 培养基缺陷而影响其酵母、菌丝二态性的转化，及其生物被膜的形成。%Objective For searching some genes which impacted the formation of Candida albicans biofilm.Then we screened of 50 Candida albicans mutants.So as to identify potential pathogen mechanism of Candida albicans.Methods Screening 50 Candida albicans mutants in the method of cultivating biofilm in vivo.Biofilm was quantified by XTT.Then the mutants of growth rate and hy⁃phal formation were analyzed.Results It was defected for Candida albicans mutant orf19.2500 to form biofilm which was quantified by XTT.The mutant grows slowly,which was quantified by growth curve and spot assay.Then we find the mutant can not form hyphal in liquid spider media,while it can normally form hyphal in liquid YPD plus 10% fetal bovine serum media.Conclusion The Candi⁃da albicans mutant orf19.2500 can not utilize liquid media which impacted morphogenetic switching and the biofilm formation.