骨形成蛋白-2和成纤维细胞生长因子对人牙周膜细胞增殖的联合效应

摘要

目的：探讨重组人骨形成蛋白-2（rhBMP-2）和碱性成纤维细胞生长因子(bFGF)单独或联合作用对人牙周膜细胞（PDLCs）增殖的影响。方法：体外培养人PDLCs，分别用不同浓度的rhBMP-2和bFGF单独或联合作用，用四唑盐比色法（MTT法）进行观察。结果：rhBMP-2和bFGF单独作用后PDLCs的增殖较对照组有明显的升高；而rhBMP-2与bFGF联合作用后PDLCs的增殖较各自单独作用有更明显的升高（P<0.05）。结论：rhBMP-2与bFGF联合应用对于促进人PDLCs的增殖具有协同作用。%AIM: To evaluate the effects of a combination of recombinanthuman bone morphogenetic protein-2 (rhBMP-2)and basic fibroblast growth factor(bFGF) on the proliferation of periodontal ligament cells(PDLCs). METHODS: Using cell culture technique, enzyme kinetics methods. RESULTS: Both rhBMP-2 and bFGF could promote the proliferation of PDLCs. Significantly greater proliferation was found in combined group comparing with individual groups(P<0.05). CONCLUSION: rhBMP-2 and bFGF could promote the proliferation of PDLCs and had synergistic action.