目的:研究基质细胞衍生因子1(Stromal cell-derived factor-1,SDF-1)及其受体CXCR4在人炎性牙髓组织中的表达,探讨SDF-1/CXCR4轴在牙髓炎症发生发展中的可能作用.方法:采用免疫组织化学染色的方法检测SDF-1和CXCR4阳性细胞在健康、炎症牙髓组织中的分布情况.以实时荧光定量RT-PCR方法检测SDF-1mRNA在健康和炎症牙髓中的表达.结果:炎性牙髓中SDF-1、CXCR4主要分布于炎性细胞、成牙本质细胞和微血管内皮细胞.而正常组牙髓少见SDF-1、CXCR4阳性细胞.炎性牙髓中SDF-1mRNA的表达较健康牙髓显著增强.结论:与正常牙髓相比,炎性牙髓组织中SDF-1、CXCR4阳性细胞明显增多.炎性牙髓中SDF-1表达水平明显上调.SDF-1/CXCR4轴可能参与了牙髓炎症损伤和修复过程.%AIM: To investigate the expression of Stromal cell-derived factor-1 (SDF-1) and CXCR4 inhuman inilammed dental pulps and to explore the role of SDF-1/CXCR4 axis in pulpal inflammation. METHODS: Inflamed dental pulp tissues were obtained from extracted teeth that showed spontaneous pain and/or lingering pain in response to cold and/or heat stimulus, and normal dental pulp tissues were obtained from healthy noncarious teeth. Distribution of SDF-1 -positive and CXCR4-positive cells in the dental pulp tissues was investigated by immunohistochem-istry. Real time RT-PCR was performed to detect SDF-1 Mrna expression in healthy and inflamed pulp tissues. RESULTS: In inflamed pulps, SDF-1 and CXCR4 were mostly distributed in inflammatory cells, odontoblasts and micro-vascular endothelial cells. In contrast, SDF-1 and CXCR4 were scarcely detected in normal pulp tissues. SDF-1 Mrna expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. CONCLUSION: SDF-1-positive and CXCR4-positive cells were significantly increased in inflamed dental pulp tissues as compared with non-inflamed dental pulp tissues. Our findings suggest that SDF-1 plays an important role in the advancement of pulpal inflammation via the recruitment of CXCR4-expressing inflammatory cell and SDF-1/CXCR4 axis maybe involved in the recruitment of dental pulp inflammatory cells at the injury site.
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