Piezos离子通道在大鼠牙周组织中表达的实验研究

摘要

目的：通过观察机械敏感离子通道Piezos蛋白在牙周组织中的表达情况，探讨其在牙周组织机械感受中的作用。方法：使用间接免疫荧光技术，在蛋白水平观察机械敏感离子通道Piezos蛋白在大鼠牙周组织的表达特点及定位。结果：Piezo1和Piezo2在牙周膜成纤维细胞中均呈阳性表达，其中Piezo2的表达量明显低于Piezo1（P＜0．05）；Piezos两种蛋白主要表达于牙周膜成纤维细胞，但在骨细胞中未见表达；Piezos在细胞中的表达部位主要在胞膜上，部分在胞浆里。结论：牙周组织，特别是牙周膜中有丰富的Piezos离子通道的表达，提示Piezos可能参与了牙周膜的感觉传导。%AIM:To observe Piezo protein expression in periodontal tissues.METHODS:Piezo expression in periodontal tissues of rats was detected by indirect immunofluorescence technique.RESULTS:Positive staining of Piezo1 and Piezo2 was observed in periodontal fibroblasts of rats while the expression of Piezo2 was obviously lower than that of Piezo1 (P<0.05).Piezos were mainly expressed in periodontal ligament fibroblasts,but no positive staining was found in osteocytes.Piezos’expression in the plasma membrane of periodontal ligament fibroblasts was higher than that in the cytoplasm.CONCLUSION:There is a rich expression of Piezos ion channel in periodontal ligament.Pi-ezos play a role in perceptive function of periodontal ligament.