PPAR-γ和PTEN蛋白在膀胱移行细胞癌中的表达及意义

摘要

目的 测定膀胱移行细胞癌中PPAR-γ和PTEN蛋白的表达及与其病理分级、临床分期及预后相关性.方法 免疫组织化学法检测PPAR-γ和PTEN于膀胱移行细胞癌标本及正常膀胱组织中的表达程度,病理分析仪测定蛋白表达程度.结果 ①在正常膀胱组织中PPAR-γ蛋白的表达(20％)明显低于膀胱癌(68％),而正常膀胱组织PTEN蛋白的表达(100％)明显高于膀胱癌(52％),两者阳性率差异有统计学意义(P＜0.05).②膀胱移行细胞癌组织中PPAR-γ表达与临床分期、病理分期、淋巴结转移呈正相关性,PTEN蛋白表达呈负相关性.③PPAR-γ蛋白和PTEN蛋白在膀胱移行细胞癌中表达呈负相关关系(r=-0.604,P＜0.05).结论 PPAR-γ和PTEN可能参与了膀胱肿瘤的发生发展过程,对膀胱癌诊断、临床分期及预后有一定价值.%Objective To exolore the expression of ppar-gamma and PTEN in cell carcinoma of the bladder and the its correlation with pathologic grade,clinical stage and prognosis.Methods The expression of ppar-gamma and PTEN in bladder transitional cell carcinoma specimens and normal bladder tissues was detected by immunohistochemistry,and the expression level of protein was detected by pathological analyzer.Results ① The expression of PPAR-gamma protein (20％) in normal bladder tissues was significantly lower than that of bladder cancer(68 ％),while the expression of PTEN protein in normal bladder tissues (100％)was significantly higher than that in bladder cancer(52％).The positive rate of the two markers had significant difference (P ＜ 0.01) . ② The expression of PPAR-gamma was positively correlated with clinical stage,pathological stage and lymph node metastasis in bladder transitional cell carcinoma,and negatively correlated with PTEN protein expression.③There was a negative correlation between PPAR-gamma protein and PTEN protein in bladder transitional cell carcinoma (r =-0.604,P ＜ 0.05).Conclusion Ppar-gamma and PTEN may be involved the occurrence and development of bladder cancer and maybe has some value in clinical diagnosis,staging and prognosis judgment of bladder cancer.