目的 选择最优的载体包埋固定化AEM0957细胞酶促催化脱氧胸苷和腺嘌呤合成脱氧腺苷.方法 分别用琼脂、聚丙烯酰胺、明胶、卡拉胶、海藻酸钠、聚乙烯醇等6种材料包埋固定细菌AEM0957细胞,以此为催化剂转化脱氧胸苷合成脱氧腺苷.结果 通过优化反应条件,聚丙烯酰胺固定化细胞催化脱氧胸苷的转化率可达57％,高于游离细胞反应能力,并能多次重复使用,10次重复反应的转化率仍能够保持在32％左右.结论 聚丙烯酰胺在稳定性、转化性能等方面优于其它材料,该研究为脱氧腺苷的工业化生产奠定了重要基础.%Purpose Choose the best carrier of immobilized AEM0957 cell for the enzymatic synthesis of deoxyadenosine from thymidine and adenine. Methods Agar, polyacrylamide, gelatin, carrageenan, sodium alginate and polyvinyl alcohol were used as immobilization materials to encapsulate bacteria AEM0957 cells, and these immobilized cells were used as catalyst to synthesize 2'-deoxyadenosine from 2'-deoxythymidine. Results By optimizing the reaction conditions, the conversion rat of deoxythymidine catalyzed by immlbilized cells with polyacrylamide achieved 57% , which was higher than that of by free cells. After 9 batches of repeated reaction, the conversion rate was remained at about 32% . Conclusion Polyacrylamide was better than other materials in stability and catalytic efficiency. The study laid an important foundation for the industrial production of deoxyadenosine.
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