USING CYTOGENETIC ANALYSIS OF MOUSE EGGS FERTILIZED IN VITRO FOR MONITORING THE MUTAGENICITY OF CHEMICALS ACTING ON GERM CELLS——1.Method for preparation of slides of chromosome of mouse eggs at the first cleavage metaphase and the preliminary use

摘要

In this experiment,the sperms were obtained from(C57×CBA) F1 male mice and the eggs from NIHstrain female mice.Using the fertilization techni-que in Vitro,the slides of chromosome of mouse eggsat the first cleavage metaphase were prepared.