TERT过表达对人骨髓间充质干细胞精氨酸组蛋白甲基化酶表达影响的研究

摘要

目的 探索端粒酶对间充质干细胞精氨酸组蛋白甲基化酶和组蛋白甲基化状态的影响.方法 利用逆转录病毒将端粒酶催化亚基转染到人骨髓间充质干细胞建立稳定转染细胞系,研究TERT过表达后对精氨酸组蛋白甲基化酶基因族的主要相关基因表达的影响.结果 人骨髓间充质干细胞转染端粒酶催化亚基后,精氨酸组蛋白甲基酶基因超家族中的9个基因PRMT1~9的表达明显下降,组蛋白甲基化水平整体降低.结论 TERT过表达抑制了骨髓间充质干细胞精氨酸组蛋白甲基酶的表达,从而可能抑制精氨酸组蛋白的甲基化,精氨酸组蛋白甲基化水平降低有利于间充质干细胞维持年轻的状态.%Objective To examine the effect of Lelomerase on the expression of arginine hislone melhylase in human bone marrow mesenchymal stem cells ( hBMSCs) . Methods Relroviral human Lelomerase reverse transcriplase (hTERT) was transfecled into human bone marrow mesenchymal slem cells Lo establish slably transfecled cell lines. The effecl of TERT overexpression on the relaled gene expression of arginine hislone melhylase gene family was examined. Results After hBMSCs were lransfecled with the lelomerase catalytic subunil, the expression of the genes PRMT1-9 in the arginine hislone melhyllransferase gene superfamily was down-regulated, and the overall level of hislone melhylalion decreased. Conclusion TERT overexpression inhibits the expression of hBMSCs in arginine hislone demelhylase, which may inhibit the melhylalion of arginine hislone.