BMP4对牙源性干细胞精氨酸组蛋白甲基化酶表达影响的研究

摘要

Objective To examine the effect of human recombinant bone morphogenetic protein 4 (BMP4) on the expression of arginine histone methylase in dental tissue derived mesenchymal stem cells .Methods BMP4 was used to induce the stem cells from apical papilla ( SCAPs ) and periodontal ligament stem cells ( PDLSCs ) .The related gene expression of arginine histone methylase , including PRMT1-9, was examined by real-time PCR.Results After the induction of 50 ng/ml BMP4, the expression of PRMT2, PRMT4, PRMT5, PRMT6, PRMT7, and PRMT9 were up-regulated in SCAPs, while only PRMT6 expression was increased in PDLSCs after 50 ng/ml BMP4 treatment.Conclusion BMP4 promoted the expression of some arginine histone methylase in SCAPs and PDLSCs , which may play a role in the osteogenic differentiation of dental tissue derived MSCs .%目的 探索人重组骨形成蛋白4(BMP4)对牙源性干细胞精氨酸组蛋白甲基化酶表达的影响.方法 利用BMP4对根尖牙乳头干细胞和牙周膜干细胞进行诱导,Real-time PCR检测精氨酸组蛋白甲基化酶基因家族的主要相关基因PMRT1～9的表达变化.结果 Real-time PCR结果显示50 ng/ml BMP4促进根尖牙乳头干细胞精氨酸组蛋白甲基化酶基因家族中的PRMT2、PRMT4、PRMT5、PRMT6、PRMT7和PRMT9的表达,而在牙周膜干细胞50 ng/ml BMP4只促进PRMT6的表达.结论 BMP4促进根尖牙乳头干细胞和牙周膜干细胞部分精氨酸组蛋白甲基化酶的表达,可能在牙源性干细胞成骨分化中起一定的作用.