ALDH1A3和CD133在皮肤鳞状细胞癌中的表达及其临床意义

摘要

Objective:To investigate the expression and clinical significance of tumor stem cell markers ALDH1A3 and CD133 in squamous-cell carcinoma of skin.Methods:The tissue samples were obtained from 34 patients of cutaneous squamous-cell cancer and 11 subjects undergone plastic surgery for re-moval of the normal skins in our department between 2012 and 2016. Immunohistochemical method was used to detect the expression of ALDH1A3 and CD133 protein in the tissues of the two groups.Results:ALDH1A3 and CD133 were highly expressed in cutaneous squamous-cell carcinoma,yet moderately expressed in the tissues of normal skins[positive rate:73.52%(24/34) and 79. 41%(27/34] vs.18. 18%(2/11) and 9.09%)(1/11)].Although both ALDH1A3 and CD133 were expressed in the tissues of cutaneous squamous-cell carcinoma and normal skins,yet the expression was higher in the tumor tissues,with significant difference(P<0.05).Conclusion:Abnormal expression of ALDH1A3 and CD133 protein was seen in cutaneous squamous-cell cancer,suggesting that the two proteins can be important roles in the occurrence,development and staging of squamous cell carcinoma of the skin,and may serve as a marker for clinical diagnosis of this neoplasm.%目的:探讨和研究肿瘤干细胞标志物ALDH1A3和CD133在人皮肤鳞状细胞癌中的表达及其临床意义.方法:收集我院烧伤整形外科2012~2016年手术切除34例皮肤鳞癌组织蜡块标本及11例整形手术切除正常皮肤组织,采用免疫组化方法对两组标本中的ALDH1A3和CD133蛋白表达水平进行检测.结果:ALDH1A3和CD133在皮肤鳞状细胞癌患者瘤体组织中高表达(表达肿瘤干细胞标志蛋白的细胞比例较高),ALDH1A3阳性表达率为73.52%(25/34),CD133阳性表达率为79.41%(27/34).ALDH1A3和CD133在正常皮肤组织中低表达(表达肿瘤干细胞标志蛋白的细胞比例较低),ALDH1A3阳性表达率为18.18%(2/11),CD133阳性表达率为9.09%(1/11).ALDH1A3和CD133在皮肤鳞状细胞癌瘤体组织和正常皮肤组织均有表达,但在瘤体组织中的表达率更高,且两组差异性具有统计学意义(P<0.05).结论:ALDH1A3和CD133蛋白在皮肤鳞状细胞癌瘤体组织中呈异常高表达,可能在皮肤鳞状细胞癌的发生、发展、分期中起到重要作用,并将有可能作为皮肤鳞状细胞癌肿瘤干细胞新的标志物.