Real-time, Broad-Band Measurement of Cholesterol, Collagen and Elastin Using a Novel Rotary Switch Spectrometer

摘要

The present paper introduces an integrated sensing and processing spectroscopic system that combines Multivariate Factor Analysis (MFA) and Molecular Factor Computation (MFC) with a broad band light source, incorporating a rotary, multichannel optical device to produce real-time, spectroscopic analysis. The system is able to distinguish samples of cholesterol and collagen/elastin within a time range of 25 ms, by using near-infrared reflectance Spectroscopy. The potential for the use of the device for in-vivo coronary angiography will be discussed. The system presents several key features that make it unique as a spectroscopic tool: it is compact and rugged with multilayer interference wavelength selection, and capable of operation in high vibration environments. The system is also fast, with the capacity to reduce the measurement delay even further; it is broad band, with a bandwidth limited by the optical source and the sensitivity of the photodetector used; it will allow quantitative, simultaneous, multispecies detection; and it is amenable to different optical delivery/detection schemes. Standard telecommunications-grade parts comprise the system, bridging the gap between the prototype and manufactured product; these include: optical fibers, electrically actuated optical switches, and free-space optics elements. A detailed description of the data analysis algorithm will be presented, with special emphasis on its use for multispecies detection.