THE ARSENIC-PHYTOREMEDIATION POTENTIAL OF GENETICALLY MODIFIED PSEUDOMONAS SPP

摘要

Gene constructions rendering recombinant bacteria resistant to arsenic and increasing their ability to dissolve soil phosphates and/or arsenates were created by cloning the ars operon and the gene of citrate synthase from a chromosome of the strain Pseudomonas aeruginosa PA01. Genetically modified variants of the strain Pseudomonas aureofaciens BS1393 have been constructed that are resistant to high concentrations of arsenic and dissolve poorly soluble phosphates and/or arsenates. The recombinant strains P. aureofaciens BS1393(pUCP22∷ ars RBC) and P. aureofaciens BS1393(pUC P22∷ gltA) exerted positive effects on the survival of sorghum (Sorghum saccharatum L.) and its ability to accumulate arsenic.