Study of malignant brain gliomas using optical coherence tomography and terahertz pulsed spectroscopy aimed on advanced intraoperative neurodiagnosis

摘要

The problem of complete resection of human brain glioma during neurosurgery is still one of the most challenging, since the existed diagnostic methods are plagued with limited sensitivity and specificity; they remain laborious, time-consuming and/or rather expensive. The present work includes the ex vivo study of malignant brain gliomas featuring different grades (according to the World Health Organization) by means of two methods, i.e. optical coherence tomography (OCT) and terahertz pulsed spectroscopy (TPS). Both OCT and TPS studies were done just after the tissue resection and included gelatin embedding of the samples for conservation of water content. The further histological examination using hematoxylin and eosin (H&E) stained microscopy approved the diagnosis. The results demonstrate the potential of TPS to differentiate intact and malignant tissues and the potential of OCT to differentiate low- and high-grade gliomas as well as intact tissue and low-grade gliomas. Thus, combination of these modalities seems to be rather prospective for the further development of the advanced intraoperative diagnostic tools.