The comparative analysis of two homologous genomic islands associated with the noncoding sequence in Enterobacter cloacae

摘要

The genomic islands (GIs) are usually the products of horizontal gene transfer (HGT) that is evolution pattern in prokaryote. Two homologous GIs (WSU1GI~(No) and KU01GI~(No)) containing the homologous integrase of Bacteriophage P2 were determined in Enterobacter cloacae through flanking sequence alignment of the homologous integrase. The homologous GIs were integrated into the noncoding sequence. Their common flanking sequence is 5'-AAGGCTCCCTCAGGAGC-3', and their integrases share 97% similarity. About two-thirds of the nucleotide sequences between WSU1GI~(No) and KU01GI~(No) are highly similar. The different regions between WSU1GINo and KU01GINo mainly include hypothetical gene, Phage-related tail gene, capsid gene, and baseplate assembly gene. In conclusion, the tandem arrangement of WSU1GI~(No) and KU01GI~(No) will be artificially constructed because of their similar structural characteristics, and phage coat protein assembly will also be analyzed.