Identification of a Degrading-Bacterium SG-2 and Preparing Complex Microbial Community for Degrading Chlorpyrifos

摘要

A bacterium capable of degrading chlorpyrifos named SG-2 was isolated from the corn soil in Shenyang Institute of Technology, Northeast China. The bacterium SG-2 was identified as Bacillus sp., according to its morphological, physiological characteristics and the phylogenetic analysis of 16S-rRNA. A compound bacterium agent compound of SG-2 and Cunninghamella about the ability of degrading chlorpyrifos was prepared in this study. Incubation tests showed that the initial concentration of chlorpyrifos in culture was 100mg L~(-1), incubation for 48h at 30°C, the degrading rate of chlorpyrifos of SG-2, Cunninghamella and the compound bacterium agent were 65.12%, 56.36% and 83.71%, respectively. Further study should be conducted to investigate the degradation pathways of chlorpyrifos by bacteria SG-2 and the compound bacterium agent and the optimal conditions of the degradation in the soil system.