Confocal Fluorescence Detection of Cell-based Assays Using a Digital Micro-mirror Device

摘要

We report the development of a confocal fluorescence imaging system based on a digital micro-mirror device (DMD). A DMD has an advantage of offering lateral sample scanning with programmable light modulation at an ultrahigh speed, while providing pinhole arrays on the source and/or detector side to enhance depth and lateral resolution for confocal detection. In this paper, we present a DMD-based fluorescence detection system for studying dynamics of multiple cells in vitro that are cultured in a microfluidic cell culture device. For feasibility, we tested the optical system using USAF target and studied imaging with fluorescence microbeads (Φ = 10 μm) as an equivalent to a cell.