Haplotype, allele frequencies and population data of 17 Y-chromosome STR loci DYS456, DYS3891,DYS390, DYS389II, DYS458, DYS19, DYS385a, DYS385b, DYS393, DYS391, DYS439, DYS635, DYS392,YGATAH4, DYS437, DYS438 and DYS448 were determined from 71 unrelated male individuals belong toYousafzai tribe living in Swat Valley. Statistical analysis was performed and as many as 45 haplotypes wereidentified and 41 of them were observed only once, accounting for 91% unique haplotypes. The most commonhaplotypes were observed seventeen times (23.9%). Y chromosomal haplotypes diversity for 17 Y STRs locusset ranged from 0.232 to 0.577 in Yousufzai population indicating haplotypes among unrelated males. TheYousafzai allele frequency database was then compared with other populations of the world.
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