pCR-DGGE analysis after Bioleaching Stimulation by Indigenous Microorganisms in Vineyards Soil and Copper Mining Waste

摘要

Bioleaching techniques can be used to remove copper ions from contaminated soils and recover polluted areas in situ or ex situ. Several treatments with HC1, H_2SO_4, and FeSO_4 were used to stimulation of bioleaching in two experimental conditions applied in vineyard soil and in waste copper mining. The bioleaching treatment using FeSO_4 and H_2SO_4 mixed solution had more effect on copper lead, and the second condition using controlled temperature at 30°C and no water addition to collect soil solution, was more efficient than the first condition using room temperature deionized water to extract soil solution. The treatment with FeSO_4 and H_2SO_4 - in the second condition, bioleached more than 1,100 mu g/kg of copper from vineyard soil in one collects. After the bioleaching period (115 days), both substrates were analyzed to evaluate the effect of treatments on soil microbial by denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA fragments. A high diversity of bacterial was found in the vineyard site as Rhodobacter sp., Silicibacter sp., Bacillus sp., Paracoccus sp., Pediococcus sp., Myxococcales sp., Clostridium sp., Thiomonas sp., Firmicutes sp., Caulobacter vibrioides, Serratia sp. and Actinomycetales; in the copper mining waste basically was found Shingomonas sp. specie in most of treatments; it also was found Paracoccus sp. and Enterobacter sp. in this soil sample.