Injury and stimulation of Porcine Aortic Endothelial cells (PAE) with nucleotides or EGF result in differential phosphorylation of epidermal growth factor receptor (EGFR) and affect recruitment of the downstream signaling protein Grb2

摘要

Previous work in our lab (VTR) show that EGFR is phosphorylated after injury and nucleotide stimulation, despite the absence of EGF in wound media immediately after injury [Boucher et al. 2007]. We have further proved that P2Y (GPCR) receptors and EGFR crosstalk after injury or nucleotide stimulation [Boucher et al. 2007]. Understanding the post-translational modification of EGFR and how it might affect the affinity of the binding proteins that mediate the downstream signaling cascade after injury is therefore of great interest.