PATERNO-BUCHI PHOTO-DERIVATIZATION PROTOCOL FOR MALDI-MS IMAGING OF CARBONCARBON DOUBLE BOND POSITIONAL ISOMERS OF PHOSPHO- AND GLYCOLIPIDS IN BIOLOGICAL TISSUES

摘要

Current MALDI-MS imaging (MALDI-MSI) techniques visualize the global distribution of phospho- and glycolipids in tissue sections, but are generally blind to the physiologically relevant carbon-carbon double bond (C=C) position. Here we present a fully MALDI-(2)-MSI compatible "on-tissue" Paterno-Buchi (PB) photo-derivatization protocol that enables mapping the distribution of C=C positional isomers of numerous phospho- and glycolipids in mouse brain.