To test the potential of microarray technology for the identification of genes associated with wheat flour quality, gene expression during wheat endosperm development was analyzed with cDNA chips carrying about 2600 clones. RNA from a bread-making variety and a noodle-making variety were used as target materials. When endosperm RNA samples of these varieties were compared at 10 and 15 days post-anthesis, many of the monitored genes were expressed at detectable levels. Although there were not big differences in the expression patterns of the two varieties, 47 clones displayed greater than 2-fold differences in signal intensities. We concluded our array system was useful to screen candidate genes associated with particular flour qualities.
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