Imaging of lymph flow in single microvessels in vivo

摘要

In this study parameters of lymph microcirculation are investigated. The microcirculation was studied on small intestine mesentery in norm nad during Dimethyl sulfoxide (DMSO) application. The direct measurement of lymph flow velcoity (parameter V) in individual microvessesl was based on the technique of light intravital videomicroscopy. The first spectral moments of Doppler signal, characterizing the mean velocities of lymph flow in microvessels (parameter M_1), were measured by speckle-interferometrical method. Simultaneously, diameters of lymph microvessels as well as parameters of phasic contractions and valve function of lymphatics were registered. The value of V was very changeable; the mean V was equal to 270+-24 mum/s. The M_1 was the varying characteristic of the lymph flow too. The temporal dynamic of M_1 was reflected alternating-translation motion of lymph flow. DMSO application during 15 min caused the construction in a majority of lymphatics and the phasic contractions. DMSO induced lymphostasis in 20