摘要：
[Aim] Southern rice black-streaked dwarf virus (SRBSDV) is a persistent virus transmitted by the whitebacked planthopper (WBPH),Sogatella furcifera,among rice plants.This research aims to investigate whether SRBSDV can induce the changes in the types and contents of volatiles from rice plants,and the possible relationship between the host orientation behavior of WBPH and the volatile changes.[Methods] The volatiles from SRBSDV-infected and uninfected plants were respectively collected by using the dynamic headspace,and analyzed on GC-MS.The behavioral responses of WBPH to the volatiles detected exclusively in the infected plants were tested by using olfactometer.[Results]Thirty-six volatile compounds belonging to 11 categories were detected from the uninfected plants.Thirtyseven compounds belonging to 12 categories were detected from the SRBSDV-infected plants.Twelve compounds,i.e.,n-hexadecane,didecyl ether,butyl octyl phthalate,n-octadecane,diisobutyl adipate (DA),pentadecanal,methyl hexadecanoate (MH),2-methylhexacosane,benzophenone,cedrol,neicosane and plant alcohol,were detected exclusively in the infected plants.Meanwhile,six compounds,i.e.,1-tricosene,octadecanal,naphthalene,perhydrofamesyl acetone,lauryl alcohol and butylated hydroxytoluene (BHT),were detected both in the infected and uninfected plants,but had significant differences in their contents.Olfactory selection experiments showed that only plant alcohol exhibited distinctly repellent activity against WBPH at the concentration of 50 μL/L.[Conclusion] The changes in the types and contents of rice volatiles are related to rice varieties and its developmental stages.Plant alcohol is only detected in the rice variety Rongyouhuazhan at the booting stage and has significantly repellent effects on WBPH.However,more research is needed to clarify whether plant alcohol can be helpful for the transmission of SRBSDV.%[目的]南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)是白背飞虱Sogatella furcifera在稻苗间传播的一种持久性病毒.本研究旨在调查SRBSDV是否诱导水稻挥发物的含量及成分发生变化,并探究变化的组分是否对白背飞虱具引诱或驱避作用.[方法]采用动态顶空法收集3个水稻品种病/健植株的挥发物,利用气相色谱-质谱联用仪(GC-MS)分析各品种不同生育期病/健株挥发物的组分,使用嗅觉测定仪测定了白背飞虱对病株特有挥发物的嗅觉行为反应.[结果]从健株检测到11大类化合物共36种;从病株检测到12大类化合物共37种.病株特有挥发物12种,即正十六烷、癸醚、butyl octyl phthalate、正十八烷、己二酸二异丁酯、正十五碳醛、十六烷酸甲酯、2-methylhexacosane、二苯甲酮、雪松醇、正二十烷和叶绿醇;病/健株中共有但含量差异显著的挥发物6种,即1-tricosene、octadecanal、萘、植酮、正十二烷醇和2,6-二叔丁基对甲酚.嗅觉选择实验表明,在50 μL/L浓度下,仅叶绿醇对白背飞虱成虫(健康与饲毒)表现出显著的驱避活性.[结论]SRBSDV的侵染改变了水稻挥发物的组分,且不同品种、不同生育期挥发物组分均具差异.叶绿醇为孕穗期荣优华占病株特有挥发物,对健康与饲毒白背飞虱均有驱避活性,是否有助于促进该病毒传播,尚需进一步验证.
