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包膜糖蛋白

包膜糖蛋白的相关文献在1989年到2022年内共计112篇,主要集中在基础医学、内科学、分子生物学 等领域,其中期刊论文84篇、会议论文5篇、专利文献117872篇;相关期刊52种,包括生物技术通讯、国际免疫学杂志、微生物与感染等; 相关会议4种,包括纪念中国微生物学会成立六十周年大会暨2012年中国微生物学会学术年会、2010新型疫苗研发及疫苗质量控制与安全性评价研讨会、黑龙江省免疫学会成立十周年学术会议等;包膜糖蛋白的相关文献由334位作者贡献,包括王志玉、凌虹、陈薇等。

包膜糖蛋白—发文量

期刊论文>

论文:84 占比:0.07%

会议论文>

论文:5 占比:0.00%

专利文献>

论文:117872 占比:99.92%

总计:117961篇

包膜糖蛋白—发文趋势图

包膜糖蛋白

-研究学者

  • 王志玉
  • 凌虹
  • 陈薇
  • 侯利华
  • 吕欣
  • 吴诗坡
  • 姚敏
  • 尹文
  • 庄敏
  • 徐俊杰
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 陈瑶; 韩亚坤; 吴强; 何仁亮
    • 摘要: 感觉神经病是艾滋病患者常见的一种并发症,目前临床医生对人类免疫缺陷病毒(HIV)感觉神经病的临床表现和发生机制认识不足,以及缺乏相应的治疗手段,使得大多数患者无法得到有效的治疗.本文就HIV相关感觉神经病的危险因素、机制和治疗等相关进展进行综述,旨在提高医务人员对HIV相关感觉神经病的诊治水平.
    • 陈露燕; 李伟; 徐佳露; 陶然; 李华美; 刘丽芳; 尚世强
    • 摘要: Objective To study the relationship between human cytomegalovirus (HCMV) envelope glycoprotein gene H and clinical features of children with congenital cytomegalovirus infection. Methods A cohort study was conducted. Newborns diagnosed with congenital cytomegalovirus infection, hospitalized in the Department of Neonatology and Neonatal Intensive Care Unit (NICU) of the Children′s Hospital, Zhejiang University School of Medicine, were included from July 2013 to December 2015. HCMV‐DNA gH typing in urine, sputum or blood was conducted. Patients then were divided into gH1 group and gH2 group according to gH genotypes. Patients′data during hospitalization in newborn and 3-5 years of follow‐up were collected. The relationships between gH genotype and clinical manifestations, laboratory examinations, hearing loss and neurological prognosis were analyzed by chi‐square test, t test and non‐parametric test. Results A total of 21 cases were enrolled as congenital HCMV infection and followed‐up for 3-5 years. Among them, 14 (67%) were gH1 type and 7 (33%) were gH2 type. No mixed infection was found. In the two groups, there were no significant differences in the ratio of males (9/14 vs. 3/7, P=0.397), or birth weight ((2 609±686) vs. (3 021±451) g, t=-1.436, P=0.167). Gestational age of gH1 group was younger than that of gH2 group (38 (29-40) vs. 39(38-40) weeks, Z=-2.18, P=0.029). Moderate to severe hearing loss detected by neonatal auditory brainstem response were found in 40 ears (20 cases). It was higher in gH1 group than that in gH2 group (4/22 vs. 0/18, χ2=5.145, P=0.023). In the imaging examination of the nervous system, the Alarcon score of gH1 group was lower than that of gH2 group (0.4±0.3 vs. 1.3±1.1, t=-2.459,P=0.024). No significant statistical difference was found in the probability of motor or language development lag in gH2 group and gH1 group (4/7 vs. 4/14, P=0.346). Conclusions Compared with gH2 infection, gH1 infection in children has a younger gestational age. The major type of hearing loss in neonatal period is gH1 infection. Children with gH2 congenital infections are more likely to suffer from nervous systems damage.%目的 探讨人巨细胞病毒(HCMV)gH基因分型与先天性巨细胞病毒感染临床特征的关系.方法 采用队列研究,选取2013年7月至2015年12月在浙江大学医学院附属儿童医院新生儿科及新生儿重症监护室(NICU)住院确诊为先天性巨细胞病毒感染的21例患儿,检测尿液、痰液或血液HCMV gH基因分型,根据gH基因分型结果将患儿分为gH1组和gH2组.收集患儿新生儿期住院资料和3~5年随访资料.应用χ2检验、t检验、非参数检验分析gH基因分型与患儿临床表现、实验室检查、听力损失、神经系统预后的相关性.结果 21例患儿中gH1型14例(67%)、gH2型7例(33%),未发现混合感染病例.两组男性比例(9/14比3/7,Fisher概率法,P=0.397)、出生体重[(2 609±686)比(3 021±451)g,t=-1.436,P=0.167]差异均无统计学意义.gH1组出生胎龄小于gH2组[38(29~40)比39(38~40)周,Z=-2.18,P=0.029].40耳(20例)进行新生儿期脑干诱发电位听力测试,gH1组中重度及以上听力损失比例高于gH2组(4/22比0/18,χ2=5.145,P=0.023).神经系统影像学检查Alarcon评分gH1组低于gH2组[(0.4±0.3)比(1.3±1.1)分,t=-2.459,P=0.024].gH2组出现运动或语言发育落后的概率与gH1组差异无统计学意义(4/7比4/14,Fisher概率法,P=0.346).结论 gH1型感染较gH2型感染新生儿出生胎龄小.新生儿期中重度及以上听力损失以gH1型感染为主.gH2型先天性感染患儿出现神经系统损害的可能性较大.
    • 闻雁波; 吴诗坡; 侯利华; 陈薇
    • 摘要: 目的:制备重组可溶性截短型马尔堡病毒包膜糖蛋白(MAGP),并验证其抗原性.方法:PCR扩增MAGP截短片段MAGP40-289、MAGP148-526、MAGP240-526和MAGP258-526,克隆至原核表达载体pET-32a并转化至大肠杆菌进行诱导表达,经亲和层析、阴离子交换层析获得纯度良好的可溶性截短蛋白抗原,利用ELISA试验对该蛋白的抗原性进行验证.结果:截短抗原MAGP240-526在大肠杆菌中可溶性表达良好,表达量约17 mg/L;Western印迹显示该抗原能与Ad5-MAGPopt免疫后的小鼠血清发生特异反应.结论:经原核系统表达的截短型MAGP具有良好的抗原性,可用于检测相关疫苗激发的抗MAGP抗体水平或评价抗MAGP的结合活性.
    • 张振勇; 夏宁邵; 李少伟; 李婷婷; 乔佳明; 张玉云; 高双全; 姚巧缤; 李泽凯; 张芝晴; 顾颖
    • 摘要: 目的 利用杆状病毒-昆虫细胞表达系统建立高效分泌表达人类免疫缺陷病毒(HIV)-1 Env gp120糖蛋白的方法,并对其理化性质及免疫原特性进行分析.方法 选择B亚型HIV-1 NL4-3 gp120进行蛋白克隆设计,将目的蛋白基因序列克隆到杆状病毒转移载体pAcgp67B中pH启动子的下游,构建成重组转移载体pAc-gp120,利用同源重组的方式在宿主细胞内获得重组杆状病毒,并在High FiveTM昆虫细胞中表达gp120蛋白.之后使用酶联免疫吸附试验、分析超离和分子排阻色谱进行理化性质分析,并通过小鼠免疫实验分析其免疫原性.结果 建立昆虫细胞表达系统制备和纯化重组HIV-1 gp120蛋白的方法,最终获得纯度约90%的gp120蛋白,多批次纯化后鉴定产量约为13 mg/L;酶联免疫吸附测定、分析超离和分子排阻色谱性质分析试验显示,重组HIV gp120蛋白在溶液有良好的抗原性并且在溶液中较为均一;通过弗氏佐剂与目的蛋白混合免疫BALB/c小鼠,免疫血清对NL4-3病毒具有一定程度中和活性,表明gp120蛋白能有效刺激机体产生免疫应答.结论成功建立了简便、可放大的HIV gp120蛋白表达和纯化方法,获得较为均一的、免疫原性良好的gp120抗原,为进一步开展HIV疫苗研究工作奠定了基础.%Objective To establish an efficient baculovirus-insect cell expression system for the production of human immunodeficiency virus-1 ( HIV-1 ) envelope glycoprotein gp120 and to evaluate the physiochemical properties, antigenicity and immunogenicity of the recombinant protein. Methods The gene encoding HIV-1 NL4-3 gp120 was cloned into the downstream of pH promoter of the baculovirus transfer vec-tor pAcgp67B to construct the recombinant transfer vector pAc-gp120. Expression of the protein of interest was induced in baculovirus-infected High FiveTM insect cells. ELISA, analytical ultracentrifugation and size-exclusion chromatography were carried out to characterize physicochemical properties of the expressed gp120 protein. Immunogenicity of the recombinant gp120 protein was analyzed by HIV neutralization assay after im-munizing BALB/c mice with it. Results The recombinant HIV-1 gp120 protein was successfully obtained from the established insect cell expression system with a purity of more than 90% and a mean yield of 13 mg/L in four batches. That recombinant HIV-1 gp120 protein was characterized by homogeneity in solution and possessed a good immunoreactivity to neutralizing antibodies and antisera against HIV. Immunogenicity analysis in BALB/c mice demonstrated that the recombinant gp120 protein could induce effective immune re-sponses against HIV-1 NL4-3. Conclusion A simple and scalable approach to obtain homogeneous and im-munogenic HIV-1 gp120 antigen is successfully established, which will promote further investigation of HIV vaccine candidates.
    • 陈冰霞; 徐俊; 马伟峰
    • 摘要: 艾滋病是由人类免疫缺陷病毒(HIV)引起的一种烈性传染病,危害极大.病毒的包膜蛋白gp120及其受体CD4、辅助受体CXCR4在病毒感染中起着重要的作用.其中,gp120的Phe43口袋结构和Arg59是其与CD4分子相互作用的重要部位.HIV小分子进入抑制剂因能在HIV感染细胞前即发挥抑制病毒的作用,成为近年来抗HIV药物研究的热点.Phe43口袋结构是小分子进入抑制剂的重要靶点,目前研究热点化合物主要有BMS-378806、BMS-488043及其类似物、NBD-556及其类似物.靶向CXCR4也是研究HIV小分子进入抑制剂的一个重要方向.%AIDS is an infectious disease caused by human immunodeficiency virus(HIV)and has done great harm to human beings. The envelope glycoprotein surface subunit gp120 and its receptor CD4 and coreceptor CXCR4 play important roles in HIV-1 en?try. The Phe43 pocket and Arg59 of gp120 are two important regions that interact with CD4. Recently,HIV small-molecule entry inhib?itors have become a hot topic in anti-HIV drug research,which are able to inhibit the virus before the cells are infected. The Phe43 pocket is an attractive target and the study of Phe43 pocket-targeting small-molecule entry inhibitors is underway,including BMS-378806,BHS-488043 and its analogs,and NBD-556 and its analogs. Besides,CXCR4 antagonist is another important approach.
    • 冯伟; 张尧; 张晓晓; 应旗康; 刘梓谕; 吴兴安; 王芳
    • 摘要: 目的:构建汉滩病毒包膜糖蛋白基因的真核表达载体,并加入可增强免疫应答效应的细胞因子CD40L基因,检测其可否在真核细胞中表达.方法与结果:参照GenBank中汉滩病毒M基因和小鼠CD40L的全基因序列设计引物,通过聚合酶链反应(PCR)获得M和CD40L基因片段,将其与pCI-neo载体相连,测序证实该载体构建成功后,将此真核表达载体以脂质体转染法转染至哺乳动物细胞CHO-K1中,利用间接免疫荧光法(IFA)检测发现M基因和CD40L基因可以同时表达于CHO-K1细胞中.结论:构建了带有CD40L基因的汉滩病毒包膜糖蛋白重组质粒并获得表达,为深入研究汉滩病毒感染后包膜糖蛋白引起的特异性免疫应答规律奠定了实验基础.%Objective:The eukaryotic expression vector of Hantaan virus glycoprotein was constructed and added with mouse CD40L gene that could strengthen mouse immune response,in order to detect whether or not it could express in the eukaryotic cell.Methods & Results:Primers were designed referring to M gene sequence of Hantaan virus and mouse CD40L gene in the GenBank.Then M gene and mouse CD40L gene were obtained through PCR,and cloned into eukaryotic expressing vector pCI-neo vector.After sequenced,the eukaryotic expressing vector was transfected into eukaryotic cells CHO-K1 with liposome method.It was found that specific fluorescence appeared in the CHO-K1 after transfection by indirect immunofluorescence assay(IFA).Conclusion:The results showed that the eukaryotic expressing vector of Hantaan virus glycoprotein conjugated with mouse CD40L was successfully constructed and expressed,to deeply investigate the immune response of Hantaan virus glycoprotein.
    • 摘要: 背景:埃博拉病毒(EBOVs)能够引发严重的出血热,具有极高的致死率。目前世界上尚无批准上市的疫苗以及对该病毒感染的有效治疗手段。有研究表明,体液免疫和细胞免疫反应是控制埃博拉病毒感染的关键,而且CD8+T细胞在介导疫苗引发的免疫保护方面发挥着重要作用。
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