摘要:
目的 探讨姜黄素对非酒精性脂肪肝病( NAFLD)大鼠肠道 sIgA及氧化应激的影响.方法将雄性SD大鼠30只随机分为3组:正常对照组10只、高脂组10只、高脂+姜黄素组10只.高脂组、高脂+姜黄素组给予高脂饲料喂养16周,其中高脂+姜黄素组在高脂喂养8 周后,给予姜黄素灌胃200 mg/( kg· d),1次/d.光学显微镜观察小肠黏膜及肝脏病理变化;鲎试剂测定门静脉脂多糖( LPS)水平,酶联免疫法测定肠道sIgA含量,分光光度法测定血浆二胺氧化酶( DAO)活性,羟胺法测定小肠超氧化物歧化酶( SOD)活性,比色法测小肠丙二醛(MDA)水平,全自动生化仪检测血清ALT、AST.结果 光镜下,对照组大鼠小肠黏膜及肝组织无明显异常,高脂组小肠黏膜绒毛稀疏,部分绒毛出现断裂、缺损,肝细胞内可见较多脂滴浸润,并有气球样变,高脂+姜黄素组小肠绒毛比较完整,绒毛缺失减少,肝细胞脂肪变明显减轻.与对照组比较,高脂组大鼠血AST、ALT、DAO、LPS及小肠MDA水平明显升高,小肠SOD活性及肠道sIgA下降,差异均有统计学意义(P<0.05);与高脂组比较,高脂+姜黄素组大鼠血AST、ALT、DAO、LPS及小肠MDA降低,SOD活性及肠道sIgA升高,差异均有统计学意义(P<0.05).结论 姜黄素可能通过增加肠道sIgA水平、改善肠黏膜氧化应激状态而减轻NAFLD时肠黏膜屏障损伤.%Objective To investigate the effects of curcumin (Cur) on intestinal sIgA and oxidative stress in rats with nonalcoholic fatty liver disease (NAFLD).Methods Thirty male SD rats were randomly divided into normal control group (Group NC), high fat group (Group HF) and Group HF+Cur.High fat diet were given in Group HF group and HF+Cur for 16 weeks.After 8 weeks of high fat feeding in Group HF +Cur, curcumin [200 mg/(kg· d)] were given with lavage for 8 weeks.The pathological changes of intestinal mucosa and liver were observed by optical microscope .The lipopolysaccharide (LPS) of portal vein blood was measured by limulus test .Intestinal sIgA levels were determined by en-zyme linked immunosorbent assay (ELISA).The activity of plasma diamine oxidase (DAO) was determined by spectro-photometric method.The activity of SOD in intestine was measured by hydroxylamine method , and the content of MDA in intestine was measured by colorimetry .The serum levels of alanine aminotransferase ( ALT) and aspartate aminotrans-ferase (AST) were detected by automatic biochemical analyzer .Results Under light microscope , there was no obvious abnormality in small intestinal mucosa and liver in Group NC .In Group HF, intestine mucosa villi were sparse , some of them were broken and defect ; fat droplets infiltration and balloon -like changes in hepatocyte could be seen .In Group HF+Cur, intestine villi were more complete than Group HF , the loss of villi was decreased , and hepatocellular steatosis was significantly reduced.Compared with Group NC, the blood levels of AST , ALT, DAO, and LPS and MDA contents of in-testine in HF group were obviously increased , SOD activity of intestinal and sIgA levels of intestinal were decreased (P<0.05).Compared with HF group, the blood levels of AST, ALT, DAO and LPS, and MDA content of intestine in Group HF+Cur were significantly lowered; and SOD activity and sIgA level in intestine were significantly elevated (P<0.05). Conclusion Curcumin can reduce intestinal mucosal barrier injury by increasing sIgA of intestinal mucosa and improving oxidative stress of intestinal mucosa in rats with NAFLD .