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HLA-G

HLA-G的相关文献在1998年到2022年内共计152篇,主要集中在基础医学、肿瘤学、妇产科学 等领域,其中期刊论文152篇、相关期刊103种,包括科学技术与工程、国际免疫学杂志、现代生物医学进展等; HLA-G的相关文献由429位作者贡献,包括颜卫华、林爱芬、许惠惠等。

HLA-G—发文量

期刊论文>

论文:152 占比:100.00%

总计:152篇

HLA-G—发文趋势图

HLA-G

-研究学者

  • 颜卫华
  • 林爱芬
  • 许惠惠
  • 甄宏
  • 范丽安
  • 谢庆玲
  • 姚元庆
  • 朱晓明
  • 洪梅
  • 温见燕
  • 期刊论文

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    • Xianpeng Jiang; Catherine C. Baucom; Toby Jiang; Robert L. Elliott
    • 摘要: Purpose: HLA-G binds to the inhibitory receptors of uterine NK cells and plays an important role in protection of fetal cells from maternal NK lysis. HLA-G also mediates tumor escape, but the immunosuppressive role is often neglected. These studies have focused on the examination of HLA-G expression in human breast and ovarian carcinoma and HLA-G immunosuppressive role in NK cytolysis. Methods: We examined HLA-G expression in breast and ovarian carcinoma cell lines by real time PCR, ELISA and immunofluorescent staining, and in frozen breast and ovarian carcinoma tissues by immunohistochemistry (IHC). We treated the breast cancer cell lines with anti-human HLA-G antibody or progesterone. Then, NK cytolysis was measured by using MTT assay. Results: We find breast and ovarian cancer cell lines increase the expression of HLA-G mRNA and protein, compared to normal cells. IHC shows that 100% of frozen breast and ovarian carcinoma tissues overexpress HLA-G protein. HLA-G IHC scores of breast and ovarian carcinoma are significantly higher than normal breast and ovarian tissues, respectively (both p < 0.01). Blocking HLA-G of the breast cancer cells by the antibody increases NK cytolysis. Progesterone upregulates HLA-G mRNA and protein of human breast cancer cell lines. The increased HLA-G expression by progesterone suppresses the NK cytolysis. Conclusion: Human breast and ovarian carcinoma overexpress HLA-G immunosuppressive molecules. Blocking HLA-G protein by antibody improves the cytolysis of NK cells against human breast cancer cell lines. In contrast, upregulation of HLA-G expression by progesterone impairs NK cytolytic function. Thus, HLA-G is a new immune checkpoint protein and potential cancer immunotherapeutic target.
    • 刘倩; 哈提拉·吐尔逊; 阿仙姑·哈斯木
    • 摘要: 目的探索宫颈癌细胞中HLA-G表达差异对NK细胞表面活化性受体的影响。方法实时定量PCR(real-time PCR)及免疫印迹法(Western-Blot WB)检测宫颈癌细胞及正常宫颈细胞中HLA-G的表达差异。利用慢病毒载体构建HLA-G稳定下调的宫颈癌细胞株,并与NK细胞共培养,CCK8法检测共培养后宫颈癌细胞增殖能力的变化,流式细胞术检测宫颈癌细胞凋亡水平。同时,流式细胞术检测共培养后NK细胞表面活化性受体NKG2D、NKP30的表达水平及颗粒素(Granzyme)、穿孔酶(Perforin)的表达差异。结果RT-PCR及WB法检测结果显示:与H8细胞相比,C33a、SiHa细胞中HLA-G的表达显著升高(P0.05)。结论HLA-G在宫颈癌细胞中高表达,HLA-G表达升高可促进活化性受体的表达,增强NK细胞对宫颈癌细胞的杀伤作用。
    • 杨帆; 尚炳亮; 刘静; 姚田军; 周幸春; 王禾; 张波
    • 摘要: 目的 探索线粒体转录因子A(TFAM)表达下调促进膀胱癌细胞逃避NK细胞杀伤的作用及机制.方法 免疫组化检测膀胱癌及癌旁组织中TFAM的表达,统计学分析其表达差异及膀胱癌组织中TFAM表达水平与膀胱癌患者预后的相关性.利用慢病毒载体构建TFAM稳定干涉及过表达的膀胱癌细胞株并与NK细胞共培养,ELISA及LDH释放实验检测TFAM表达对NK细胞活化及杀伤的影响.qPCR、Western-blot及流式细胞术检测膀胱癌细胞中TFAM表达对人类的细胞抗原-G(HLA-G)表达的影响.同时,利用HLA-G中和抗体阻断TFAM干涉细胞与NK共培养体系中HLA-G功能,或者利用重组rHLA-G增强TFAM过表达膀胱癌细胞与NK细胞共培养体系中HLA-G功能后,检测膀胱癌细胞TFAM表达是否是通过HLA-G影响NK细胞的活化及杀伤.qPCR、Picogreen与Mitotracker共染膀胱癌细胞检测TFAM表达对线粒体DNA(mtDNA)应激发生的影响.利用DNA酶Ⅰ去除TFAM稳定干涉膀胱癌胞质mtDNA或者鱼藤酮阻断TFAM过表达膀胱癌细胞线粒体功能后,检测TFAM是否是通过mtDNA应激影响HLA-G的表达.结果 与癌旁组织相比,TFAM在膀胱癌组织中的表达显著减少,且TFAM表达越低的膀胱癌患者其预后越差.在膀胱细胞中干涉TFAM表达可通过上调HLA-G表达来抑制NK细胞的活化及杀伤.反之,在膀胱癌细胞中过表达TFAM可通过抑制HLA-G的表达来促进NK细胞的活化及杀伤.在TFAM干涉的膀胱癌细胞中加入DNA酶Ⅰ可显著抑制TFAM干涉诱导的HLA-G表达,在TFAM过表达的膀胱癌细胞中加入鱼藤酮可恢复TFAM过表达导致HLA-G表达下调.结论 TFAM表达下调诱导mtDNA应激促进HLA-G表达来抑制NK细胞的活化及杀伤.
    • 刘翠莲; 崔澂
    • 摘要: 子痫前期(PE)是一种常见的妊娠特有并发症,其病因及发病机制与免疫因素有关,而人类白细胞抗原G(HLA-G)在母胎免疫调节中发挥重要作用.HLA-G具有启动子独特性及限制多态性两种基因特性,胎儿滋养细胞HLA-G分子低表达是介导PE发生的关键环节,可能由细胞胞啃机制受阻、获取HLA-G能力下降所致,继而通过Th1/Th2平衡向Th1偏移、滋养细胞浸润能力受损,造成胎盘浅着床和子宫螺旋动脉重铸不足,促进PE的发生发展.
    • 赵桂增; 张晨光
    • 摘要: 人类白细胞抗原G(HLA-G)是一种非经典的人类主要组织相容性复合物(MHC) Ib类分子,是机体内重要的免疫耐受分子,有助于免疫逃逸或免疫细胞无能.HLA-G可限制性表达,生理条件下,HLA-G分布于免疫豁免组织细胞上,但在肿瘤及病毒感染等病理状态下,HLA-G在相应组织细胞上可获诱导性表达.因此,HLA-G的功能可能是有益的,因为当妊娠或移植时,HLA-G可保护其免受机体免疫系统伤害;同时,HLA-G也是有害的,因为它的免疫耐受功能同样可以被肿瘤或者病毒利用,从而保护它们免受机体的攻击,造成对机体的危害.近年来,人们对HLA-G参与免疫调节进行了大量研究,本文就HLA-G在移植和肿瘤免疫耐受方面作一综述.
    • 陈霞林; 尹治军; 王华杰
    • 摘要: 目的:观察独活寄生汤治疗肝肾亏虚型类风湿关节炎的临床疗效.方法:选取2016年1月—2019年12月邵阳市第一人民医院中医科收治的60例肝肾亏虚型类风湿关节炎患者,随机分为治疗组和对照组,各30例.对照组口服来氟米特治疗,治疗组给予独活寄生汤加减治疗.结果:治疗组与对照组经过治疗DAS28评分下降(P<0.05),但两组相比无差异无统计学意义(P>0.05);治疗组与对照组经过治疗,血清sHLA-G均升高(P<0.05),但两组相比差异无统计学意义(P>0.05).结论:单用独活寄生汤治疗肝肾亏虚型类风湿关节炎有效;经过治疗HLA-G表达有上升,控制或延缓HLA-G下降可能阻止类风湿关节炎的发生发展,HLA-G有可能成为治疗类风湿关节炎新的作用靶点.
    • 蒋莉; 吴长亮; 邓静; 王朋朋; 吴健林; 吴继周
    • 摘要: 目的:探讨非经典组织相容性复合体(MHC)Ⅰ类分子人类白细胞抗原(HLA)-G基因多态性与广西肝癌家族聚集性的相关性.方法:采用病例对照研究设计,收集广西地区140例来自肝癌家族的家庭成员作为实验组,同期选择相同性别、年龄±5岁、相同民族、相同生活环境、相同生活习惯的140例无癌家族家庭成员作为对照组,通过聚合酶链反应(PCR)结合测序法检测HLA-G基因多态性.结果:实验组-14 bp/-14 bp基因型频率分布高于对照组,+14 bp/+14 bp、+14 bp/-14 bp低于对照组(P0.05).结论:HLA-G基因14 bp插入/缺失多态性可能与肝癌家族聚集性有关.
    • 刘侃; 王秋明; 宋婉玉; 武海英
    • 摘要: Objective To investigate the expression and role of regulatory plasma cells in gravidas with systemic lupus erythematosus ( SLE) . Methods Gravidas with SLE were enrolled in Henan Provincial People's Hospital from April 2013 to April 2018. They were divided into three groups including pregnancy control group, SLE stable group and SLE deterioration group. The ratio of CD3-LAG-3+CD138high regulatory plasma cells was detected by flow cytometry. The concentrations of soluble human leukocyte antigen-G ( sHLA-G) and anti-nuclear antibody Ig were detected by ELISA. Lymphocytes in peripheral blood of SLE deterioration group were isolated, and then cultured in RPMI1640 medium containing 10% fetal bovine ser-um and stimulated with HLA-G. Results Flow cytometry showed that the proportion of regulatory plasma cells in SLE stable group was (2. 483±0. 1318)% and that in SLE deteriorating group was (1. 662± 0. 1304)%. There was a significant difference between the two groups (t=4. 431, P=0. 0013). The con-centrations of sHLA-G in SLE stable group and SLE deteriorating group were (36. 50±3. 510) ng/ml and (16. 50±2. 405) ng/ml, and the difference between the two groups was statistically significant (t=4. 701, P=0. 0008). Correlation analysis showed that the concentration of sHLA-G was positively correlated with the proportion of regulatory plasma cells (r=0. 7471, P=0. 0009). The results of in vitro experiment showed that the proportions of B cells and regulatory plasma cells were ( 7. 573 ± 0. 6539 )% and ( 1. 593 ± 0.1879)% in SLE deterioration group and (3. 732±0. 7178)% and (2. 503±0. 2921)% in HLA-G group with statistical differences between the two groups (t=3. 957, P=0. 0027;t=2. 620, P=0. 0256). Conclusions The proportion of regulatory plasma cells and the concentration of sHLA-G were significantly decreased in pregnant patients with SLE, which was closely related to disease severity. HLA-G played an important role in promoting the proliferation of regulatory plasma cells.%目的 探讨调节性浆细胞在系统性红斑狼疮(systemic lupus erythematosus,SLE)合并妊娠患者中的表达及作用.方法 选取2013年4月至2018年4月来我院就诊的SLE合并妊娠的患者,分为对照妊娠组、SLE稳定组和SLE恶化组.流式细胞术检测CD3-LAG-3+CD138 high调节性浆细胞的比例;ELISA检测可溶性人白细胞抗原-G(sHLA-G)和抗核抗体Ig的浓度;分离SLE恶化组外周血中淋巴细胞,置于含有10%胎牛血清的RPMI 1640培养基中培养,并给予HLA-G蛋白刺激.结果流式细胞术结果显示SLE稳定组调节性浆细胞的比例为(2.483±0.1318)%,SLE恶化组的比例为(1.662±0.1304)%,两组间差异有统计学意义(t=4.431,P=0.0013);SLE稳定组的sHLA-G浓度为(36.50±3.510)ng/ml,SLE恶化组浓度为(16.50±2.405)ng/ml,两组间差异有统计学意义(t=4.701,P=0.0008);相关性分析的结果显示sHLA-G的浓度与调节性浆细胞的比例呈正相关(r=0.7471,P=0.0009);体外试验结果显示SLE恶化组B细胞和调节性浆细胞的比例分别为(7.573±0.6539)% 和(1.593±0.1879)%,HLA-G组的比例分别为(3.732±0.7178)% 和(2.503±0.2921)%,差异均有统计学意义(t=3.957,P=0.0027;t=2.620,P=0.0256).结论 SLE合并妊娠患者体内调节性浆细胞比例和sHLA-G浓度均显著降低,且与病情严重程度紧密相关,HLA-G具有促进调节性浆细胞比例升高的重要作用.
    • 陈昕涛; 高倩闵; 姚厚山
    • 摘要: 人类白细胞抗原G(Human Leucocyte Antigen-G,HLA-G)是一种非典型性的MHC-I类分子.根据研究数据显示,HLA-G具有免疫细胞的功能,与肿瘤、生殖以及器官移植等免疫有关,可以抑制自然杀伤细胞和细胞毒性T淋巴细胞,参与母胎免疫耐受的形成.由于HLA-G能通过抑制自然杀伤细胞,达到诱导肿瘤抗原特异性T细胞消亡等的特性,目前已成为肿瘤癌症治疗领域的研究重点.在临床上建立一种非侵袭性、简单而有效的血清学方法用于对结直肠癌的诊断、良性和恶性肠道疾病的有效区分以及结直肠癌的治疗有重要的意义.本文针对HLA-G在结直肠癌的诊断以及预后中的作用进行研究分析,并对其临床效果进行评估.
    • Bernardin Ahouty; Mathurin Koffi; David Courtin; Ilboudo Hamidou; Didier Sokouri; Innocent Abé; Laure Gineau; Thomas Konan; Lingué Kouakou; Tidou Abiba Sanogo; Enock Matovu; Bruno Bucheton; Vincent Jamonneau; Simon-Pierre N’Guetta
    • 摘要: Human African trypanosomiasis (HAT), or sleeping sickness, caused by Trypanosoma brucei gambiense, is associated with diverse clinical outcomes. Host’s genetic factors involved in immunity are potential factors that can regulate infection. Genetic polymorphisms within HLA-G could influence the level of HLA-G expression and therefore play a critical role in infection outcomes. The goal of our study was to investigate the association of 14 bp Indel HLA-G polymorphism with the susceptibility/resistance to HAT. DNA samples were collected from 119 cases, 221 controls and 43 seropositive individuals living in Ivorian HAT foci. The 14 bp Indel polymorphism was determined by PCR. Homozygous individuals for 14 bp insertion had a lower risk of progressing to active HAT (p = 0.012, OR = 0.27, 95% CI: 0.09 - 0.8). Moreover, the frequency of 14 bp insertion homozygous genotype was higher in the seropositive group (11%) than in the HAT cases group (3%) (p = 0.043, OR = 0.27, 95% CI: 0.07 - 0.99), which suggested a protective effect of 14 bp insertion homozygous genotype. Genetic polymorphisms in HLA-G may be associated with a variable risk to develop HAT. The 14 bp insertion appears to favour the occurrence of long-lasting T. b. gambiense latent infections.
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