摘要：
In order to provide reference for the related product development of Se-enriched Lentinus edodes,the selenium tolerant ability of low temperature type Lentinus edodes in Qinling-Bashan mountainous area was investigated and the Se-enriched liquid medium of L.edodes was optimized.Adopting L.edodes strains (9608#) as experimental materials,the most suitable concentration of Na2 SeO3 for mycelial growth was determined,and the Se-enriched liquid medium of L.edodes strains was optimized by the experiments of initial experimental design of Plackett-Burman,path of steepest ascent,Box-Behnken design and response surface analysis.The results showed that the most suitable concentration of Na2SeO3was 20.00 mg/L,and the optimum Se-enriched liquid medium were as follows:potatoes 288.78 g/L,wheat bran 64.56 g/L,maltose 15.00 g/L,yeast extract 10.00 g/L,KH2PO4 0.80 g/L,MgSO4·7H2O 2.00 g/L,Na2SeO3 20.00 mg/LAnd validation results showed that the mycelial biomass of Se-enriched L.edodes (9608#) was 66.34 g/L,compared with the theoretical value,the relative error of 2.05 ％.Therefore,this method was feasible and the conclusion of this study was reliable.%考察秦巴山区低温型香菇的硒耐受力,优化其在富硒条件下的培养基配方,拟为富硒香菇的相关产品开发提供参考依据.以秦巴山区规模化栽培的低温型香菇9608#菌株为试材,根据菌丝平均生长速度筛选其耐受Na2 SeO3的最佳浓度;以菌丝体生物量为主要指标,通过Plackett-Burman实验、最陡爬坡实验、Box-Behnken响应面实验及验证实验,研究香菇9608#菌株最优的富硒液体培养基.结果表明,香菇9608#菌株耐受Na2Se03的最佳浓度为20.00 mg/L,其最佳富硒液体培养基为:马铃薯288.78g/L、麦麸64.56 g/L、麦芽糖15.00 g/L、酵母膏10.00g/L、KH2PO4 0.80 g/L、MgSO4·7H20 2.00 g/L、Na2SeO3 20.00 mg/L.以此培养基对实验结果进行验证,菌丝生物量可达66.34 g/L,实测值与拟合值相比,相对误差约为2.05％,说明本研究方法可行、结论可靠.
