fiber

摘要： In 2021,the global economy gradually recovered,and China’s economy also achieved sustained growth,the growth rate ranking among the highest in the world.The internal and external demand for China’s textile and garment has picked up,and the advantages of the complete industrial system have been fully brought into play.

摘要： Strigolactones(SLs)are a new type of plant endogenous hormones that have been found to regulate plant growth and architecture.At present,some genes related to the biosynthesis and signaling pathway of SLs have been isolated in plants such as Arabidopsis thaliana,Pisum sativum and Oryza sativa.However,the signaling pathway and specific mechanism of SLs in cotton remain unclear.In this study,we identified the SLs signaling gene GhMAX2 and demonstrated its function in plant growth and architecture in Gossypium hirsutum.Bioinformatics analysis showed that GhMAX2 mainly consists of anα-helix and a random coil and includes a large number of leucine-rich repeats.GhMAX2 was highly expressed in root,stem,flower,and fibers at 20 days post-anthesis(DPA).GhMAX2 promoter-drivenβ-glucuronidase expression was present exclusively in the root,main inflorescence,flower,and silique.Subcellular localization showed that GhMAX2 is targeted to the nucleus.Heterologously expressed GhMAX2 can rescue the phenotype of Arabidopsis max2-1 mutant,indicating that the function of MAX2 is highly conserved between G.hirsutum and A.thaliana species.In addition,the knockdown expression of GhMAX2 in cotton resulted in significantly reduced plant height,slow growth,short internodes,and reduced fiber length.These findings indicate that GhMAX2 probably contributes to plant growth,architecture and fiber elongation in cotton.The study reveals insights into the roles of GhMAX2-mediated SL/KAR signaling in cotton and provides a valuable foundation for the cultivation of cotton plants in the future.

摘要： The article improves the quality of raw cotton by creating a new transfer device structure for the transportation of cotton in long-distance riots, located in the main building of the ginnery. Both foreign and domestic separator cleaners have been studied. Experiments were carried out on prototype transfer device and the results were obtained. The cleaning efficiency was determined by sampling the cotton entering the separator in the moving device and exiting after the inclined vibrating mesh surface installed after the separator.

摘要： Due to their interesting properties, bamboo fibers are more and more used as reinforcements in polymer matrices as a substitute for synthetic fibers. For their future service life, it is important to understand their physical and mechanical behavior over time in order to control the aging phenomenon within this fiber. The paper analyzed the influence of the age of the bamboo thatch and the vertical position of the Bambusa vulgaris species cultivated in Cameroon on the physicomechanical properties of the fibers extracted from the thatch. Fibers were mechanically extracted from three bamboo culms aged respectively 3 years (BV3), 4 years (BV4) and 5 years (BV5). The culms were thus identified according to their number of ramifications, and were felled no abated for a total of three culms. A section of about one meter on each of the parts (lower part, middle part, upper part) of these three culms was made for the opposite technological studies. Each age was therefore represented by three portions of thatch, one from the upper part, one from the middle part and the last from the lower part of the thatch, all giving a total number of nine samples taken and marked BV3inf, BV3moy, BV3sup, BV4inf, BV4moy, BV4sup, BV5inf, BV5moy, BV5sup before handling in the laboratory. Physical (density, moisture absorption rate) and mechanical (tensile tests according to DIN EN ISO 13934-1, natural durability) characterizations were used to better understand the mechanisms of this influence. In view of all the results obtained, the fiber from the upper part of the 3-year-old thatch (BV3sup) is the one with the best characteristics and is recommended for a better elaboration of bamboo fiber composites: (Density: 0.83;Absorption rate 11.7%;Young’s modulus: 7.4 GPa;Maximal stress: 64.3 MPa;Elongation at rupture: 1.1;Loss of mass natural durability: 7.63%).

摘要： Background:Alternative feedstuffs may contribute to reducing feed costs of pig production.But these feedstuffs are typically rich in fiber and resistant starch(RS).Dietary fibers and RS are fermented in the gastrointestinal tract(GIT)and modulate the microbial community.Certain microbes in the GIT can promote host health,depending on the type of fermentation substrates available.In this study,six alternative feedstuffs(three starchy:Okinawan sweet potato,OSP;yam,and taro,and three fibrous:wheat millrun,WMR;barley brewers grain,BBG;and macadamia nut cake,MNC)were evaluated for their in vitro digestibility and fermentation characteristics and their effects on pig’s hindgut microbial profile.After 2 steps of enzymatic digestion assay,residues were fermented using fresh pig feces as microbial inoculum,and gas production was recorded periodically for 72 h and modeled for fermentation kinetics.After fermentation,the residual liquid phase was analyzed for short-chain fatty acid(SCFA),and the solid phase was used to determine the nutrient’s digestibility and microbial community.Results:In vitro ileal digestibility of dry matter and gross energy was higher in starchy than fibrous feedstuffs.Total gas and SCFA production were significantly higher(P<0.001)in starchy feedstuffs than fibrous feedstuffs.Both acetate and propionate production was significantly higher(P<0.001)in all starchy feedstuffs than BBG and MNC;WMR was in between.Overall alpha diversity was not significantly different within and between starchy and fibrous feedstuffs.Beta diversity(measured using bray Curtis dissimilarity distance)of starchy feedstuffs was significantly different(P<0.005)than fibrous feedstuffs.Conclusion:Starchy feedstuffs acted as a substrate to similar types of microbes,whereas fibrous feedstuffs resulted in a more diverse microbial population.Such alternative feedstuffs may exert comparable beneficial effects,thus may be included in swine diets to improve gut health.

摘要： Microplastics(MPs)are ubiquitous in marine sedimentary environments.Their occurrence in horseshoe crabs and interactions with the sedimentary environment has not been determined.In this study,MPs,mostly microfibers,were found in all extracted gastrointestinal tract(GIT)samples of the juvenile Tachypleus tridentatus from the northern Beibu Gulf,China.The MP concentrations(4–53 items ind-1)were higher than those in most marine benthic invertebrates(<15 items ind-1)reported in Chinese waters,despite their generally low level in habitat sediments(9–1818 items kg-1).The correlation between the juveniles and sediments was not evident,but the abundance in juvenile horseshoe crab GITs decreased with ages.The findings were relevant to the life-history characteristics of the species,typically with limited dispersal capability and their spending nine years or longer time living in mangrove wetlands during low tides,with apparent ontogenetic changes in their diets.These baseline data enable a better understanding of MP availability in benthic macroinvertebrates,and the ecological risks present in the ecosystems.

摘要： Background:The majority of attenuated total reflection Fourier transform infrared(ATR FT-IR)investigations of cotton are focused on the fiber tissue for biological mechanisms and understanding of fiber development and maturity,but rarely on other cotton biomass comp on ents.This work examined in detail the ATR FT-IR spectral features of various cott on tissues/organs at reproductive and maturation stages,an a lyzed and discussed their biological implications.Results:The ATR FT-IR spectra of these tissues/organs were an a lyzed and compared with the focus on the lower wavenumber fingerprinting range.Six outstanding FT-IR bands at 1730,1620,1525,1235,1050 and 895 cm^(-1) represented the major C=O stretching,protein Amide I,Amide II,the O-H/N-H deformation,the total C-O-C stretching and the β-glycosidic linkage in celluloses,respectively,and impacted differently between these organs with the two growth stages.Furthermore,the band intensity at 1620,1525,1235,and 1050 cm^(-1) were exclusively and significantly correlated to the levels of protein(Amide I bond),protein(Amide II bond),cellulose,and hemicellulose,respectively,whereas the band at 1730 cm^(-1) was negatively correlated with ash content.Conclusions:The resulting observations indicated the capability of ATR FT-IR spectroscopy for monitoring changes,transportation,and accumulation of the major chemical components in these tissues over the cotton growth period.In other words,this spectral technology could be an effective tool for physiological,biochemical,and morphological research related to cotton biology and development.

摘要： High-performance flexible one-dimensional(1D)electrochemical energy storage devices are crucial for the applications of wearable electronics.Although much progress on various 1D energy storage devices has been made,challenges involving fabrication cost,scalability,and efficiency remain.Herein,a highperformance flexible all-fiber zinc-ion battery(ZIB)is fabricated using a low-cost,scalable,and efficient continuous wet-spinning method.Viscous composite inks containing cellulose nanofibers/carbon nanotubes(CNFs/CNTs)binary composite network and either manganese dioxide nanowires(MnO_(2) NWs)or commercial Zn powders are utilized to spinning fiber cathodes and anodes,respectively.MnO_(2) NWs and Zn powders are uniformly dispersed in the interpenetrated CNFs/CNTs fibrous network,leading to homogenous composite inks with an ideal shear-thinning property.The obtained fiber electrodes demonstrate favorable uniformity and flexibility.Benefiting from the well-designed electrodes,the assembled flexible fiber-shaped ZIB delivers a high specific capacity of 281.5 m Ah g^(-1) at 0.25 A g^(-1) and displays excellent cycling stability over 400 cycles.Moreover,the wet-spun fiber-shaped ZIBs achieve ultrahigh gravimetric and volumetric energy densities of 47.3 Wh kg^(-1) and 131.3 m Wh cm^(-3),respectively,based on both cathode and anode and maintain favorable stability even after 4000 bending cycles.This work offers a new concept design of 1D flexible ZIBs that can be potentially incorporated into commercial textiles for wearable and portable electronics.

摘要： Different optical materials used in textiles will show different effects of light response.Fiber optic fabric is a blend of optical fiber and textile fiber.By adding light-emitting diode at the end of fiber with high permeability,it can become self-luminous fiber fabric after electrification.During the day,the effect is the same as ordinary fabric.After being energized in the dark,it will emit dazzling light.

摘要： We investigate the morphological and electrophysiological mechanism of gate control for pain.We proposed that the regulation of peripheral A-fibers on C fibers was mediated by the GABAergic interneurons in the spinal cord.The cobra venom was injected to the sciatic nerve to establish a neuropathic pain model due to peripheral demyelination within A-fiber.The content of r aminobutyric acid(GABA)in spinal cord and cerebrospinal fluid was asssed by high performance liquid chromatography(HPLC).The microstructure of A-fiber,C-fiber and the GABAergic neurons in the spinal cord was observed by immunofluorescence staining.

摘要： to analysisBv adding different varieties of fiber, different filler to bituminous ration on the mixture of SMAthe infiltrationproperty of SMA mixture,ance of the SMA mixturedegrees, the shear strength and the stiffness modulus, in order to study the roadresult to reach a conclusion that the factors and the regular pattern of the performance of the SMA mixture.

摘要： 本发明属于生物工程技术领域，公开了一种构建促融质粒优化禽腺病毒Fiber‑2蛋白表达及纯化的方法。通过特定模板扩增Fiber‑2目的片段和PET‑32a‑T7‑TrxA载体片段，然后进行同源重组得到重组质粒PET‑32a‑T7‑TrxA‑Fiber2转化至Shuffle T7‑B感受态细胞进行Fiber‑2蛋白的表达，提高Fiber‑2蛋白的可溶性。并通过重组质粒上带有的his标签进行蛋白纯化并获得足够的蛋白量，为后续的亚单位疫苗的制备及免疫原性实验奠定基础。

摘要： 本发明公开了一种表达禽腺病毒4型截短Fiber2蛋白的重组新城疫病毒耐热疫苗株及其制备方法与应用，属疫苗基因工程领域。本发明采用RNA病毒的反向遗传操作技术，在转录质粒水平对NDV LaSota株进行基因改造，首先将其HN基因替换为耐热TS09‑C株的HN基因，再将禽腺病毒4型的截短Fiber2基因(961‑1440bp)插入至其P和M基因之间。将改造后的转录质粒转染宿主细胞，拯救并获得NDV rLS‑tFib2‑C株。该疫苗株具有显著的耐热特性，可极大降低对冷链系统的依赖，节省储运成本和提高疫苗热稳定性。该疫苗株表达的不是完整的Fiber2蛋白，只截取了其部分区域，针对性增强了疫苗的免疫保护效果。可用于制备禽安卡拉病、新城疫二联耐热活疫苗。

摘要： 本发明公开了一种重组Fiber2蛋白、其制备方法与应用。该蛋白的编码序列如SEQIDNO.1所示，用于制备重组Fiber2蛋白，步骤为：通过密码子优化合成Fiber2基因，在Fiber2的N端融合杜氏藻碳酸酐酶N‑半结构域的前11个氨基酸残基(VSEPHDYNYEK)和His

摘要： 本公开了一种基于RamanOpticalFiber的半开腔多波长随机拉曼光纤激光器，包括：1080nm泵浦激光器和半开腔随机拉曼光纤激光器。所述1080nm泵浦激光器包括：反射率99.9％的1080nm光纤布拉格光栅，976nm泵浦激光器，(2+1)×1泵浦合束器，NufernLMA‑YDF‑10/130‑M大模场双包层掺镱光纤；所述半开腔随机拉曼光纤激光器包括：1030‑1400nm宽波段光纤全反射镜，OFSRamanOpticalFiber。本发明利用长距离无源光纤中的瑞利散射和受激拉曼散射提供随机分布式反馈和增益放大，可同时输出1至7阶斯托克斯光，实现1.1‑1.6μm的宽光谱覆盖范围，且各阶斯托克斯光光谱强度差值不超过5dB，具有结构简单，输出波长灵活，输出激光时序稳定等特点，可广泛应用于测量、成像以及通信等各个领域。

摘要： 本发明公开了一种重组禽腺病毒4型(FAdV‑4)fiber2蛋白及其制备方法和应用，属于基因工程技术领域，其中所述重组蛋白为禽腺病毒4型fiber2蛋白去除了N端274个氨基酸残基后与禽腺病毒4型hexon蛋白第21‑55位氨基酸序列重组得到的蛋白，可用于制备禽腺病毒4型基因工程亚单位疫苗。本发明针对原核表达天然fiber2蛋白溶解性和免疫原性差的缺陷，对fiber2蛋白编码基因进行了系列改造，通过去除fiber2蛋白N端274个氨基酸残基的基因编码序列，并融合hexon蛋白的两个重要抗原表位的基因编码序列，使重组蛋白相比于改造前的fiber2蛋白，溶解性能和免疫原性均显著提高，因此可用于制备成一种品质可控，安全有效，能够预防高致病性FAdV‑4病毒感染的亚单位疫苗，免疫一次即可获得完全保护。

摘要： 本发明属于生物技术领域，主要涉及一种表达鸡4型腺病毒(FADV4)fiber2基因的重组鸡痘病毒转移载体及其构建方法和应用。所述载体以pMD19T‑Simple载体为基础，TA克隆位点插入FADV4fiber2基因、lacz基因，以及用于同源重组的鸡痘病毒的基因组复制非必须片段LTYB和RTYB。所述方法包括步骤如下：质粒pMD‑TYB；构建质粒pMD22；构建质粒pMD22‑lacz；构建中间载体pMD22‑TYB‑lacz；扩增FADV4 fiber2基因；构建重组鸡痘病毒转移载体pMD22‑TYB‑lacz‑F4；将转移载体pMD22‑TYB‑lacz‑F4与鸡痘病毒共转染鸡胚成纤维细胞，鉴定选出阳性，继续传代培养；鉴定重组鸡痘病毒的表达效果。本发明构建的重组鸡痘病毒转移载体为研制高效的表达鸡4型腺病毒的重组鸡痘病毒基因工程活载体疫苗奠定基础。

摘要： 本发明公开了fiber2蛋白及其重组蛋白在检测鸭血清中鸭2型腺病毒抗体方面的应用。首先基于fiber2蛋白获得了能与鸭2型腺病毒特异性抗体特异结合的重组DAdV‑2 fiber2蛋白，并进一步利用所述重组蛋白作为包被蛋白，建立了鸭血清样品中鸭2型腺病毒抗体特异性酶联免疫检测方法和检测试剂盒。该检测试剂盒灵敏度高、特异性强、稳定性好、能反映中和抗体水平，用于鸭血清中鸭2型腺病毒抗体的快速检测，具有操作简单、高特异性、高灵敏度、低成本的特点，能同时检测大量的样品，通过对鸭群血清样品的检测，可以实时监控鸭群的整体免疫效果及中和抗体水平，为该病的诊断和疫苗防控等提供了有力的基础和支持，具有很好的应用前景。

摘要： 本发明涉及基因工程疫苗技术领域，特别公开了一种I群4型禽腺病毒fiber‑2蛋白抗原及其制备基因工程亚单位疫苗的方法和应用。本发明在禽腺病毒FAdV‑4的fiber‑2基因前面添加非编码基因，并将其克隆到原核表达载体pET‑28a，转化至大肠杆菌构建工程菌，诱导工程菌表达获得可溶性fiber‑2蛋白。其中禽腺病毒fiber‑2抗原蛋白的氨基酸序列为SEQ ID NO.2，其编码的基因的核苷酸序列为SEQ ID NO.1，并且通过非编码基因调控获得高表达量的可溶性蛋白。本发明通过大肠杆菌表达禽腺病毒的fiber‑2蛋白，获得可溶性表达产物，制备亚单位疫苗，安全有效，可以预防禽I群4型腺病毒感染。

摘要： 本发明提供了一种重组鸭3型腺病毒fiber‑2基因在马克斯克鲁维酵母表达系统中的表达方法，该方法先将优化后的鸭3型腺病毒fiber‑2基因连接至表达质粒pUKD‑N115中，然后将连接产物转化至尿嘧啶缺失型马克斯克鲁维酵母菌TOP1中表达，最后获得了表达鸭3型腺病毒fiber‑2蛋白的重组酵母菌。本发明成功实现了重组质粒在马克斯克鲁维酵母中的高效表达，且含该表达产物的疫苗在对番鸭的腺病毒感染实验上取得了一定的保护效果。

摘要： 本发明提供了核苷酸序列、fiber2蛋白及表达方法、鸭3型腺病毒和鸭坦布苏病毒二联灭活疫苗。核苷酸序列，用于编码鸭3型腺病毒抗原性片段fiber2蛋白，其核苷酸序列如SEQ NO：2所示，鸭3型腺病毒抗原性片段fiber2蛋白的氨基酸序列如SEQ NO：3所示；将fiber2蛋白基因片段和pET‑32a载体片段通过T4DNA连接酶连接，得pET‑32a‑fiber2重组表达载体，然后转化至表达菌株E.coli BL‑21(DE3)感受态细胞培养，从培养物中获得所述的fiber2蛋白，鸭3型腺病毒和鸭坦布苏病毒二联灭活疫苗，其使用的抗原包鸭3型腺病毒抗原性片段fiber2蛋白和灭活的鸭坦布苏病毒；在二联灭活疫苗中两种抗原的体积比为1：1，鸭坦布苏病毒为鸭坦布苏病毒DF2株，其保藏编号为CCTCC NO：V201635。