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黏蛋白类

黏蛋白类的相关文献在2003年到2020年内共计109篇,主要集中在肿瘤学、内科学、基础医学 等领域,其中期刊论文109篇、专利文献214645篇;相关期刊56种,包括基础医学与临床、中华病理学杂志、国际检验医学杂志等; 黏蛋白类的相关文献由352位作者贡献,包括周向东、尤列·皮尔曼、李琪等。

黏蛋白类—发文量

期刊论文>

论文:109 占比:0.05%

专利文献>

论文:214645 占比:99.95%

总计:214754篇

黏蛋白类—发文趋势图

黏蛋白类

-研究学者

  • 周向东
  • 尤列·皮尔曼
  • 李琪
  • 维克多·科罗索夫
  • 汪荣泉
  • 彭志红
  • 何勇虹
  • 房殿春
  • 李哲夫
  • 万千雪
  • 期刊论文
  • 专利文献

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    • 刘亚男
    • 摘要: 目的:探讨热瞬时受体锚电位通道蛋白(TRPA1)在气道黏液高分泌中的作用及机制。方法:培养人支气管上皮16HBE细胞。用8%香烟提取物(CSE)处理人16HBE气道细胞后。分别给予TRPA1抗体阻断剂、NF-KB抑制剂(PDTC)进行干预,再予以香烟提取物刺激,以同体积磷酸盐缓冲液处理细胞为对照。酶联免疫吸附(ELISA)法检测各组中MUC5AC蛋白的相对表达量,逆转录-PCR检测MUC5ACmRNA的相对表达量,Western印迹法检测TRPA1水平,IkBa,磷酸化-IkBa、NF-kB蛋白的表达。结果:CSE刺激组细胞MUC5AC蛋白、MUC5ACmRNA、IkBa相对表达量(0.52±0.07、0.64±0.11、0.73±0.06、0.67±0.10、0.67±0.09)均显著高于空白对照组(0.26±0.10、0.28±0.06、0.30±0.05、0.30±0.08、0.36±0.08)、TRPA1抗体组(0.30±0.12、0.29±0.09、0.34±0.06、0.47±0.19、0.39±0.13)和PDTC组(0.38±0.06、0.31土0.14、0.39±0.04、0.44±0.12、0.48±0.11),均P<0.05)。结论:TRPA1可能通过IkBa/NF-kB信号通路上调气道16HBE细胞中MUC5AC的表达。
    • 熊艺琳; 宋军; 侯晓华
    • 摘要: 肠道黏液屏障是由黏蛋白、水、抗菌肽等组成的凝胶网状结构,是肠腔抵御病原体的第一道防线,也是共生菌群生存、繁殖的重要环境.黏蛋白主要由肠道杯状细胞合成、分泌,在不同肠段形成结构不同的黏液层,其过程受多种因素调控.目前越来越多研究表明,黏液屏障除与肠道感染、炎症性肠病、肠道肿瘤等各种肠道疾病密切相关外,还参与某些肠外疾病如急性胰腺炎、非酒精性脂肪性肝病的发生、发展,未来可能成为各种相关疾病治疗的新靶点.
    • 谭雅芹; 彭志红
    • 摘要: 上皮-间质转化(EMT)是一种复杂的转化过程,在癌细胞的侵袭过程中发挥重要作用.癌细胞形态变化伴随多种细胞改变过程,如细胞间黏附改变、细胞基质、降解、上皮标志物、间充质标志物改变等.黏蛋白(MUC)广泛分布于各种组织器官的上皮细胞中,在EMT过程中起重要作用.同时,MUC还可介导信号转导和细胞黏附、参与肿瘤的浸润、转移等.本文就EMT和MUC在肿瘤研究中的进展作一综述.
    • 李琪; 周向东; 曾曼; 钟有清; 维克多·科罗索夫; 尤列·皮尔曼
    • 摘要: 目的:了解皮层肌动结合蛋白(cortical actin-binding protein,又称cortactin)在人气道上皮细胞黏蛋白(mucin,MUC)5AC分泌中的作用及不同磷酸化位点对其的影响.方法:培养人气道上皮细胞HBE16,以皮层肌动结合蛋白不同磷酸化位点突变载体转染细胞,并分为空白对照组、剪应力刺激组、剪应力+增强绿色荧光蛋白质粒(enhanced green fluorescent protein plasmid,pEGFP)-N1组、剪应力+pEGFP-N1-cortactin (Cort)组、剪应力+pEGFP-N 1-Cort-Y421A组、剪应力+pEGFP-N 1-Cort-Y470A组及剪应力+pEGFP-N1-Cort-Y486A组,剪应力设定为4 dynes/cm2.采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)、real-time PCR法测定转染前后各组细胞及培养上清中MUC5AC蛋白和mRN水平,Western印迹检测各组皮层肌动结合蛋白及磷酸化皮层肌动结合蛋白含量;异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的鬼笔环肽染色观察丝状肌动蛋白(filamentous actin,F-actin)的分布情况.结果:4 dynes/cm2剪应力刺激30 min后,细胞中磷酸化皮层肌动结合蛋白表达增加,在2h时表达水平最高;剪应力刺激2h后,MUC5AC mRN表达增强,细胞及培养上清中MUC5AC的含量与空白对照组相比,各组均显著增加(均P<0.05);与剪应力刺激组相比,剪应力+pEGFP-N 1-cortactin(Cort)组的细胞培养上清液中MUC5AC蛋白含量显著增加,F-actin在胞膜的分布也明显增多(均P<0.05),而胞质内MUC5AC蛋白及MUC5AC mRN水平变化不大.与剪应力+pEGFP-N1-Cort组相比,剪应力+pEGFP-N1-Cort-Y421A组、剪应力+pEGFP-N 1-Cort-Y470A组培养上清液中的MUC5AC蛋白含量有明显降低,F-actin在胞膜的聚集也有所减少(均P<0.05),而剪应力+pEGFP-N1-Cort-Y486A中MUC5AC含量变化不明显(P>0.05).结论:皮层肌动结合蛋白参与了剪应力诱导产生的人气道上皮细胞MUC5AC蛋白分泌,Tyr421和Tyr470位点磷酸化在其中可能起重要的作用.
    • 陈璀璀; 李永磊; 芦二永
    • 摘要: 目的:探讨通窍鼻炎方对慢性鼻窦炎患者体内Foxa2、黏蛋白M UC5AC、HIF-1α水平的影响性.方法:选取116例慢性鼻窦炎患者作为研究对象,按入院先后顺序平均分成两组,均为58例.对照组常规治疗,研究组在对照组基础上加用通窍鼻炎方治疗,观察不同方法治疗后在临床症状、疗效、实验室指标和生活质量变化情况.结果:两组治疗前流脓、鼻塞、头昏头痛、嗅觉减退、黏膜充血肿胀积分和炎症指标IL-β、IL-5、TNF-α以及Foxa2、黏蛋白MUC5AC、HIF-1α比较差异不显著(P>0.05);对照组治疗前末在Foxa2、黏蛋白M UC5AC、HIF-1α水平比较差异无统计学意义(P>0.05);余治疗末较治疗前以上指标两组均显著下降,差异有统计学意义(P<0.05),治疗末研究组以上指标较对照组差异显著(P<0.05).疗效上,对照组治愈率22.41%、总有效率81.03%;研究组治愈率36.21%、总有效率93.1%,研究组显著优于对照组,比较差异有统计学意义(P<0.05).结论:通窍鼻炎方能抑制慢性鼻窦炎患者体内Foxa2、黏蛋白M UC5AC、HIF-1α表达,从而提高疗效,改善临床症状.
    • 王巧兴; 吴琦; 孙昕; 李敏敏; 宋丽佳; 刘会金; 陈怀永; 李红蔚
    • 摘要: 目的 通过研究叶内型肺隔离征(ILS)中基底干细胞的增殖情况探讨ILS的发病机制.方法 分别收集4例ILS患者的肺组织和4例健康对照肺组织.采用HE染色比较2组肺组织镜下病理改变,PAS染色比较2组肺组织气道杯状上皮细胞比例,免疫荧光染色及Real-time PCR法比较2组黏蛋白MUC5AC和MUC5B的分泌及表达,免疫荧光染色比较纤毛细胞的分布及基底细胞的增殖情况.结果 ILS组肺组织中充满炎性细胞并有气道上皮的损伤、气道杯状上皮细胞明显增殖、MUC5AC和MUC5B呈高分泌状态.角蛋白5(KRT5)在2组中差异无统计学意义,但增殖抗原KI67阳性(KI67+)/KRT5+细胞在ILS组明显减少.结论 通过增加气道上皮的增殖来修复并维持气道完整性为未来非外科治疗ILS提供了一个新方向.%Objective To investigate the proliferation potential of the basal stem cells in intralobar pulmonary sequestration syndrome (ILS) for revealing the pathogenesis of ILS. Methods In this study, lung tissue samples were collected from healthy control subjects(n=4)and abnormal lung lobes of ILS patients(n=4).The pathological changes were compared by HE staining between the two groups.The proportion of goblet cells was compared by PAS staining between the two groups.The expression and secretion of MUC5AC and MUC5B were compared by immunofluorescence staining and real-time PCR between the two groups. The distribution of ciliated cells and the proliferation of basal cells were compared by immunofluorescence staining between the two groups.Results The abnormal lobe of ILS group was filled with inflammatory cells, and the airway epithelium was disrupted. The airway goblet cells of ILS were obviously hyperplastic. The mucin proteins of MUC5AC and MUC5B were hypersecretion in the abnormal lobe of ILS patients.KRT5-positive basal stem cells proliferated only slightly in ILS patients, although there was no significant difference in KRT5 expression between two groups. Conclusion These data suggest that the pathogenesis of ILS may be associated with defects in basal stem cell function. Restoring airway integrity by targeting epithelial regeneration can be a future non-surgical treatment for patients with ILS.
    • 邓磊; 何松; 赵晓莉
    • 摘要: 目的 探讨血清MUC1(sMUC1)在溃疡性结肠炎、结直肠癌患者及健康对照人群中的表达水平,分析各组间的表达差异,了解sMUC1表达的临床意义.方法 选取2015年9月至2016年10月该院确诊的溃疡性结肠炎患者血清31例,结直肠癌患者血清31例,将结直肠癌组根据淋巴结及脏器转移情况分为转移组和未转移组;随机采集在该院体检中心健康体检者血清31例为对照组.采集入组病例临床检查资料,并使用酶联免疫吸附试验(ELISA)方法检测所有入组对象的sMUC1水平,分析组间差异.结果 结直肠癌组和溃疡性结肠炎组的sMUC1表达水平[(9.98±7.75)、(8.64±6.09)U/mL]均明显高于对照组[(6.06±3.49)U/mL],差异均有统计学意义(P=0.014、0.047).结直肠癌组内分析,其中转移组sMUC1表达水平[(13.80±9.69)U/mL]较高,与未转移组[(6.82±3.58)U/mL]比较,差异有统计学意义(P=0.010).结论 在结直肠癌及溃疡性结肠炎患者中,sMUC1表达水平明显升高,且肿瘤转移患者sMUC1水平升高更为明显,提示MUC1的表达与炎症及肿瘤的发生发展有一定相关性,检查sMUC1水平对溃疡性结肠炎和结直肠癌的发生风险及肿瘤转移具有一定预判价值.
    • 张重捷; 邹奇; 陈杰; 李春生
    • 摘要: Purpose To prepare superparamagnetic iron oxide nanoparticles (SPION) probe targeted and modified by MUC1 murin (MUC1) in order to explore its MRI characteristics in pancreatic cancer transplantation model.Materials and Methods Chemical conjugate method was adopted for coupled response of MUC1 and SPION to construct targeted probe and tested its basic physical properties,including water and diameter,surface charge and MR signal measuring.Meanwhile,nude mice model of pancreatic cancer transplant subcutaneous sarcoma was set up to study imaging effect inside the nude mice.Transplant sarcoma specimen was taken and immunohistochemical and Western blot were adopted to measure MUC1 expression.Results Partial size of the prepared particle probe was approximately 63.5 nm and surface charge was about 10.2 mV.The probe solution could obviously decrease MR transverse relaxation time (T2 value).In vitro experiment,MUC 1 could selectively gather on nude mice transplant sarcoma model could greatly lower T2 signal intensity.Conclusion Prepared probe has small partial size,superparamagnetic and other advantages.It can realize combination with pancreatic cancer tissue specificity and provide reliable in vivo iconology in early stage for disease diagnosis through vitro imaging.%目的 制备MUC1黏蛋白1(MUC1)靶向修饰的探针超顺磁纳米颗粒(SPION),探讨其在胰腺癌移植模型中的MRI特点.材料与方法 采用化学共轭法将MUC1与SPION耦联,构建靶向探针,检测其基本物理特性,包括水和直径、表面电荷及MR信号测定;同时建立胰腺癌皮下移植瘤裸鼠模型,研究在裸鼠体内的成像效果.取移植瘤标本,采用免疫组化及蛋白质印迹法测定MUC1的表达.结果 制备的分子探针颗粒大小约为63.5 nm,表面电荷约为10.2 mV,该探针溶液能显著降低MR横向弛豫时间(T2值).在体内实验中,MUC1可以选择性地积聚在裸鼠移植瘤模型上,并能显著降低T2信号强度.结论 制备的探针具有粒径小、超顺磁性等优点,并能实现与胰腺癌组织特异性结合,通过体内成像,为疾病的诊断提供早期可靠的活体影像学资料.
    • 陈昕; 汪希鹏
    • 摘要: MUC16,又名CA125,是一种表达于各类上皮细胞表面的高分子量糖蛋白,主要发挥保护和修复上皮的作用。MUC16是早期诊断上皮性卵巢癌的重要肿瘤指标,广泛应用于临床。近来研究发现,MUC16的异常表达与卵巢癌的不良预后和发生发展密切相关。MUC16可以通过与间皮素结合促进卵巢癌远处转移,可以通过抑制肿瘤细胞和免疫细胞形成免疫突触帮助卵巢癌细胞实现免疫逃逸。MUC16还可以通过在卵巢癌中的过表达来影响肿瘤细胞的上皮间质转化(EMT)、增殖、迁移和转移,促进卵巢癌的发生发展。尽管有关MUC16与卵巢癌的研究逐渐取得进展,但MUC16的生化结构及其在卵巢癌中具体作用机制仍处于研究阶段。综述MUC16的生化结构及其在卵巢癌细胞的免疫逃逸和卵巢癌增殖、迁移、侵袭和转移中的研究进展,旨在为卵巢癌的治疗提供新的靶点。%MUC16, also named CA125, is a kind of high molecular weight glycoprotein expressed by various epithelial cells. It participates in protecting and repairing epithelium. MUC16 is one of the most important diagnostic biomarkers for early stage epithelial ovarian cancer. Recent studies have found that the abnormal expression of MUC16 in ovarian cancer is closely related to tumorigenesis and poor prognosis of ovarian cancer. MUC16 can bind to mesothelin to promote ovarian cancer distant metastasis. It can inhibit immune synapse formation between immune cells and tumor cells to help ovarian cancer cells evade immune attack. The overexpression of MUC16 can also affect EMT, proliferation, migration and metastasis of tumor cells to enhance ovarian cancer progression. Although much progress is being made in understanding the relationship between MUC16 and ovarian cancer, the biochemical structure of MUC16 and its specific mechanism in ovarian cancer are still unclear. This review makes a summary on the biochemical structure of MUC16 and its role in immuno-escape, proliferation, migration, invasion and metastasis of ovarian cancer, to provide new targets for the treatment of ovarian cancer.
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