摘要：
An efficient ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous analysis of residues of 8 antiviral drugs (arbidol,amantadine,rimantadine,famciclovir,peramivir,imiquimod,oseltamivir,and lamivudine) in chicken meat and liver and pork meat and liver.Samples were extracted with 1％ formic acid-acetonitrile,and the extract was subjected to chromatographic separation on MCX solidphase extraction column.Quantification of the analytes was achieved by UPLC-MS/MS using the internal standard method.The limit of detection (LOD) and limit of quantification (LOQ) of the method were lower than 1.0 and 3.0 μg/kg for all the antiviral drugs,respectively.The average recoveries of 8 antiviral drugs in blank chicken meat,pork meat,chicken liver and pork liver at spiked levels of 5.0,10.0,and 20.0 μg/kg ranged from 70％ to 120％,with relative standard deviation (RSD) of less than 10％.The method had the advantages of small matrix interference,high sensitivity and good recovery.Therefore,it can be used for the detection of antiviral drugs residues in animal-derived food.%建立同时测定鸡肉、猪肉、鸡肝和猪肝中阿比朵尔、金刚烷胺、金刚乙胺、泛昔洛韦、帕拉米韦、咪喹莫特、奥司他韦、拉米夫定8种抗病毒药物残留的超高效液相色谱-串联质谱的检测方法.样品采用乙腈-0.1％甲酸溶液(9∶1,V/V)经一次性振荡提取,用MCX固相萃取柱净化,内标法定量.结果表明:8种抗病毒药物的检出限均低于1.0μg/kg,定量限均低于3.0 μg/kg,空白鸡肉、鸡肝、猪肉、猪肝中分别添加5.0、10.0 μg/kg和20.0 μμg/kg,8种抗病毒药物的回收率均在70％～120％之间.该方法基质干扰小、灵敏度高、回收率好,可用于日常动物性样品中抗病毒类药物的残留检测.
