摘要：
According to the potential carryover of long residual pesticide's residual to rotational crops,takingHaloxyfop-R-methyl as a model pollutant, three bacterial strains were isolated by selective enrichment from soil exposed to long residual herbicides, then the cultural characteristics and biochemical features of the three strains were determined and identified as HM1 ,HM2 and HM3,HM1 and HM2 were identified as Bacillus sp. , while HM3 was identified as Methyloeoccus sp. Then the effect of sugar content, temperature, pH value and initial concentration of haloxyfop-R-methyl on the growth of the three strains were studied,it was found out that the optimal sugar content for HM1 and HM3 was 0％,and HM2 was 0.5％; the optimal temperature range for HM3 and HM1 was 20 °C to 37 °C ,and HM2 was 25 °C to 41 °C; the optimal range of pH for HM3 was 6.0 to 9.0 ,that for HM1 and HM2 was 6.0 to 8.0; the optimal initial concentration of Haloxyfop-R-methyl for HM3 was 200 mg/L,that for HM1 and HM2 was 100 mg/L.%针对海南地区长残效除草剂残留对后茬产生药害问题,以高效盖草能作为研究对象,采用富集培养的方法从长期施用长残效除草剂的土壤中分离到3株细菌,经过对这3株降解菌的培养特性及生理生化特征的测定试验,经传统鉴定方法对3种菌种进行鉴定,结果显示,HM1和HM2同为芽孢杆菌属(Bacillus sp.),HM3为甲基球菌属(Methyloeoccus sp.).并分别研究了培养含糖量、温度、pH和高效盖草能初始浓度对分离出的3株降解菌生长的影响,结果表明,HM1和HM3的最适含糖量是0.HM2的最适含糖量为0.5%;HM1和HM3的最适温度范围相同,为20～37°C,HM2的最造温度为25～41°C;HM1、HM2和HM3的最适生长温度分别是35、30和30°C;HM3的生长对酸碱度要求不严格,最适生长pH范围为6.0～9.0.HM1适宜在中性偏酸的条件下生长,而HM2则适宜在中性偏碱的条件下生长,其最适生长pH均为6.0～8.0;HM1和HM2生长的最适高效盖草能浓度是100 mg/L,HM3生长的最适高效盖草能浓度为200mg/L.
