摘要：
为评估草莓的微生物污染情况,在2016年3—4月份对浙江地区基地温棚和市售草莓进行随机抽样,分析草莓中菌落总数、大肠菌群、霉菌,并运用全自动微生物分析系统(VITEK)和分子生物学技术,分别对分离的革兰氏阴性细菌和霉菌进行了鉴定.结果表明:温棚草莓菌落总数为120~48600 cfu·g-1,大肠菌群为3~1100 MPN·g-1,霉菌为0~2900 cfu·g-1;市场草莓菌落总数为1900~36900 cfu·g-1,大肠菌群为15~1100 MPN·g-1,霉菌为200~6100 cfu·g-1;同时鉴定出大肠埃希菌(Escherichia coli)、阴沟肠杆菌(En-terobacter cloacae)、河生肠杆菌(Enterobacter.amnigenus)、植生拉乌尔菌(Raoultella planticola)等19株革兰氏阴性细菌和毛霉属(Mucor)、镰刀菌属(Fusarium)等5株霉菌,所检出的革兰氏阴性细菌和霉菌多数具有较强的致病性.研究结果为草莓微生物数量的控制和开发杀菌抑菌技术提供了参考.%In order to evaluate the status of microbial contamination in strawberry, hygienical microbiology was exam-ined from strawberry which sampled in greenhouse and market of Zhejiang Province during March to April in 2016 year according to the food hygiene standard of People Republic of China. Then bacteria counting, enumeration of co-liforms and mold detection were carried out. The bacteria and mold which were isolated from the samples were identi-fied by Vitek Auto Microbic System and Molecular biological methods, respectively. The results indicated that the number of bacteria, coliforms and mold of strawberry in greenhouse ranged from 120 to 48600 cfu·g-1 , from 3 to~1100 MPN·g-1 , from 0 to 2900 cfu·g-1 , respectively. And the number of bacteria, coliforms and mold of straw-berry in market ranged from 1900 to 36900 cfu·g-1 , from 15 to 1100 MPN·g-1 , from 200 to 6100 cfu·g-1 , re-spectively. Meanwhile, 19 gram negative bacteria species such as Escherichia coli, Enterobacter cloacae, Enterobact-er amnigenus, Raoultella planticola and 5 mold species such as Mucor and Fusarium were identified. Most of the i-dentified bacteria and mold had serious pathogenicity. The experimental results would be helpful to control the num-ber of microorganisms and develop new sterile technology for strawberry.
