摘要:
Objective To investigate the results difference of the indirect immunofluorescence (IIF) and immunoblotting (LIA) for detecting ant-inuclear antibodies (ANA) and clinical value .Methods One hundred and forty-six ANA detection specimens of IIF negative and LIA positive were collected and performed the detection of HBV antibodies and anti-HCV antibodies .Results (1) In 146 specimens of IIF (-)LIA (+ ) ,the positive specimen numbers of single anti-Ro52 antibody ,anti-SSA antibody and anti-AMA-M2 antibody were 69 cases ,42 cases and 15 cases respectively ,which of anti-RNP antibody ,anti-PCNA antibody and PM-Scl antibody were 3 cases ,5 cases and 2 cases respectively ,the combined 2-item positive was in 8 cases .(2)Among positive specimens of single anti-Ro52 antibody ,HBsAg(+ ) ,anti-HBe(+ ) ,anti-HBc(+ ) model and HBsAg(+ ) ,HBeAg(+ ) ,HBcAb(+ ) model of hepatitis B ,and hepatitis C were 51 cases ,4 cases and 7 cases respectively ,7 cases were non-hepatitis patients .(3) Among positive specimens of single ant-SSA antibody ,6 cases were hepatitis B patients and 36 cases were the patients with non-hepatitis B .Conclu-sion Anti-Ro52 antibody and anti-SSA antibody are easier to be missed by the IIF detection .Anti-Ro52 antibody positive has a cer-tain relation with small three positive of hepatitis B .%目的 探讨间接免疫荧光法(IIF)和免疫印迹法(L IA)检测抗核抗体(A N A)的结果差异及临床价值.方法 收集146例ANA检测IIF(-)与LIA(+)的标本,并进行乙型肝炎病毒(HBV)抗原、抗体和丙型肝炎病毒(HCV)抗体的检测.结果 (1)在146例IIF(-)LIA(+)的标本中,单独出现抗Ro52抗体、抗SS-A、抗AMA-M2抗体阳性的标本数分别为69例、42例、15例,抗RNP抗体、抗PCNA抗体、PM-Scl抗体阳性的标本数分别为3例、5例、2例,有两项阳性的共8例.(2)单Ro52抗体阳性标本中,乙肝小三阳:HBsAg(+)、HBeAb(+)、HBcAb(+),乙肝大三阳:HBsAg(+)、HBeAg(+)、HBcAb(+)及丙型肝炎患者患者例数分别为51例、4例、7例,非肝炎患者7例.(3)单SSA抗体阳性标本中,乙型肝炎患者6例,非乙肝的患者36例.结论 IIF检测ANA容易漏检Ro52抗体、SSA抗体,Ro52抗体与乙肝小三阳有一定关系.