摘要：
目的:建立UFLC-QTRAP-MS/MS的方法,同时测定不同产地绞股蓝及其商品药材中10个化合物(胸苷、尿苷、腺苷、肌苷、2'-脱氧鸟苷、鸟苷、腺嘌呤、次黄嘌呤、尿嘧啶、鸟嘌呤)的含量.方法:采用XBridge Amide色谱柱(2.1 mm×100 mm,3.5 μm),以0.2％甲酸水溶液(A)-0.2％甲酸乙腈溶液(B)为流动相,0.6 mL· min-1流速梯度洗脱,正离子(ESI+)模式下多反应监测(MRM)检测.结果:尿嘧啶、胸苷、尿苷、次黄嘌呤、肌苷、2'-脱氧鸟苷、腺苷、鸟苷、腺嘌呤、鸟嘌呤的线性范围分别为1.97～3 936.00、2.00～200.00、30.48～3 048.00、19.84～1 984.00、10.16～1 016.00、4.95～990.00、19.72～1 972.00、14.70～2 940.00、1.96～196.00、14.76～1 476.00 ng· mL-1(r＞ 0.997 3),平均加样回收率为99.3％～101.3％,含量分别为181.54～1 604.95、0.00～216.45、63.65～1 093.17、3.48～555.12、0.07～215.66、0.00～171.65、0.00～338.63、24.67～966.08、0.37～140.07、14.89～830.79 μg·g-1,绞股蓝中以尿嘧啶、尿苷、鸟苷、鸟嘌呤含量较高;不同产地及商品药材中10个成分含量有所差异.结论:该方法为绞股蓝药材内在质量的综合评价提供新的科学依据.%Objective:To establish a method for simultaneous determination of 10 components (thymidine,uridine,adenosine,inosine,2'-deoxyguanosine,guanosine,adenine,hypoxanthine,uracil and guanine) in Gynostemma pentaphyllum from different habitats and commercial sources by ultra-fast liquid chromatography tandem combined with triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS).Methods:The analysis was carried out on an XBridge Amide column (2.1 mm × 100 mm,3.5 μm)which was eluted by a mobile phase of 0.2％ formic acid-0.2％ formic acid in aeetonitrile at a flow rate of 0.6 mL· min-1.The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode.Results:Uracil,thymidine,uridine,hypoxanthine,inosine,2'-deoxyguanosine,adenosine,guanosine,adenine and guanine showed good linearity(r ＞ 0.997 3)in therange of 1.97-3 936.00 ng· mL-1,2.00-200.00 ng· mL-1,30.48-3 048.00 ng· mL-1,19.84-1 984.00 ng· mL-1,10.16-1 016.00 ng· mL-1,4.95-990.00 ng· mL-1,19.72-1 972.00 ng· mL-1,14.70-2 940.00 ng· mL-1,1.96-196.00 ng· mL-1 and 14.76-1 476.00 ng· mL-1,respectively.The average recoveries of the ten components were between 99.3％ and 101.3％.The contents of ten components were in the range of 181.54-1 604.95 μg· g-1,0.00-216.45 μg· g-1,63.65-1 093.17 μg· g-1,3.48-555.12 μg· g-1,0.07-215.66 μg· g-1,0.00-171.6 5 μg· g-1,0.00-338.63 μg· g-1,24.67-966.08 μg· g-1,0.37-140.07 μg· g-1 and 14.89-830.79 μg· g-1,respectively.The contents of uracil,uridine,guanosine and guanine were higher in Gynostemma pentaphyllum.The contents of ten components varied by habitats and commercial sources.Conclusion:The established method provides scientific basis for the comprehensive quality evaluation of Gynostemma pentaphyllum.
