背根节

摘要： 目的 观察背根节脉冲射频治疗神经根型颈椎病的疗效及安全性.方法 60例神经根型颈椎病患者,随机分为A、B两组,每组30例:A组于数字减影血管造影机(DSA)引导下行选择性神经根阻滞;B组在DSA引导下行背根节脉冲射频治疗.观察两组术前和术后1、7 d及术后1、3、6个月时患者的视觉模拟评分(VAS),术后1、3、6个月随访Odom评分的优良率以及手术相关并发症.结果 两组患者术后疼痛VAS评分均迅速降低,同术前相比差异均有统计学意义(P<0.05);B组患者VAS评分术后3、6个月随访时仍维持在较低水平,与A组相比,差异有统计学意义(P<0.05);同时,两组Odom评分优良率在术后3、6个月随访时差异有统计学意义(P<0.05);两组均未见严重手术相关并发症.结论 背根节脉冲射频能快速减轻神经根型颈椎病患者的症状,并且能维持较长时间,未见明显手术并发症,是治疗神经根型颈椎病的一种快速、安全、有效的方法.

摘要： 目的:探讨坐骨神经损伤后,大鼠背根节中感觉神经元的表达变化,为外周神经损伤后神经病理性疼痛的治疗提供理论依据.方法:将SD大鼠分成正常组(3只)和实验组(36只),将实验组大鼠进行坐骨神经切断,分别在术后7天、30天、90天取背根节(L3-L6),进行免疫荧光化学染色,检测神经元的表达变化.结果:成年SD大鼠背根节的感觉神经元数量在坐骨神经神经损伤后7-30天,比正常组明显减低.术后90天检测背根节神经元数目有较大增加.结论:坐骨神经损伤可导致背根节感觉神经元死亡,数目减少,但损伤一定时间之后又有神经元数目增多的现象.

摘要： Objective: To observe the effect of paeoniflorin on pain behavior and the expression of Sirt1 in spinal dorsal horn and dorsal root ganglion in rats with chronic constriction injury. Methods: Forty-eight male SD rats were randomly divided into sham operation group, negative contract group (SC group), CCI group and paeoniflorin group. Heat shrinkable foot reflex latency (TWL) and mechanical paw withdrawal reflex threshold (MWT) were detected for rats in each group at 1 day before operation and postoperative 3, 7 and 14 days. Rats were sacrificed at 3, 7 and 14 days after surgery then L4-5 segment of spinal cord and dorsal root section orga-nization was taken, and real-time PCR was used for expression of Sirt1, Western blot was used in the same time to detect the change of Sirt1 protein level. Results: At 3, 7 and 14 days after surgery, TWL and MWT of CCI group decreased significantly compared with sham group (P<0.05), and achieved the minimum value at the third day. Real-time PCR showed that the expression of Sirt1 of CCI group of spinal dorsal horn and dorsal root gan-glia were significantly decreased at the 7th day and 14th day after surgery compared with sham group (P<0.05);the expression of Sirt1 of paeoniflorin group was upregulated compared with SC group (P<0.05); the results of Western blot also showed that the level of Sirt1 protein in the dorsal horn and dorsal root ganglion were gradually increased with time, and there was difference between the Sirt1 group and the SC group at 14th day. Conclusion:Paeoniflorin can alleviate by pain neuropathic disease caused, and the up regulation of the expression of Sirt1 protein in dorsal horn and dorsal root ganglion of the spinal cord is a possible mechanism.%目的:观察芍药苷对慢性压迫性损伤(CCI)大鼠的疼痛行为和Sirt1在脊髓背角及背根节的表达变化.方法:选择雄性SD大鼠48只,按照设计随机分为假手术组(Sham组)、阴性对照组(SC组)、CCI组和芍药苷组.每组在术前和术后3、7和14 d检测大鼠的后足热缩足反射潜伏期(TWL)和机械缩足反射阈值(MWT);选择在术后3、7和14 d的相同时间处死大鼠,取大鼠L4~5节段的脊髓和背根节组织备用,用real-time PCR检测Sirt1在组织内表达水平,采用Western blot法检测组织内Sirt1蛋白表达变化.结果:术后3、7和14 d,与Sham组比,CCI组TWL与MWT值均显著降低(P<0.05),并且在术后3 d达到最小值;real-time PCR结果显示,与Sham组相比,术后7 d和14 d,CCI组脊髓背角及背根节的Sirt1基因表达显著降低(P<0.05),与SC组对比,芍药苷组脊髓背角及背根节的Sirt1基因的表达上调(P<0.05);Western blot结果表明芍药苷组脊髓背角及背根节的Sirt1蛋白的表达随时间逐步升高,但在14 d时仍低于SC组(P<0.05).结论:芍药苷能够缓解神经病理性疾病引发的疼痛,其中上调脊髓背角和背根节Sirt1的表达是其可能机制.

摘要： 目的 观察鞘内注射右美托咪定对背根节慢性受压痛大鼠(CCD)脊髓CaMKⅡ表达的影响,探讨右美托咪定-CaMKⅡ通路在大鼠背根节慢性受压痛中的作用.方法 鞘内置管成功的雄性SD大鼠48只,随机分为6组,每组48只.分别是正常对照组(C组);模型组(CCD组);生理盐水组(NS组);右美托咪定2μg/kg组(Dex2);右美托咪定4 μg/kg组(Dex4);右美托咪定8 μg/kg组(Dex8).分别于鞘内置管前(T1),制备CCD模型前(T2)、CCD模型制备后5d鞘内给药前(T3)、鞘内给药后30 min(T4)、60 min(T5)、120 min(T6)、240 min (T7)检测大鼠机械缩腿阈值(MWT)和热缩足潜伏期(TWL).并于鞘内给药后240 min,各组取4只大鼠处死,取脊髓腰膨大,采用免疫印迹法检测CaMKⅡ的表达.结果 与C组比较,CCD组大鼠在T3～T7各时点MWT及TWL均显著降低,CCD组大鼠表现为明显的机械痛敏感和热痛敏感.NS组大鼠鞘内注射NS后对大鼠MWT及TWL无影响,鞘内注射不同剂量的右美托咪定则可显著提高CCD大鼠MWT和TWL.与C组(0.24±0.04)比较,CCD组(0.59±0.09)、NS组(0.61±0.08)大鼠CaMKⅡ蛋白表达均显著增加;鞘内注射不同剂量的右美托咪定后,大鼠脊髓CaMKⅡ蛋白表达(0.45±0.06、0.39±0.05、0.36±0.06)增加幅度显著降低.结论 鞘内注射右美托咪定可减轻CCD大鼠机械痛敏感和热痛敏感,抑制CCD大鼠脊髓CaMKⅡ蛋白表达.

摘要： Objective: To observe the effects of calcium chennel Cav3.2 on CaMK Ⅱ expression in the spinal cord of the rats following chronic dorsal root ganglia compression, we investigated the roles of Cav3.2-CaMK Ⅱ pathways in neuropathic pain. Methods: 60 male Sprague Dawley rats weighing 250 ± 20 g were randomly divided into 5 groups of 12 rats each , 8 rats for behavior experiment and 4 rats for western blot respectivly: Control group (group C); Model group (CCD group); Saline group (NS group); Cav3.2 missense oligodeoxynucleotides group (MS-Cav3.2 group); Cav3.2-antisense oligodeoxynucleotides group (AS-Cav3.2 group). Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of the rats were measured before (Tl) and 3 days after (T2) implantation of intrathecal catheters, 1 day (T3), 4 d (T4) after drug or NS intrathecal injection, 5 d (T5), 10 d (T6), 15 drn(T7) after CCD model preparation. Four rats in every group were executed and spinal cord were isolated to detect Cav3.2 and CaMK Ⅱexpression with western blot after 5 days of CCD model preparation. Results: Compared with C group, MWT and TWL of the rats in CCD group statistically decreased at T5, T6 and T7. There were no significant effects on the MWT or TWL after intrathecal injection of Saline or Cav3.2 miss-sense oligodeoxynucleotides. However, MWT and TWL of the rats significantly increased after anti-sense oligodeoxynucleotides injection. Cav3.2 and CaMK Ⅱ proteins were detected in the spinal cord of the rats in C group. Moreover, Cav3.2 and CaMKⅡ proteins were upregulated 5 days after CCD model preparation. There were no significant effects on the Cav3.2 and CaMK Ⅱ proteins expression after saline and Cav3.2 miss-sense oligodeoxynucleotides intrathecal injection. However, Cav3.2 and CaMKⅡ proteins expression were significantly decreased after Cav3.2 anti-sense oligodeoxynucleotides injection. Conclusion: Inhibition of Cav3.2 expression can reduce CaMK Ⅱ expression in the spinal cord of the rats with chronic dorsal root ganglia compression.%目的:观察Cav3.2通道对背根节慢性压迫痛大鼠脊髓CaMKⅡ表达的影响,探讨Cav3.2-CaMKⅡ通路在神经病理性疼痛中的作用.方法雄性SD大鼠60只,体重250±20 g,随机分为5组,每组12只(其中行为学实验8只,免疫印迹实验4只).分别是正常对照组(C组)；模型组(CCD组)；生理盐水组(NS组)；错义寡聚核苷酸组(MS-Cav3.2组)；反义寡聚核苷酸组(AS-Cav3.2组).分别于鞘内置管前(T1),鞘内置管后3 d (T2),鞘内给药后ld(T3)、4d(T4),CCD模型制备后5d (T5)、10d(T6)、15d(T7)检测大鼠机械缩腿阈值(mechanical withdrawal threshold,MWT)和热缩足潜伏期(thermal withdrawal latency,TWL).并于CCD模型制备后5d,各组取4只大鼠处死,取脊髓腰膨大,采用免疫印迹法检测Cav3.2及CaMKⅡ的表达.结果:与C组比较,CCD组大鼠在模型制备后第5d、10d、15d时MWT及TWL均显著降低.鞘内注射NS和Cav3.2错义寡聚核苷酸对CCD大鼠MWT及TWL无影响,鞘内注射Cav3.2反义寡聚核苷酸可增加CCD大鼠MWT和TWL,减轻大鼠机械痛敏和热痛敏.C组大鼠脊髓Cav3.2和CaMKⅡ蛋白均有表达.大鼠在制备CCD模型后5 d Cav3.2及CaMKⅡ蛋白表达均显著增加.鞘内注射NS及Cav3.2错义寡聚核苷酸对Cav3.2及CaMKⅡ蛋白表达无影响,鞘内注射Cav3.2反义寡聚核苷酸则可显著抑制Cav3.2及CaMKⅡ蛋白的表达.结论:抑制脊髓Cav3.2通道的表达可降低慢性背根节压迫痛大鼠脊髓CaMKⅡ的表达.

摘要： In order to remove the noise from the discharging signal of dorsal root ganglion(DRG)neurons for further analysis,the wavelet filtering method was used.First the signal with noise was decomposed through the harr wavelet,and then the GCV algorithm was used to determine?the optimal threshold based on soft-threshold method,at last the signal was reconstructed.It demonstrates that the method using GCV algorithm can effectively remove the noise from neurons discharging signal.The denoised waveform is smooth and continuous,and the signal peaks related details are retained.%为去除背根节神经元放电信号中的噪声，便于进一步分析信号，采用小波滤波法。先将含噪信号采用haar小波进行5层分解，然后在传统小波软阈值滤波的基础上，提出用GCV算法来确定最优阈值，最后进行信号重构。通过matlab仿真实验表明，采用了GCV算法的滤波方法能有效去除神经元放电信号中的噪声，去噪后信号光滑连续好，并且保留了信号峰值的相关细节。

摘要： 目的:观察DSA引导下背根节脉冲射频治疗带状疱疹后神经痛的疗效及其安全性.方法:60例带状疱疹后神经痛患者(VAS≥4.0),随机分为A、B两组,每组30例:A组使用加巴喷丁、阿米替林、奥施康定等药物治疗;B组在上述药物治疗的基础上.联合DSA引导下背根节脉冲射频进行治疗.观察患者射频治疗前,治疗后1、7、30、90、180 d的疼痛视觉模拟评分(VAS)、奥施康定使用量、药物副作用、治疗并发症等情况.结果:A组患者射频治疗前,治疗后1、7、30、90、180 d VAS分别为:(8.2±1.7)、(6.3±1.8)、(4.1±1.4)、(3.2 ±1.5)、(3.1 ±1.2)和(2.6 ±1.1)分.奥施康定使用量分别为:(28.8±5.5)、(35.6±8.5)、(42.3±8.9)、(18.6 ±4.3)、(10.7 ±2.4)和(8.1 ±1.6)mg.B组患者治疗前,治疗后1、7、30、90、180 d VAS分别为:(8.1 ±1.6)、(4.5 ±1.6)、(3.0 ±1.2)、(2.1±1.1)、(1.8±0.9)和(1.5±0.9)分.奥施康定使用量分别为:(29.1±5.8)、(17.6 ±5.4)、(12.4 ±3.8)、(5.1 ±1.6)、(6.0 ±1.3)和(4.2±0.9)mg,组间比较有显著差异(P<0.05),B组药物副作用减轻,无严重并发症.结论:DSA引导下背根节脉冲射频术能迅速减轻疼痛,降低镇痛药使用量并减少其相关副作用.是带状疱疹后神经痛的一种安全、有效的治疗方法.%Objective To observe the efficacy and safety of dosal root ganglion (DRG) pulsed radiofrequency for treating postherpetic neuralgia (PHN) guided by digital subtraction angiography (DSA). Methods 60 patients with PHN were randomly divided into two groups (each n= 30). Patients in Group A were treated with gabapentin, amitriptyline, and oxycodone, and patients in Group B were treated with DRG pulsed radiofrequency guided by DSA along with the same medicines used in Group A. VAS and oxycodone dosage before and 1d, 7 d, 30 d, 90 d, and 180 d after treatment, and side effects and complications during the treatment in the two groups were recorded. Results The VAS of patients in Group A and B before and Id, 7 d, 30 d, 90 d, 180 d after treatment were (8.2 ± 1.7) vs. (8.1 ± 1.6), (6.3 ± 1.8) vs. (4.5 ± 1.6), (4.1 ± 1.4) vs. (3.0 ± 1.2), (3.2 ± 1.5) vs. (2.1 ± 1.1), (3.1 ± 1.2) vs. (1.8 ±0.9), and (2.6 ±1.1) vs. (1.5 ±0.9), respectively (all P<0.05), and the oxycodone dosages were (28.8 ± 5.5) mg vs.(29.1 ± 5.8) mg, (35.6 ± 8.5) mg vs. (17.6 ± 5.4) mg, (42.3 ± 8.9) mg vs (12.4 ± 3.8) mg, (18.6 ± 4.3) mg vs. (5.1 ± 1.6) mg, (10.7 ± 2.4) mg vs. (6.0±1.3) mg, and (8.1 ± 1.6) mg vs. (4.2 ± 0.9) mg, respectively (all P < 0.05). The medicine side effects in Group B were obviously reduced, and no serious complications occurred. Conclusion DRG pulsed radiofrequency guided by DSA could rapidly relieve the pain of patients with PHN, reduce oxycodone dosage and its relative side effects, and may be a safe and effective method for treating PHN.

摘要： 目的:检测脊神经切断大鼠背根节(DRG)神经元重复放电能力和钠电流的变化,并研究介导其电流变化的钠通道亚型的表达情况.方法:脊神经切断术后2～8d慢性痛大鼠模型背根节急性分离,对中等直径DRG神经元运用全细胞膜片钳技术记录神经元放电和钠电流的变化.对背根节神经元进行RT-PCR检测,分析其钠通道亚型的表达情况.结果:电流钳下,实验组DRG神经元在电流刺激下产生重复放电,而对照组神经元多诱发单个动作电位,电压钳记录发现实验组背根节神经元快钠电流和持续性钠电流幅值均明显大于对照组,PCR结果显示,Nav1.3、Nav1.7和Nav1.8通道亚型mRNA表达显著增高.结论:钠通道介导了脊神经受损模型的DRG神经元兴奋性增高,持续性钠电流可能通过调节阁下膜电位振荡的产生调节神经元兴奋性.%Objective: To detect the changes of sodium influx in the transected spinal nerve rats and to investigate the expression of the sodium channel subunits.Methods: 2～8d after spinal nerve transection in rats model of chronic pain, the DRG were acutely isolated.The neuron discharge and sodium influx of medium-diameter DRG neurons were detected by the whole cell recording patch clamp technique.The DRG neurons, the expression of sodium channel subunits were detected by RT-PCR.Results: the repetitive firing of DRG neurons in experiment was induced under current stimulation, However, the single action potential was induced in control.According to the voltage-clamp recordings, the amplitude of the fast and persistent sodium current of DRG neurons in experiment wAS both obviously higher than that in control group.Meanwhile, the expression of sodium channel subunits Navl.3, Navl.7 and Navl.8 mRNA was obviously higher.Conclusions: The sodium channel increased the excitability of DRG neurons in the spinal nerve, and the persistent sodium current could adjust the excitability of neurons by regulating the subthreshold membrane potential oscillation.

摘要： 目的 观察重复腹腔注射右美托咪定(DEX)对神经病理性疼痛大鼠的痛觉过敏和背根神经节(DRG)中ERK信号通路激活的影响.方法 给坐骨神经部分结扎(PSNL)神经病理性疼痛大鼠重复腹腔注射不同剂量的DEX.观察各组大鼠的机械、热痛觉过敏阈值.行为学测试完成后用免疫荧光和Western blot方法检测大鼠手术侧L5 DRG中p-ERK的表达.结果 (1)腹腔注射DEX 40 μg/kg 7、14 d均明显减轻PSNL诱导的机械、热痛觉过敏(P＜0.05).而腹腔注射DEX20μg/kg对PSNL大鼠疼痛行为学无明显影响.(2)重复腹腔注射40μg/kg DEX 7、14 d p-ERK的平均荧光强度比同一时间点的PSNL组明显减弱(P＜0.05),但仍明显高于对照组(P＜0.05).重复腹腔注射20μg/kg对PSNL大鼠p-ERK的平均荧光强度无明显影响(P＞0.05).(3)Westem blot 结果显示重复腹腔注射40μg/kg DEX 7、14 d明显抑制PSNL诱导的p-ERK的蛋白表达增多(P＜0.05).重复腹腔注射20μg/kg对PSNL大鼠p-ERK的蛋白表达无明显影响(P＞0.05).结论 重复腹腔注射DEX能够减轻神经损伤引起的痛觉过敏,抑制外周初级感觉神经系统中ERK信号通路的激活可能是其缓解的疼痛症状的机制之一.%Objective To investigate the effect of systemic administration of dexmedetomidine, a selective alpha 2 adrenergic receptor agonist, on mechanical and thermal hyperalgesia and dorsal root ganglia ERK activation induced by neuropathic pain. Methods Intraperitoneal injection of dexmedetomidine was repeatedly given once daily for 7 days or 14 days with the first injection one day before partial sciatic nerve ligation ( PSNL) surgery. Mechanical and thermal nociceptive thresholds were assessed in all animals. Then, animals were killed at corresponding time points, and the L5 DRG was removed for L5 DRG ERK activation status analysis by using immunoflurecence and Western blotting. Results (1) Partial sciatic never ligation produced a robust mechanical and thermal hyperalgisia and ERK activation of the L5 DRG in P7 and P14 groups. At the dose of 40 μg/kg, dexmedetomidine significantly attenuated PSNL-induced ipsilateral hyperalgesia on the day 7 and 14 after surgery. But the dose of 20 μg/kg of dexmedetomidine had no effect on pain behaviorl; (2) PSNL-induced up-regulation of mean fluorescence intensity of pERK on ipsilateral side of the L5 DRG was significantly suppressed by day 7, 14 post-PSNL following 40 μg/kg dexmedetomidine application, whereas 20 μg/kg dexmedetomidine had no effect; (3) Western blotting revealed that up-regulation of the protein expression of p-ERK on ipsilateral side of the L5 DRG was significantly inhibited on the day 7, 14 post-PSNL following 40 μg/kg dexmedetomidine application,whereas 20 μg/kg dexmedetomidine had no effect. Conclusion Systemic dexmedetomidine inhibits the activation of ERK signals in L5 DRG, which is possibly associated with its antihyperalgesia in rats with neuropathtic pain.

摘要： 周围神经系统在损伤后能够再生，再生过程中许多基因和蛋白的表达发生改变-miRNA是一类进化上高度保守、能够在转录后调控基因表达的非编码RNA．本研究聚焦在大鼠坐骨神经缺损后，近端神经新miRNA的鉴定和功能注解．通过深度测序、计算机分析和Q．PCR验证，98个新miRNA在坐骨神经缺损后的0，1，4，7和14天被发现和鉴定．进一步预测了这些miRNA的靶基因，分析了靶基因参与的生物学过程，结果显示靶基因参与的生物学过程与坐骨神经缺损后神经损伤和再生的过程基本一致．本实验为miRNA在坐骨神经缺损后的调控作用的研究提供了基础，有助于周围神经损伤和再生分子机制的进一步研究．

摘要： 目的:观察芪麝丸对压迫损伤后不同时间点大鼠DRG 神经元SP 和CGRP 表达的影响. 方法:选择3 月龄SD 雄性大鼠96 只,SPF 级.大鼠随机分为3 个大组,即假手术组、模型组和芪麝丸组.每组根据不同时间点再分为3 天组、7 天组、14 天组、28 天组,每组8只.分别用Von Frey 纤维笔进行测试各组大鼠造模后50％撤足阈值;应用ELISA 法检测各组大鼠DRG 神经元SP 和CGRP 表达情况. 结果:模型组大鼠与假手术组相比大鼠50％撤足阈值均有显著性降低(P＜0.01);药物干预后,在芪麝丸7 日组、14 日组和28 日组大鼠50％撤足阈值均显著高于相应时间点各模型组(P＜0.01);其中,中药14 日组和中药28 日组50％撤足阈值与假手术组相比无显著性差异(P＞0.05).模型组各时间点SP 和CGRP 表达显著高于与假手术组(P0.05). 结论:芪麝丸可显著减轻大鼠神经根压迫损伤后痛觉过敏现象,其作用机制可能与抑制SP 和CGRP 神经递质的释放有关.

摘要： 本发明公开了一种原代培养背根神经节卫星胶质细胞的方法，所述方法操作如下：步骤(1)将新生小鼠或大鼠消毒后断头处死，解剖后取出脊柱，剔除脊柱上肌肉，剪开脊柱，在体视显微镜下清理血管和脊髓并取出DRG，剔除DRG上的神经纤维和被膜；步骤(2)将处理好的DRG采用DRG‑SGCs培养液培养，诱导大量的卫星胶质细胞从DRG中迁出，培养结束后加入胰蛋白酶消化，然后加入FBS终止消化，采用巴氏吹吸管轻轻吹打后，转移至离心管中进行离心；步骤(3)离心后弃上清液，然后加入培养液继续培养，得到卫星胶质细胞。本发明培养和纯化的卫星胶质细胞体外可存活较长时间，纯度高，稳定存活，可传代多代，形成细胞网络。

摘要： 本发明公开了一种靶向损伤节段背根神经节的pH响应镇痛药及其应用，属于生物医药技术领域。本发明基于在外周神经损伤引起的神经病理性疼痛条件下，受损伤节段背根神经节持续存在局部酸环境，提出以损伤节段背根神经节作为药物作用靶点来制备外周神经损伤镇痛药物，采用pH响应载体包埋镇痛药物，使其在受损伤节段的背根神经节部位释放并发挥作用，其镇痛效果达到同种药物全身作用的镇痛水平。本发明实现了在酸性病灶处精准用药，显著减少药物在其他部位的释放，大大降低现有阿片类用药存在的成瘾性等毒副作用。另外，药物在损伤节段背根神经节持续缓释，显著延长镇痛作用时间，避免镇痛药的过度使用。

摘要： 本发明提供了一种治疗带状疱疹和疱疹后神经痛的背根神经节分子手术刀，涉及手术刀技术领域，该装置包括第一端用于与主机相连接的电缆线段、与电缆线段的第二端相连接软管，软管为绝缘管，软管穿设在人体内，软管的第二端设置有多个软态环状的激发电极和回路电极，激发电极的数量与回路电极的数量相等，能够实现多点激发，提高了手术的效率，激发电极和回路电极沿软管的长度间隔设置，导管内设置有与激发电极相连接第一导线、与回路电极相连接的第二导线，第一导线、第二导线用于与电缆线段相连接，促使激发电极与回路电极之间产生高能电子、高能粒子、活性离子等离子体，直接作用与病变神经节、神经根、神经干和神经末梢，达到靶向治疗的目的。

摘要： 本发明属于组织样本获取技术领域，公开了一种适合于大鼠背根神经节DRG的取材方法，包括：取死亡的大鼠确定髂嵴位置，剪断分离脊柱；将分离出的脊柱放入双氧水中冲洗、浸泡一分钟；从取下的脊柱腰椎段横断面找到L5椎体；分别剪掉椎体两边此水平面的肌肉；依次分离汇合的脊神经、肌肉组织，取出背根神经节以及背根神经节两端的中枢端和外周端，只保留背根神经节；将背根神经节放入离心管中，再临时放入液氮中，再转入‑80°C冰箱保存。本发明能够快速定位脊神经节并且能保证背根神经节不被血渍污染，从而为组织学和形态学实验提供高质量的组织样本。本发明具有用时少，操作简便，取出来的背根神经节更完整，且不被血渍污染的优点。

摘要： 本发明公开了一种青春双歧杆菌及其培养方法和其促进背根神经节的神经元细胞生长中的应用，涉及微生物领域，本发明提供了一种青春双歧杆菌菌株MN‑84，可高产γ‑氨基丁酸，它们是神经系统中的神经抑制剂，对神经元的兴奋程度有着潜在的影响，在改善人抑郁等不良情绪上有着很大的调节作用；MN‑84还能通过促进背根神经节神经元细胞的生长，潜在参与人体神经系统的调节过程。

摘要： 本发明提供了一种背根神经节神经细胞与软骨细胞共培养的方法及应用，所述共培养方法包括：分别构建无需支架材料的背根神经节神经细胞球和无需支架材料的软骨细胞球，再将所得神经细胞球和软骨细胞球进行3D共培养；其中，构建所述背根神经节神经细胞球和3D共培养使用的培养基为神经细胞完全培养基，构建所述软骨细胞球使用的培养基为软骨细胞完全培养基。本发明还提供了一种背根神经节神经细胞球和软骨细胞球3D共培养体系及其神经浸润检测方法。所述共培养方法操作简单，细胞生理状态良好，可作为模型用于相关疾病致病机理的研究中，具有广阔的应用前景。

摘要： 本实用新型公开了一种用于神经痛背根神经节治疗的3D打印导航模板，包括导板本体和两到三个中空穿刺引导柱，所述导板覆盖患者胸背部穿刺侧区域，且贴合患者背部曲线，所述导板在每个穿刺位点对应的区域分别设有中空穿刺引导柱，所述导板本体上还设有用于嵌合在患者背部定位电极片金属突起上的多个固定孔。本实用新型使用时只需沿导板上穿刺引导柱向前推进穿刺针至预定深度即可，实现对DRG的精准定位穿刺、缩短手术时间、减少穿刺次数、减少患者创伤、降低手术风险，同时也减少了医患放射暴露时间。本实用新型导航模板只覆盖患者胸背部的穿刺区域，中间还设有孔洞，可以增加患者的舒适度，缩短打印时间。

摘要： 本实用新型涉及机械技术领域。腰突症动物模型背根神经节压迫用L型钢丝生产装置，包括剪切装置，还包括一支撑钢丝的支撑部；还包括钢丝弯折机构，钢丝弯折机构包括钢丝限位板，钢丝限位板与支撑部围成容纳钢丝的容纳通道；钢丝弯折机构还包括可竖直升降的升降成型块，升降成型块的顶部可拆卸连接有竖直设置的限位板，升降成型块上开设有用于局部嵌入钢丝的成型槽；钢丝限位板与升降成型块之间存有钢丝的端部向上弯折的间隙；还包括可左右滑动的滑台，滑台上安装有钢丝弯折机构以及剪切装置。本实用新型提供了一种钢丝L型弯折的成型装置。便于实现成型后的钢丝尺寸的一致性。

摘要： 本发明涉及一种从非神经细胞转化制备保留年龄特征的背根节神经元的方法。本发明的制备方法优化了关键的细胞直接转分化基因组合及调控表达水平的启动子，包装可以高效感染各种年龄供体源细胞的病毒，并采用合适的包被基质促进供体源细胞及转化后的神经细胞贴壁生长，并利用含有可以促进转分化的小分子化合物与生长因子的诱导分化培养液，在10‑14天内经过若干次更换分化培养液后转分化得到大量的背根节神经元，最后经过分离纯化获得高纯度的背根节神经元。

摘要： 本发明公开了小动物背根节长时程重复给药模型的建立及示教方法，给药模型的建立方法包括以下步骤：步骤一，暴露小鼠椎间孔；步骤二，确定给药管侧漏孔；步骤三，封闭进药口；给药模型的应用方法包括以下步骤：步骤一，测量基础机械痛阈；步骤二，建立给药模型；步骤三，核算背根节给药量；步骤四，注射CFA；步骤五，测量机械痛阈；步骤六，注入盐酸吗啡溶液；步骤七，再次测量机械痛阈；步骤八，结果分析；本发明具备以下的优点：(1)给药位置和给药剂量精确，可重复性强，成功率高；(2)可长时程反复给药；(3)固定牢靠，给药管不易脱出；(4)可操作性强，器材价格低廉，操作简单，易于推广。