摘要：
Objective To investigate the effects of high-fat diet on pancreatic acinar cells' IP3 expression and CCK-induced amylase release in rats.Methods Male Wistar rats were divided into high-fat diet group and normal diet group,they were fed for 4 weeks.Blood triglycerides,cholesterol,amylase and glucose levels were determined by automatic biochemical analyzer.Pancreatic tissues were taken for histopathological observations.Pancreatic acinar cells were isolated and cultured,and intracellular inositol 1,4,5-trisphosphate (IP3) was detected using a commercial kit.Amylase release rates were measured after CCK-8 stimulation.Results The rats in high-fat diet group appeared hyperlipidemia,vacuolization of acinar cells and the lymphocytes appeared around the acinar cells can be seen on the pancreatic tissue pathology staining.The levels of IP3 in acinar cells of rats fed a high-fat diet were higher than that of normal rats [(31.807 ± 3.448) pmol/106 cells vs (24.632 ± 3.649) pmol/106 cells,t=7.479,P＜0.001];and amylase release rate in these rats'acinar cells were also higher than those of normal rats [when CCK-8=0.01 nmol/L:( 11.056 ±3.369)％ vs (7.354 ± 2.181) ％,t=3.912,P＜0.001;when CCK-8=1 nmol/L:( 13.854 ± 4.087 ) ％ vs (9.432 ±2.477) ％,t=3.939,P＜0.001 ) after CCK-8 stimulation in different concentrations.Additionally,there was a positive co-relationship between acinar cell's IP3 level and amylase release (r=0.896,P＜0.001 ).Conclusion Chronic high-fat diet induces hypersensitivity for pancreatic acinar cells' exocrine function,and IP3 as a signal molecule may play an important role in this process.%目的 探讨高脂饮食对胰腺腺泡细胞内三磷酸肌醇( IP3)表达及淀粉酶释放的影响.方法 雄性Wistar大鼠分为高脂饮食组和正常饮食组,分别喂养4周,全自动生化仪检测血液甘油三酯、胆固醇、淀粉酶和葡萄糖浓度,并观察胰腺病理组织学变化.两组大鼠分离并培养胰腺腺泡细胞,应用[3H]-IP3检测试剂盒检测细胞内IP3浓度；并应用胆囊收缩素(CCK)-8对腺泡细胞刺激培养,检测两组大鼠腺泡细胞淀粉酶释放率.结果 高脂饮食大鼠出现高脂血症,其胰腺组织病理染色发现腺泡细胞空泡化,腺泡细胞周围淋巴细胞浸润.高脂饮食大鼠的腺泡细胞IP3含量明显高于正常大鼠[(31.807±3.448) pmol/106 cells与(24.632±3.649) pmol/106 cells,t=7.479,P＜0.001]；且高脂饮食大鼠较正常组大鼠的腺泡细胞在不同浓度CCK-8(0.01 nmol/L和1 nmol/L)刺激下的淀粉酶释放率均明显增高[(11.056±3.369)％与(7.354±2.181)％;t=3.912,P＜0.001;(13.854±4.087)％与(9.432±2.477)％,t=3.939,P＜0.001).高脂饮食组大鼠的腺泡细胞IP3含量和CCK-8(1 nmol/L)刺激下的淀粉酶释放率呈正相关性(r=0.896,P＜0.001).结论 长期高脂血症引起大鼠腺泡细胞对CCK刺激的外分泌敏感性增加,IP3作为信号分子具有重要作用.
