摘要：
目的 介绍对清醒小鼠脑部进行急性在体细胞外电生理记录的方法及一些基本的数据处理方法. 方法 采用多通道硅电极及清醒小鼠头部固定系统在小鼠清醒状态下对其脑部神经元做急性细胞外记录.首先,在小鼠的颅骨表面粘贴一个不锈钢头板,术后小鼠恢复一周后,将不锈钢头板与清醒小鼠头部固定系统相连使小鼠的头部得以固定,其四肢踩在一个漂浮的泡沫球上可以自由的活动,对小鼠进行连续3 d的适应性训练,每天的训练时间递增,使之能够逐渐熟悉并适应电生理记录的环境,然后,将多通道急性硅电极插入小鼠脑内,应用多通道电生理记录系统,记录神经元的动作电位(spike)及局部场电位(local field potential,LFP),并应用Offline Sorter软件对单个神经元(Single Unit)进行划分以及应用NeuroExplore软件对LFP通过滤波的方法划分出4种震荡成分Delta、Theta、Beta和Gamma震荡. 结果 在清醒小鼠脑内成功记录到了神经元的动作电位及LFP,并通过软件划分出了Single Unit和Delta、Theta、Beta和Gamma 4种震荡成分.结论 应用清醒小鼠神经元急性细胞外记录的方法,能够很好的记录到动作电位及LFP.%Objective Introduction to the method of acute extracellular recording of the brain of awake mice in vivo and some basic data analysis methods.Methods We applied multi-channel silicon electrode and awake mouse head-fixed system to perform acute electrophysiological recording in the awake mice.First,a head-plate adhered to the skull of the mouse,after surgery,one week's rest was given,the mouse was then mounted to the head-fixed system by screwed together the head-plate of the mouse and the head-plate holder of the head-fixed system.The limbs of the mouse could freely move in a floating ball of the head-fixed system.Three consecutive days adaptive training were performed in order to habituate the mice of the recording background,then performed the acute electrophysiological recording by use of multi-channel electrode and multi-channel electrophysiological recording system,to record the spikes and local field potentials (LFP) in neurons of awake mice,and applied Offline Sorter software to isolate the Single Unit as well as NeuroExplore software to separated the Delta,Theta,Beta and Gamma oscillations from the LFP by means of filtering.Results We successfully recorded the spikes and LFPs in neurons of awake mice,and isolated the single units as well as separated the Delta,Theta,Beta and Gamma oscillations from LFP by the analysis software.Conclusion Application of acute extracellular electrophysiological recording methods can successfully record the spikes and LFPs in neurons of awake mice.
