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离子束注入

离子束注入的相关文献在1988年到2022年内共计138篇,主要集中在农作物、化学工业、农学(农艺学) 等领域,其中期刊论文76篇、会议论文9篇、专利文献274281篇;相关期刊61种,包括泉州师范学院学报、郑州大学学报(理学版)、长江大学学报(自科版)农学卷等; 相关会议9种,包括第十三次全国环境微生物学术研讨会、第二届全国核技术及应用研究学术研讨会、2007年工业生物技术研发及生产生物质能和生物基化学品技术交流与发展研讨会等;离子束注入的相关文献由339位作者贡献,包括余增亮、张祥胜、J·G·布莱克等。

离子束注入—发文量

期刊论文>

论文:76 占比:0.03%

会议论文>

论文:9 占比:0.00%

专利文献>

论文:274281 占比:99.97%

总计:274366篇

离子束注入—发文趋势图

离子束注入

-研究学者

  • 余增亮
  • 张祥胜
  • J·G·布莱克
  • 刘桂君
  • 周思静
  • 孟佑婷
  • 尚宏忠
  • 杨素玲
  • 顾海科
  • 唐纳德·W·贝里亚
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 刘国生; 李金鑫; 刘磊; 谷艳昌; 许航
    • 摘要: 为了获得腺苷高产菌株,以肌苷产生菌枯草芽孢杆菌(Bacillus subtilis)HSD1206(Ade-、Thi-、SGr)为出发菌株,通过硫酸二乙酯(DES)处理、N离子束注入进行单因子和复合诱变.参照菌种致死率曲线,确定N离子束注入250 s与DES处理10 min为最佳诱变时间.经过多轮诱变和筛选,获得了黄嘌呤、硫胺素、组氨酸三重缺陷型,腺嘌呤脱氨酶活性缺失及磺胺胍抗性菌株DI4-24和DI4-182,其遗传标记为Xan-、Thi-、His-、Deam-、SGr,连续传代后性状稳定,在优化的发酵培养基中发酵后腺苷产量可分别达到18.56 g/L和17.13 g/L.由此证明,通过物理、化学复合诱变,阻断枯草芽孢杆菌营养缺陷型菌株产肌苷途径,打通合成腺苷途径的诱变思路在实验室环境下是可行的.%The inosine producing bacterium Bacillus subtilis HSD1206 (Ade-Thi-SGr) was selected as parent strain and treated with diethyl sulfate (DES) and N ion beam injection for single factor or compound mutagenesis to select adenosine producing strain after several rounds of mutation and breeding.Results showed that the best mutagenesis time was 250 s by N ion beam injection or 10 min by DES treatment according to the strain lethality curve.The strains which showed xanthine,thiamine,histidine triple defect type,lacked adenine deaminase activity and resisted sulfaguanidine were named DI4-24 and DI4-182,and the genetic markers were Xan-Thi-His-Deam-and SGr.They could steadily accumulated adenosine in the optimization fermentation medium to 18.56 g/L and 17.13 g/L respectively after consecutive culture.In conclusion,the thought of blocking the way to product inosine and inducing the way to synthesize adenosine of Bacillus subtilis auxotroph strain by physical and chemical mutagenesis is feasible in the laboratory environment.
    • 唐楠; 任翱博; 刘才; 李卫; 张静全; 武莉莉
    • 摘要: ZnTe:O高失配合金半导体是实现中间带太阳电池的有效途径,其制备通过如下几步实现:1)分子数外延(MBE)设备进行ZnTe单晶生长;2)将分子束外延制备的单晶ZnTe进行离子束注入;3)通过快速激光退火处理来实现ZnTe晶体质量的提升,修复晶格损伤.MBE所获得的最优晶体质量的条件是Zn源温控制在260°C,Te源温控制在290°C.掺杂O为1.25×1021cm-3时,所获得的中间带效果最好,并且在经过激光快速退火处理后,中间带的强度显著提升.%ZnTe:O highly mismatched alloys is an effective way to achieve intermediate band solar cell.The fabrication of ZnTe:O is as follows:(a) the growth of ZnTe single crystal by molecular beam epitaxy;(b) the implantation of O ions to ZnTe;? the rapid annealing by pulsed laser to improve the quality of ZnTe single crystal and repair the crystal damage.The best condition to get ZnTe with high quality is to fix the temperature of Zn and Te at 260 °C and 290 °C,respectively.When the O concentration is 1.25 × 1021 cm-3,the intermediate band reaches its peak and its intensity is obviously enhanced after the pulsed laser annealing.
    • 郝杰; 阮少宁; 林思祖; 张涛
    • 摘要: 在能量50 keV的条件下,将不同剂量的Fe +及Ti +注入杉木种子,观察离子束注入后对种子萌发及幼苗形态学的影响。结果表明:Fe +及Ti +注入对种子发芽有延迟效应,且发芽率随离子束注入剂量的增高呈下降趋势;离子束注入剂量对杉木主根初期的生长具有显著抑制作用,不同剂量Fe +的注入可使根长大幅度下降,即根长对Fe +的敏感程度更高;处理组与对照相比苗高均值相差不大,但个体上经Fe +处理的突变率高于Ti +处理,经离子注入处理组共有7株优势苗入选。%The effect of different dosage injection of Fe +and Ti +into Cunninghamia lanceolata seeds under 50 keV energy condition on seed germination and morphological characteristics and growth indexes of the seedlings were observed in the study.The results show that Fe +and Ti +injection had a delay effect on seed germination,the germination rate dropped along with the dosage increase of Fe +and Ti +ions injected into the seeds.The early stage of seedling root growth was significantly inhibited by ion beam injection.The root length of the seedlings was sharply reduced along with the increment of Fe +dosage injected,indicating that the root length of C.lanceolata seedlings was highly sensitive to Fe +ion.There were no significant differences in seedling height among the treat-ments and the control group,but the mutation rate of seedligns injected with Fe +ion was higher than that of injec-ted with Ti +ion.7 superior seedlings were selected from treatment group with ion beam injection.
    • 宫国安; 钱朗; 邹畅
    • 摘要: 以两个玉米自交系联87和丹黄25为材料,采用能量为8.0×1015焦耳的 N +,在5.0×1020个/ cm2的相同剂量下,分别进行离子束注入处理。设置种胚面(正面)、非种胚面(侧面)胚面注入加激活、非种胚面加激活等处理。在相同种植条件下对经离子束处理的 M1代的生物学效应和 M2的变异频率进行研究。结果表明:在不同处理下两个自交系的出苗率均较 CK 均下降,其差异达到极显著水平;联87在不同处理下的平均变异频率为9.3%,其中侧面注入加激活变异频率最大,为23.1%;丹黄25在不同处理下的平均变异频率为3.5%,其中正面注入加激活变异频率最大,为5.6%。从变异谱看,两个自交系均出现在熟期、育性、抗性、品质、双穗率、株高和穗长等变异,特别是出现3个以上性状同时变异而且是正向的期待性状,运用模拟法测定变异株系配合力,联87正向变异不明显,丹黄25获得一个株高变矮、叶片变宽、品质变好、双穗率达83%优良穗行。
    • 谢飞; 崔国英; 王翀; 江兵; 沈联兵; 虞龙
    • 摘要: U sing C orynebacterium glutam icum as originalstrain,through low energy N + im planting into C .glutam icum ,com bining w ith isoleucine hydroxam ic (IleH x)and otheram ino acid structure analogues to conductdirected screening,one high L-isoleucine yield m utantstrain w as obtained. A fter72 h ferm entation in shaking flask,the concentration ofL-Ile w as 21-22 g/L,and after51 h ferm entation in 50-liters ferm entor,the concentra-tion ofL-Ile w as32 g/L.%以谷氨酸棒状杆菌(C orynebacterium glutam icum)为出发菌株,利用低能氮离子束注入对其进行多次诱变,结合异亮氨酸氧肟酸盐(IleH x)等氨基酸结构类似物定向筛选,得到一株稳定的高产L-异亮氨酸菌株:摇瓶培养72 h,产酸可达21~22 g/L,在50 L发酵罐上发酵51 h,产酸可达32 g/L。
    • 郝杰; 阮少宁; 林思祖; 张涛; 薛明瑜
    • 摘要: 研究比较不同离子注入对杉木生长差异,为离子注入技术在杉木育种上的研究提供参考依据.以能量50 keV,不同剂量 Fe+及 Ti+注入杉木种子,观察注入后其对杉木幼苗苗高及相对电导率影响.Fe+及Ti+注入对苗高长生均呈波动上升趋势,Fe+ D4剂量对苗高促进作用较强,Ti+ D3剂量对苗高促进作用较好,随着幼苗的生长,苗高增幅之差表现出下降的趋势.Fe+及 Ti+注入处理组的电导率均表现出先上升后下降的趋势,电导率在 D3剂量时都上升达到最高点,分别上升了9.5%和4.8%;同剂量下 Fe+的上升幅度均大于Ti+,对电导率的影响更显著.Fe+及 Ti+对幼苗的苗高具有促进作用,并随注入剂量升高促进作用有越强的趋势;Ti+注入相比 Fe+注入,对细胞膜造成损伤程度低。%This paper studies the growth that varied significantly of Chinese fir comparison different ion implantation for ion implantation studies on Chinese fir breeding technology to provide reference. Under energy of 50 keV,Fe+ and Ti+ ,different dose injection to the fir seeds observe fir seedling height and relative conductivity index changes.Fe+ and Ti+ injection have the trend of rising on seedling height,Fe+ D4 doses of injected on the seedling height promote even more sharply,Ti+ D3 doses of injected have better effect to promote the seedling height,and to promote the implantation dose increases with the stronger tendency,However,no obvious significant .with the growth of seedlings,seedling height increment showed a downward trend.Relative conductivity all offered upgrade firstly than descending latter tendency.The conductivity is increased to the highest dose D3 point,increasing 9.5% and 4.8%.Rise of Fe+ ions with a dose greater than Ti+ ions,more significant to the influence of the conductivity.Fe+ and Ti+ injection have certain stimulating function for its height,Ti ion implantation compared to the Fe ions,resulting in low levels of cell membrane damage.
    • 郝杰; 阮少宁; 林思祖; 张涛
    • 摘要: 在能量50 keV的条件下,将不同剂量的Fe+及Ti+注入杉木种子,观察离子束注入后对种子萌发及幼苗形态学的影响。结果表明:Fe+及Ti+注入对种子发芽有延迟效应,且发芽率随离子束注入剂量的增高呈下降趋势;离子束注入剂量对杉木主根初期的生长具有显著抑制作用,不同剂量Fe+的注入可使根长大幅度下降,即根长对Fe+的敏感程度更高;处理组与对照相比苗高均值相差不大,但个体上经Fe+处理的突变率高于Ti+处理,经离子注入处理组共有7株优势苗入选。
    • 于平; 孙弘宇
    • 摘要: In this article,azelaic acid-production strains were mutated and screened by means of ion implantation.A mutation strain was obtained with azelaic acid yield of 35.81 g · L-1,which was 74.17% higher than that of the strating strain.%采用离子束注入的方法对产壬二酸菌种进行诱变筛选,获得一株壬二酸产率达到35.81 g·L-1的菌株,壬二酸产率较出发菌株提高了74.17%.
    • 谢瑀婷; 李颖颖; 郑惠; 奚涛
    • 摘要: A mutant strain was screened from G. Lucidum. Meiguo Lingzhi by the technique of ion implantation. Under the implantation conditions of 20 KeV, 2×1016ions/cm2, a high yield exopolysaccha-ride strain has been selected from mutant strains. As a result of that the exopolysaccharide yield was 2. 71 g/L, and increased by 15. 32% than that of the comparison strain. The optimal medium was: glucose 45 g/L, corn meal 40 g/L, soybean meal 10 g/L, yeast extract 0. 5 g/L, KH2PO4 0. 5 g/L, MgSO4 ? 7H2O 0. 1 g/L and Vb, 25 jug/L. After 120 hours fermentation by the batch process , the exopolysaccharide yield could reach 3. 38 g/L.%以美国大灵芝为出发菌株,利用N+离子注入技术选育出一株遗传性状稳定的灵芝多糖高产菌株.结果表明:在20 KeV、2×1016ions/cm2的注入条件下灵芝菌株G13的胞外多糖的产量为2.71 g/L,比对照菌株提高了15.32%.采用单因素法和正交实验法得到的培养基组分质量浓度优化组合为:葡萄糖45g/L,玉米粉40g/L,黄豆粉10g/L,酵母膏0.5g/L,VBl 25 μg/L,KH2PO4 0.5 g/L,MgSO4·7H2O 0.1g/L.进行分批发酵实验,发酵120 h灵芝胞外多糖的产量达到3.38g/L.
    • 代西梅; 张佳佳; 杨旭; 黄群策
    • 摘要: 以水稻品种紫粳二倍体和四倍体为实验材料,以低能氮离子束为诱变源,研究了离子束诱变对水稻根尖细胞染色体的影响.结果发现,低能离子束注入引起了水稻根尖分生区细胞有丝分裂异常,出现许多染色体畸变类型,包括后期染色体桥、落后染色体、染色体断片、中期染色体单价体等.染色体畸变率并不是随着剂量的增加呈现直线上升的趋势,而是有一定的摆动性,但总体趋势是注入剂量越大,畸变率越高.由此可见,离子注入引起水稻染色体异常是其发生遗传性变异的细胞学基础.%The seeds of diploid and tetraploid rice were implanted by nitrogen ion beam. The cytological effects of the root tip cells were studied. The results showed that ion beam implantation could inhibit the survival rate and lots of types of chromosome aberration were observed in the mitosis of root tip cells of MO generation, such as chromosome bridge, chromosome fragment, univalent and polyvalent. Chromosome aberration rate was not proportional to N + ion treatment dosages. But as a whole, the more of the dose, the higher of the chromosome aberration rate. The results proved that ion beam could cause abnormity of chromosomes, which might be the cytological reason that leading to the heritable mutations.
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