摘要：
[Objective]Screening the measures to promote the survival rate of grafting and the growth of the new shoots during the grape hard branch grafting.[Method]The annual branches of '5BB' and 'Cabernet Sauvignon' were taken as experimental materials.Removed the buds from the scion, immersed the scion in CIPC, as well as immersed the rootstock and grafted union point in ABT rooting powder No.2 as experimental treatments, respectively.Immersed with clear water was as the control of CK.The branches were grafted with"Ω"grafting machine made in Germany.Scion sprouting rate, callus formation rate of grafted union point, rooting rate of rootstock, survival rate and new shoot growth were investigated in order to explore the measures to promote the survival rate and the new shoot growth of grafted grape propagules.[Result]The callus formation of grafted union point was promoted, the survival rate of graftedgrape propagules was increased, and the growth of new shoots was not obvious after removed the buds from the scion.After immersed the scion in CIPC, the callus formation rate of grafted union point was decreased, thus, the affected the survival rate of grafted grape propagules, and the new shoot growth was significantly inhibited.After immersed the rootstock and grafted union point in ABT rooting powder No.2, the callus formation rate of the grafted union point was increased, the low concentration of ABT promoted the survival of grafted grape propagules, and the survival rate was the highest at 250mg/L, which was up to 80％, and the new shoot growth reached the maximum.However, the survival rate at the high concentration of ABT was decreased, and the new shoot growth was obviously inhibited.[Conclusion]Scion sprouting, low concentration of ABT rooting powder No.2 treatments of grafted union point and rootstock can promote the survival rate of grafted grape propagules.The survival rate of grafted grape propagules reaches to 80％ after 250mg/L of ABT treatment, and the growth is also obviously promoted.%[目的]筛选葡萄硬枝嫁接过程中促进嫁接成活率及新梢生长的措施.[方法]以'5BB'和'赤霞珠'一年生枝条为试材, 采用接穗抹芽、CIPC处理、砧木及嫁接口ABT生根粉2号处理, 清水为对照, 通过德国产"Ω"嫁接机嫁接.对接穗萌芽率、嫁接口愈伤组织形成率、砧木生根率、嫁接成活率及新梢生长量进行测定分析, 以探讨其对嫁接成活率及新梢生长的影响.[结果]接穗抹芽, 促进嫁接口愈伤组织形成, 嫁接成活率升高, 新梢生长变化不明显;接穗CIPC处理, 嫁接口愈伤组织形成率降低, 从而影响嫁接苗成活, 新梢生长受到明显抑制;砧木及嫁接口ABT处理, 嫁接口愈伤组织形成率升高, 低浓度ABT促进嫁接苗成活, 250mg/L成活率最高, 达80％, 新梢生长量最大;高浓度ABT处理成活率降低, 新梢生长受到抑制.[结论]葡萄硬枝嫁接过程中接穗抹芽、嫁接口及砧木低浓度ABT 2号处理均能提高嫁接苗成活率;250mg/L ABT处理, 嫁接苗成活率达80％, 同时对嫁接苗生长促进作用明显.
