生长物质

摘要： [目的]探讨老年高血压合并慢性心力衰竭(CHF)患者血清转化生长因子-β1(TGF-β1)、结缔组织生长因子(CTGF)水平变化及其与心功能的相关性.[方法]选取2018年1月至2018年7月本院收治的130例老年高血压合并CHF患者的临床资料(观察组),同时选择于本院就诊的老年单纯高血压患者60例作为对照组.比较两组血清TGF-β1、CTGF、心功能相关指标,并采用Pearson相关分析血清TGF-β1、CTGF与心功能的相关性.[结果]观察组左室射血分数(LVEF)显著低于对照组,左室舒张末期内径(LVEDd)、左室质量指数(LVMI)显著高于对照组,差异有统计学意义(P＜0.05).观察组血清TGF-β1、CTGF水平高于对照组,差异具有统计学意义(P＜0.05).观察组NYHA分级Ⅱ级、Ⅲ级、Ⅳ级患者血清TGF-β1、CTGF水平随着NYHA分级升高逐渐升高,组间比较差异具有统计学意义(P＜0.05).高血压合并CHD患者血清TGF-β1、CTGF水平水平与LVEF呈显著负相关性(P＜0.05);血清TGF-β1、CTGF水平与LVM[呈显著正相关关系(P＜0.05).[结论]老年高血压合并CHF患者血清TGF-β1、CTGF处于较高水平,且与患者心功能具有密切相关性.

摘要： Platelet-rich plasma ( PRP) has been used to improve bone and soft tissue repair for decades , including cutaneous wounds, bone defect, osteoarthritis, and tendinopathy.However, the effectiveness of PRP in treating the tissue with poor blood supply such as tendon and cartilage is still controversial currently .In this paper , some common problems and controversies about the clinical applications of PRP are discussed .%富血小板血浆（ PRP）已在临床上应用多年，现已被广泛用来修复骨与软组织损伤，包括创面，骨缺损，骨关节炎，肌腱病等等。 PRP在贫血供组织如肌腱、软骨的修复方面，目前存在争议较多。本文就PRP在临床应用中出现的一些问题和争议进行讨论。

摘要： 富血小板血浆（ PRP）已被广泛用在临床上修复骨与软组织损伤。 PRP促进组织修复的机制在于血小板激活后释放多种生长因子，加速了修复细胞的增殖分化和细胞外基质的合成。近几年，随着胞外囊泡（胞外体和外泌体）研究的增多和深入，发现胞外囊泡在组织修复中也发挥了重要作用。 PRP中含有大量的胞外囊泡，但目前关于PRP来源的胞外囊泡研究还非常少。本文对PRP胞外囊泡的生物学特点和其促进组织修复的可能机制作一综述。%Platelet-rich plasma ( PRP ) has been widely used for the treatment of bone and soft tissue injury.The mechanism of PRP improving tissue regeneration is associated with multiple growth factors released after platelet activation , which accelerate cellular proliferation and differentiation and extracellular matrix synthesis .In recent years , studies about extracellular vesicles ( including ectosomes and exosomes ) have developed greatly , and found that extracellular vesicles play important roles in tissue healing.PRP contains large numbers of microvesicles , however, there are just few studies on PRP-derived extracellular vesicles now .The biological characteristics of PRP-derived extracellular vesicles and the possible mechanisms of promoting tissue repair were reviewed in this article .

摘要： Objective To investigate the influence of the serum vascular endothelial growth factor (VEGF) in the glioma patients by the malignant degress.Methods Quantitative analysis the serum VEGF levels with the double antibody sandwich assay (ELISA) for 35 cases of glioma patients and 10 cases of healthy human and analyze the correlation of them.Results Mean serum VEGF expression in glioma patients was 120.71 ±45.99 pg/ml,normal value of the healthy people was 63.70 ± 6.50,the difference was statistically significant (P ＜0.05); serum VEGF expression was 156.43 ± 14.90 pg/ml in high-grade glioma patients (Ⅲ-Ⅳ grade) and 67.14 ± 6.12 pg/ml in low-grade glioma patients (Ⅰ-Ⅱ grade),the difference was statistically significant (P ＜ 0.05) ; there' s no different from low-grade glioma patients (Ⅰ-Ⅱ grade) and nomal(P ＞ 0.05).Conclusion The serum VEGF levels in the glioma patients was influence by the malignant degress.%目的 探讨胶质瘤肿瘤恶性度对患者血管内皮生长因子(VEGF)在血清中的表达水平的影响.方法 运用酶联免疫吸附双抗体夹心法(ELISA)定量分析对35例胶质瘤患者及10例健康人血清VEGF水平进行检测,并进行统计学分析.结果脑胶质瘤患者血清VEGF表达均值为120.71±45.99 pg/ml,正常健康者均值为63.70±6.50,差异有统计学意义(P＜0.05);高级别胶质瘤(Ⅲ-Ⅳ级)患者血清VEGF为156.43±14.90 pg/ml,低级别胶质瘤(Ⅰ-Ⅱ级)患者为67.14±6.12 pg/ml,差异有统计学意义(P＜0.05);健康者与低级别胶质瘤(Ⅰ-Ⅱ级)患者血清VEGF水平差异无统计学意义(P＞0.05).结论 胶质瘤患者血清中VEGF水平明显受到肿瘤恶性度的影响.

摘要： 目的探讨 Wnt2 b和β-catenin在胃癌中的表达及其临床意义。方法采用免疫组织化学染色检测48例胃癌组织和48例癌旁正常组织中 Wnt2b和β-catenin的表达,并分析其临床意义。结果 Wnt2b 和β-cate-nin在胃癌组织中的表达率分别为81.3%、66.7%；显著高于癌旁正常组织(12.5%和0%),差异具有统计学意义(χ2=45.553,P<0.05)；Wnt2b的表达与TNM分期、分化程度、淋巴结转移相关(P <0.05)；β-catenin的表达与TNM分期、分化程度、组织学类型、淋巴结转移和癌侵犯神经相关(P <0.05)；且 Wnt2 b 与β-catenin蛋白的表达呈显著正相关性(r=0.340,P<0.05)。结论 Wnt2b和β-catenin在胃癌的进展、分化、浸润和转移中起重要作用,是判断胃癌生物学行为的良好指标。%Objective]To explore the expression and clinical significance of Wnt2b andβ-catenin in gastric carcino-ma.[Methods]The expressions of Wnt2b andβ-catenin in 48 gastric cancer tissues and 48 normal paracancerous tissues were detected by immunohistochemistry method.Clinical significance was analyzed.[Results]The expressions of Wnt2b andβ-catenin in gastric carcinoma tissues were 6 6 .7% and 3 5 .4% respectively,which were markedly higher than those in normal paracancerous tissues,and there were significant differences(χ2=45.553,P<0.05).The expression of Wnt2b was correlated with TNM stage,differentiation degree and lymph node metastasis(P<0.05).The positive expression ofβ-catenin in cancer tissues was obviously higher than that in normal paracancerous tissues(χ2=48.000,P<0.05),and correlated with TNM stage,differential degree,lymph node metastasis and nerve invasion(P<0.05).The protein ex-pression of Wnt2b was positively correlated with the protein expression ofβ-catenin(r=0.340,P<0.05).[Conclusion]Wnt2b andβ-catenin play important roles in the progression,differentiation,infiltration and lymph node metastasis of gastric carcinoma,and may be the good indicators for j udging the biological behavior of gastric carcinoma.

摘要： 富含半胱氨酸的酸性分泌蛋白（SPARC）在大多数组织中表达，它是一种结合钙、胶原和羟基磷灰石的多功能糖蛋白及细胞外调节大分子。循环血液中的 SPARC 主要来源于脂肪细胞，它参与胚胎发育、血管生成、细胞迁移和增殖、纤维化、组织重塑及创伤修复、肥胖、胰岛素抵抗等多种过程，为今后临床研究进展及治疗如肥胖、糖尿病及其并发症、心肌梗死等提供了新方向。

摘要： Objective To observe the effect of BMP2 on bone marrow mesenchymal stem cells (BMSCs) differentia-ting into hepatocytes .Methods BMSCs in thighbone of rats were isolated and cultured using adherent culture method . The third generation of amplification of BMSCs in vitro was made into cell climbing piece , and directional inducting hepato-cyte.BMSCs was divided into Hepatocyte growth factor (HGF) group , HGF +BMP2 group, BMP2 group and blank con-trol group depending added inducing factor .Cells were collected after culturing about 10 days.Changes in cell morphology was observed .Using enzyme-linked immunosorbent assay to culture supernatant of liver cell-specific marker alpha-fetopro-tein (AFP), albumin (ALB), Cytokeratin immunohistochemistry assay cells -18 (CK-18).Results ALB, AFP and CK-18 in HGF group and HGF +BMP2 group can be detected , and were higher than those in HGF +BMP2 group ( all P<0.05).Conclusion BMP2 can not alonely induce BMSCs differentiating into hepatocytes ,enhance the effect of HGF-in-duced hepatocyte differenting into BMSCs .%目的：观察骨形态发生蛋白2（BMP2）在骨髓间充质干细胞（BMSCs）向肝细胞分化中的作用。方法采用贴壁法分离培养大鼠股骨BMSCs ，将体外扩增的第3代BMSCs制作细胞爬片，并行肝细胞定向诱导。根据诱导因子不同分为：肝细胞生长因子（ HGF）组、HGF＋BMP2组、BMP2组及空白对照组。培养10 d左右收集细胞，观察各组细胞形态的变化，并采用ELISA法检测培养液上清中肝细胞特异性标志物甲胎蛋白（ AFP）、白蛋白（ ALB），免疫细胞化学法检测诱导分化后细胞CK-18的表达。结果 HGF组和HGF＋BMP2组可检测到ALB、AFP及CK-18，且HGF＋BMP2组ALB、AFP及CK-18明显高于HGF组（P均＜0．05）。结论 BMP2不能单独诱导BMSCs向肝细胞分化，但能增强HGF诱导BMSCs向肝细胞分化的作用。

摘要： 为了更好地提高烟草催芽种子的质量，尤其是改善陈种子的萌发能力，实验采用不同浓度的MS营养液、GA3、IBA、6-BA及4者复配对K326、NC89新陈2类种子进行催芽处理，统计分析不同条件催芽处理后的种子萌发率、各项生理生化指标变化以及萌发相关基因的表达。结果表明，各因素单独催芽处理，在适宜浓度范围内均能促进种子萌发。而采用生长物质复配配方（1/20 MS+100 mg/L GA3+2 mg/L IBA+0.2 mg/L 6-BA和1/20 MS+100 mg/L GA3+2 mg/L IBA+2 mg/L 6-BA）催芽处理，种子萌发迅速且各项生理生化指标显著优于对照。此外，WRKY2基因的表达量在催芽处理后显著提高。%To improve the quality of primed tobacco seeds, especially the germination ability of old seeds, studies were conducted on the seeds of K326 and NC89 of different years. The seeds were primed by different concentration of MS medium, GA3, IBA and 6-BA, and then the germination rate, physiological and biochemical indices and the expression of germination related gene were detected. The results showed that after priming treatment by proper concentration of each factor, the germination rate of flue-cured tobacco seeds were promoted, and the combination of different growth regulators (1/20 MS+100 mg/L GA3+2 mg/L IBA+0.2 mg/L 6-BA and 1/20 MS+100 mg/L GA3+2 mg/L IBA+2 mg/L 6-BA) had the best effects, After treatment of which the seeds geminated rapidly and each physiological and biochemical index was better than control significantly. What’s more, the expression level of WRKY2 was enhanced remarkably after priming treatment.

摘要： Objective :To explore the expression of VEGF and PCNA in lung cancer and the relationship with prognosis .Methods :Immunohistochemistry was used to detect VEGF and PCNA in 62 samples with lung cancer and 50 samples with normal lung tissue .The difference of VEGF and PCNA and their relationship with the prognosis of lung cancer were studied .Results :The positive rates of VEGF and PCNA were 67 .7% and 72 .6% ,of which were much higher than those in normal lung tissue (14 .0% and 12 .0% ,P<0 .05);The expression of VEGF closely related with differentiation ,lymphatic metastasis ,tumor size and clinical stage (P<0 .05);The expression of PCNA correlated with differentiation ,lymphatic metastasis and clinical stage (P<0 .05);The 5 year survival rates were much lower on the VEGF and PCNA positive patients (P<0 .05) .Conclusion :VEGF and PCNA closely relate with the occurrence ,progress and metastasis of the lung cancer ,and can be the evaluation indexes of disease and prognosis .%目的：探讨肺癌血管内皮生长因子（VEGF）及增殖细胞核抗原（PCNA）的表达及其与预后的关系。方法：采用免疫组织化学方法对62例肺癌组织及50例正常肺组织VEGF及PCNA进行检测，对比两组间VEGF及PCNA的表达及其与患者预后的关系。结果：肺癌组织VEGF及PC N A阳性率分别为67．7％和72．6％，显著高于正常肺组织的14．0％和12．0％（ P＜0．05）；肺癌VEGF阳性率与分化程度、淋巴转移、肿瘤大小及临床分期显著相关（P＜0．05）；PCNA 阳性率与分化程度、淋巴转移及临床分期显著相关（P＜0．05）；VEGF及PCNA阳性肺癌患者5年生存率显著低于阴性者（P＜0．05）。结论：VEGF及PCNA与肺癌的发生、进展及转移等生物学行为关系密切，可做为评估病情及预后的指标之一。

摘要： 植物体内的茉莉酸类物质是一类新确认的植物内源生长物质.本文概述了茉莉酸类物质的结构、生理生化作用以及相关分子生物的研究进展.

摘要： 本发明公开了一种PtTST1.1和PtTST2.1在制备促进植物生长物质中的应用。本发明方法简便；根据转基因杨树和转基因拟南芥的结果，PtTST1.1和PtTST2.1可以显著促进拟南芥和杨树的生长，对于培育速生高产的植物新品种具有重大基因价值。

摘要： 本发明属于肥料技术领域，特别是涉及一种含天然植物生长物质的肥料及其制备工艺，所述含天然植物生长物质的肥料的制备工艺包括如下步骤：S1：对粉碎后的海带进行低温分级浸提，将浸提液合并、浓缩；S2：将浸提后的海带加水依次用纤维素酶、果胶酶和岩藻糖苷酶进行分级酶解，得酶解液；S3：调节酶解液的pH值，与浸提浓缩液合并、浓缩，即得。本发明提供的含天然植物生长物质的肥料含有丰富的生物活性物质，能有效促进作物的生长发育；同时，本发明含天然植物生长物质的肥料的制备工艺简单易行，可有效提取和保留海带中的生物活性物质，生产成本低，生产工艺易于控制，有利于大规模工厂化生产。

摘要： 本发明是关于一种促毛发生长物质和其制备方法，以及以该促毛发生长物质为有效成份的促毛发生长剂及其应用，该促毛发生长物质是啤酒酵母(Saccharomyces cerevisiae Hansen)促毛1号CCTCC M201024菌株以Gibco1640培养基培养发酵后经除菌过滤所得的产物，其制备方法包括培养发酵、过滤除菌，该促毛发生长物质具有促进毛发生长作用，可用于治疗秃发和增加羊毛、兔毛等动物毛的产量，且安全无毒。

摘要： 本申请公开一种用于植物的冷冻切片方法，改进了固定液的配方，以及优化了包埋之前的处理，使得植物细胞在冷冻过程产生的冰晶减少，降低了冷冻过程对细胞造成的损伤，提高了植物组织切片的完整性。本申请还公开一种植物体内的植物生长物质免疫荧光定位方法，重点对抗原修复条件、抗体稀释度、孵育时间、洗涤方式以及抗体稀释液的选择进行优化，系统地建立了检测植物体内植物生长物质分布的免疫荧光定位方法，提高了植物体内植物生长物质免疫荧光定位的灵敏性和特异性,背景清晰,耗时少。

摘要： 本发明涉及一种壳聚糖包覆植物生长物质缓释微囊及其制备方法，属于植物生长物质、农药和农肥领域。本发明所述壳聚糖包覆植物生长物质缓释微囊为将植物生长物质用壳聚糖包覆后得到。在同等剂量条件下，采用壳聚糖包覆植物生长物质缓释微囊保持药效的时间更长。

摘要： 本发明属于肥料技术领域，特别是涉及一种含天然植物生长物质的肥料及其制备工艺，所述含天然植物生长物质的肥料的制备工艺包括如下步骤：步骤一，原料处理；步骤二、乳酸菌发酵；步骤三，超微粉碎；步骤四，复合酶酶解；步骤五，闪式提取；步骤六，离心分离；步骤七，微滤：将上清液用超滤膜过滤，纯化，得到滤液，即得含天然植物生长物质的肥料。本发明提供的含天然植物生长物质的肥料含有丰富的生物活性物质，能有效促进作物的生长发育；同时，本发明含天然植物生长物质的肥料的制备工艺简单易行，可有效提取和保留海带中的生物活性物质，生产成本低，生产工艺易于控制，有利于大规模工厂化生产。

摘要： 本发明公开了一种PtTST1.1和PtTST2.1在制备促进植物生长物质中的应用。本发明方法简便；根据转基因杨树和转基因拟南芥的结果，PtTST1.1和PtTST2.1可以显著促进拟南芥和杨树的生长，对于培育速生高产的植物新品种具有重大基因价值。

摘要： 本发明属于肥料技术领域，特别是涉及一种含天然植物生长物质的肥料及其制备工艺，所述含天然植物生长物质的肥料的制备工艺包括如下步骤：S1：对粉碎后的海带进行低温分级浸提，将浸提液合并、浓缩；S2：将浸提后的海带加水依次用纤维素酶、果胶酶和岩藻糖苷酶进行分级酶解，得酶解液；S3：调节酶解液的pH值，与浸提浓缩液合并、浓缩，即得。本发明提供的含天然植物生长物质的肥料含有丰富的生物活性物质，能有效促进作物的生长发育；同时，本发明含天然植物生长物质的肥料的制备工艺简单易行，可有效提取和保留海带中的生物活性物质，生产成本低，生产工艺易于控制，有利于大规模工厂化生产。

摘要： 一种复硝酚钠多微元素新型复合植物生长物质及其生产方法，本产品为固体或液体，其特征在于其成分有：复硝酚钠、锌盐、锰盐、铁盐、铜盐、钼酸盐、硼酸、镁盐、柠檬酸、氨水。本发明的配方为：复硝酚钠0.01－2.0、锌盐10－60、锰盐5－20、铁盐5－30、铜盐5－10、钼酸盐0.2－2.0、硼酸3－20、镁盐10－30、柠檬酸1－5、氨水1.0－30，其中微量元素可以是任何两种或两种以上。其优点为配方科学严密，工艺流程合理，生产方法简单，其生产出的产品对于农作物可促进植物生长发育、提高开花、打破休眠、促进发芽、防止落花落果、改良植物产品品质、使作物能够抗旱、抗涝、抗干热风、抗寒，是一种理想植物生长物质。

摘要： 本发明是关于一种促毛发生长物质和其制备方法，以及以该促毛发生长物质为有效成份的促毛发生长剂及其应用，该促毛发生长物质是啤酒酵母(Saccharomyces cerevisiaeHansen)促毛1号 CCTCC M201024菌株以Gibco1640培养基培养发酵后经除菌过滤所得的产物，其制备方法包括培养发酵、过滤除菌，该促毛发生长物质具有促进毛发生长作用，可用于治疗秃发和增加羊毛、兔毛等动物毛的产量，且安全无毒。