热休克蛋白质类70

摘要： 目的 构建pIRES2-MLAA34-HSP70重组质粒,并检测其免疫效果.方法 采用逆转录PCR(RT-PCR)的方法提取急性单核细胞白血病相关抗原基因MLAA-34和热休克蛋白(HSP) 70基因,设计特异性重叠引物,以重叠延伸PCR(SOE-PCR)技术扩增MLAA34-HSP70融合基因,构建核酸疫苗pIRES2-MLAA34-HSP70.将核酸疫苗免疫BALB/c小鼠,检测小鼠脾淋巴细胞对U937细胞的杀伤作用及小鼠脾细胞悬液中白细胞介素(IL)-2、IL-4和γ干扰素(IFN-γ)水平.结果 扩增出MLAA34-HSP70融合基因2 956 bp,成功构建了核酸疫苗pIRES2-MLAA34-HSP70,经鉴定与预期结果一致;脾淋巴细胞杀伤活性结果显示,核酸疫苗pIRES2-MLAA34-HSP70对U937细胞的杀伤效率明显高于其他实验组及对照组(P＜0.01);核酸疫苗pIRES2-MLAA34-HSP70组细胞因子IL-2、IL-4和IFN-γ水平亦明显高于其他实验组及对照组(P＜0.01).结论 成功构建了pIRES2-MLAA34-HSP70核酸疫苗,该核酸疫苗能诱发强烈的体液免疫,增强机体对肿瘤细胞的免疫应答,对MLAA34阳性细胞具有特异性杀伤作用.%Objective To construct the DNA vaccine pIRES2-MLAA34-HSP70,and to detect its immune effect.Methods The acute monocytic leukemia associated antigen gene MLAA-34 and heat-shock protein (HSP)70 gene were extracted by using RT-PCR.The specific overlapping primer was designed,and the fusion gene MLAA34-HSP70 was amplified by using SOE-PCR technique.Then the DNA vaccine pIRES2-MLAA34-HSP70 was constructed,and BALB/c mice were immunized with this DNA vaccine.The splenic lymphocyte killing activity was detected by using MTT,levels of IL-2,IL-4 and IFN-γ were also detected by using ELISA.Results The MLAA34-HSP70 gene (2 956 bp) and the DNA vaccine pIRES2-MLAA34-HSP70 was amplified and constructed successfully.The killing efficiency of DNA vaccine pIRES2-MLAA34-HSP70 in U937 cells was significantly higher than that in other experimental groups and control group (P＜0.01),and levels of IL-4,IL-2 and IFN-γin DNA vaccine pIRES2-MLAA34-HSP70 group were significantly higher than those in the other experimental groups and control group (P＜0.01).Conclnsion The DNA vaccine pIRES2-MLAA34-HSP70 is constructed successfully.It is shown that the DNA vaccine induces strong humoral immunity,which could enhance immune responses to tumor cells and specificlly kill MLAA34 positive cells.

摘要： Objective To investigate the dynamic change of paraquant-induced kidney injury in rats and the protective effect of edaravone. Methods Eighty SD rats were randomly divided into 4 groups: the normal control group, paraquat poisoning group, edaravone treatment group and edaravone control group. The normal control group of 8 rats were given 1 ml of 0.9% sodium chloride through the abdominal cavity , and the same amount of fluid into the abdominal cavity after 30 minutes. The paraquat poisoning group of 24 rats were given 1 ml of paraquat solution(20 mg/kg) through the abdominal cavity to build poisoning models, and the same amount of 0.9%sodium chloride was injected into the abdominal cavity after 30 minutes . The edaravone treatment group of 24 rats were given edaravone (5 mg/kg) through the abdominal cavity after 30 minutes when the poisoning models were set up. The edaravone control group of 24 rats were given 1 ml of 0.9% sodium chloride through the abdominal cavity,and edaravone(5 mg/kg) was injected into the abdominal cavity after 30 minutes. In addition to the normal control group, the other groups processed 1 times a day to mantain 7 d. On 1, 3, 7, 21 d several rats in each group were excuted and the kidney tissue and serum samples were collected , then each pathological changes of the kidney were observed with light microscopy. Serum creatinine , KIM-1, NGAL were measured by ELISA, the expression of HSP70 protein in kidney were observed with immunohistochemical staining. Results The pathological examination reveald that the damage of kidney tissue in the paraquat group was the most serious on 3 d, and the damage was consistently alleviated in edaravone treatment group at the same time ,renal fibrosisn was unseen in each group until 21 d. Compared with normal control group,there was no statistically significant in edaravone control group (P>0.05). The KIM-1 in blood and kidney in paraquat poisoning group were markedly increased in 1 d(P0.05).Compared with paraquat poisoning group, the serum creatinine, KIM-1 in blood and kidney, the KIM-1 in kidney had decreased significantly in edaravone treatment group (P0.05). HSP70 expression of kidney tissue in edaravone treatment group had significantly increased in d3 compared with the paraquat poisoning group (P0.05).与百草枯中毒组相比,依达拉奉治疗组大鼠血肌酐、KIM-1、NGAL和肾脏KIM-1表达下降(P0.05).与百草枯中毒组比较,依达拉奉治疗组第3、21天大鼠肾脏组织中HSP70表达明显增加(P<0.05).结论 依达拉奉可促使肾脏组织HSP70表达升高,降低KIM-1和NGAL水平,对急性百草枯中毒所致的肾损伤起到一定保护作用.

摘要： Objective To study the correlation of heat shock protein 70-hom (Hsp70-hom) gene polymorphisms with susceptibility to cerebral infarction and hypertension in the elderly. Methods Two hundred and twenty elderly patients were divided into cerebral infarction group(n=105) and hypertensin group(n= 115). Patients in cerebral infarction group were further divided into simple cerebral infarction group (n — 38) and cerebral infarction plus hypertension group (n= 67). One hundred healthy subjects served as a control group. Hsp70-hom +2437 gene polymorphisms were detected by PCR-RFLP. Results The proportion of Hsp70-hom TT genotypes and T alleles was significantly higher in cerebral infarction group than in control group(70. 48%,82. 38%, OR = 3. 311,95%CI:2. 078 — 4. 359, P = 0. 000 ;OR = 2. 121, 95% CI:A. 271 — 3. 325, P=0. 003). The proportion of C and T alleles was significantly different among cerebral infarction plus hypertension group,simple cerebral infarction group, and control group(OR = l. 193,95%CI:1. 128 — 3. 246,P<0. 05 ;OR = 2. 312,95%C7:1. 128-4. 721, P<0. 05). Conclusion Hsp70-hom gene polymorphisms are correlated with the susceptibility to cerebral infarction. The value of detecting Hsp70-hom + 2437 genotypes should be further studied.%目的 探讨热休克蛋白70(Hsp70)-hom基因多态性与老年脑梗死和高血压发生的关系.方法 选择住院和门诊的老年患者220例,按诊断分为脑梗死组105例和高血压组115例,脑梗死组又分为单纯组38例和合并组67例,同期选取健康体检者100例为健康组.采用PCR-RFLP方法检测Hsp70-hom +2437位点基因多态性.结果 与健康组比较,脑梗死组Hsp70-hom TT基因型及T等位基因明显升高,分别达到70.48％和82.38％(OR=3.311,95％CI:2.078～4.359,P=0.000;OR=2.121,95％CI:1.271～3.325,P=0.003).与健康组比较,合并组、单纯组C与T等位基因频率有显著差异(OR=1.193,95％CI:1.128～3.246,P＜0.05;OR=2.312,95％CI:1.128～4.721,P＜0.05).结论 Hsp70-hom基因多态性与脑梗死易感性有关,Hsp70-hom+2437位点基因型的检测价值,值得进一步探讨.

摘要： Objective To investigate the dynamic expression of Heat shock protein 70 (Hsp70) in the lungs and plasma of rats with pulmonary fibrosis induced by silicon dioxide (SiO2).Methods Forty-eight Wistar rats were randomly divided into the control group exposed to normal solution and group exposed to SiO2 (50 mg/ml) with intratracheal injection.Each group was divided into four subgroups.The animals of SiO2 group and control group were sacrificed and lungs were collected on the 7th,14th and 28th days after exposure,respectively.The left lung tissues were examined with the histopathologic HE s taining.The expression and localization of Hsp70 protein in the lung tissues were examined with western blot assay and immunohistochemistry,respectively.The expression levels of Hsp70 protein in the plasma were measured by ELISA.Results The expression of Hsp70 in lung tissues of SiO2 group increased on the 7th day and reached the peak value on the 14th day then decreased,but still was significantly higher than that of the control group,the expression of Hsp70 in plasma of SiO2 group still was significantly higher than that of the control group (P＜0.05).The maximum expression level of Hsp70 in plasma of SiO2 group on the 21st day after exposure was 0.216 ± 0.027 μg/ml.Conclusion The expression levels of Hsp70 protein in the lung tissues and plasma of the group exposed to SiO2 significantly increased,which were associated with the process of pulmonary fibrosis.It was suggested that Hsp70 protein may play an important biological role in the pulmonary fibrosis induced by SiO2.%目的 探讨热休克蛋白70( heat shock protein 70,Hsp70)在二氧化硅诱导的大鼠肺纤维化过程中的变化.方法 48只Wistar大鼠随机分成对照组和染尘组,每组24只,分别观察7、14、21、28d.染尘组经气管内灌注二氧化硅(50mg/ml),对照组予等剂量的生理盐水.HE染色观察病理学变化,蛋白免疫印迹( Western bolt)法和免疫组织化学法检测肺组织中Hsp70的表达与定位,双抗夹心ELISA检测血浆中Hsp70含量的变化.结果 染尘组染尘后7d肺组织中Hsp70含量升高,14d达到高峰,随后降低,但仍高于正常对照.染尘组14、21、28 d时血浆中Hsp70含量明显高于生理盐水对照组,差异有统计学意义(P＜0.05).染尘组血浆中Hsp70含量21d达到峰值[(0.216±0.027) μg/ml].结论 在染尘组大鼠肺组织和血浆中Hsp70含量均明显升高,并随肺纤维化进程出现先升高后降低的趋势,提示肺组织中Hsp70在二氧化硅诱导的肺纤维化过程中有重要的生物学作用.

摘要： @@ 热休克蛋白(HSPs),也称应激蛋白,是一类结构上高度保守的蛋白,广泛存在于原核生物和真核生物.多数HSPs因环境改变而被诱导,其中HSP70家族在生物细胞中含量最高,可诱导性最强.

摘要： Objective To observe the expression levels of heat shock protein70 (hsp70) and NF-κB p65 mRNA in lung tissue of acute paraquat (PQ) poisoning rats, and intervention effects of ulinastatin (UTI).Methods Seventy-two Sprague-Dawley (SD) rats were randomly divided into three groups: PQ poisoning group, UTI group and control group. The rats were exposed intragastrically to PQ at the dose of 80 mg/kg to establish a model of the rat acute lung injury. The UTI group was intervened by peritoneal injection with 10000 U/kg UTI in 30 minutes. On the 12, 24, 48, 72 h after exposure, myeloperoxidase (MPO) activity in lung tissue were detected. The expression of the NF-κBp 65 mRNA and hsp70 mRNA in lung tissue was detected by the reverse transcription-PCR (RT-PCR).The lung pathological changes of rats were observed. Results The degree of lung injury in PQ group and UTI group was higher than that in control group. But in UTI group the degree of lung injury was lower than PQ group. MPO activity in the lung tissues in PQ group was (31.72±6.42),(56.23±8.63), (87.21 ±10.02) and (107.21 ±13.52) μ/g in 12, 24, 48 and 72 h, respectively which was significantly higher than that [(11.38±1.25) μ/g] in control group (P＜0.01). MPO activity in the lung tissues in UTI group was (15.65±3.21), (35.98±5.74), (59.33±9.65) and (71.25±10.58) μ/g in 12, 24, 48 and 72 h,respectively which was significantly lower than those in PQ group (P＜0.01). The expression levels of NF-κB p65 mRNA of lung tissues in UTI group in 12, 24, 48 and 72 h were 0.3288±0.0147, 0.5337±0.0328, 0.7357±0.0424 and 0.7547±0.0905, respectively, which were significantly lower that those (0.4185±0.0294, 0.8532±0.0841,0.9554±0.0975 and 1 .0094±0.0703) in PQ group (P＜0.01). hsp70 mRNA expression levels in 12, 24,48 and 72 h of the UTI group were 0.5193±0.0254, 0.8289±0.0606, 0.7566±0.0277 and 0.4873±0.0105,respectively, which were significantly higher than those (0.3897±0.0125, 0.5904±0.0186, 0.4007±0.0237 and 0.2293±0.0137) in PQ group (P＜0.01). Conclusion The expression levels of hsp70 mRNA and NF-κB p65 mRNA of rats after intoxication increased significantly. UTI can protect the lung tissus by elevating the expression of hsp70 and reducing the expression of N F-κB in the lung tissues of rats with acute paraquat poisoning.%目的 观察急性百草枯(paraquat,PQ)染毒大鼠肺组织中热休克蛋白70(hsp70)及NF-κB mRNA表达及乌司他丁(ulilnastatin,UTI)的作用.方法 将72只SD大鼠随机分为对照组、PQ组和UTI组,每组24只.染毒组:以80 mg/lg PQ一次性灌胃;治疗组:PQ灌胃后以UTI 10000 U/kg腹腔注射,每日1次.对照组一次性灌胃生理盐水.于12、24、48、72 h分别测定肺组织中髓过氧化物酶(MPO)活力,反转录-聚合酶链反应(RT-PCR)法测定肺组织中hsp70和NF-κB p65 mRNA的表达,观察大鼠肺组织病理变化.结果 PQ染毒后48 h内炎性细胞浸润明显,伴充血水肿;UTI组炎性细胞浸润较PQ染毒组少,充血水肿轻.染毒组大鼠肺组织中MPO活力在12、24、48、72 h分别为(31.72±6.42)、(56.23±8.63)、(87.21±10.02)、(107.21±13.52)U/g,UTI组12、24、48、72 h MPO活力分别为(15.65±3.21)、(35.98±5.74)、(59.33±9.65)、(71.25±10.58)U/g,较对照组[(11.38±1.25)U/g]明显升高,差异有统计学意义(P＜0.01);UTI组MPO活力较染毒组明显降低,差异有统计学意义(P＜0.01).UTI组12、24、48、72 h NF-κB p65 mRNA表达分别为0.3288±0.0147、0.5337±0.0328、0.7357±0.0424、0.7547±0.0905,与PQ组(分别为0.41 85±0.0294、0.8532±0.0841、0.9554±0.0975、1.0094±0.0703)比较,明显降低,差异有统计学意义(P＜0.01).UTI组12、24、48、72 h hsp70 mRNA表达分别为0.5193±0.0254、0.8289±0.0606、0.7566±0.0277、0.4873±0.0105,与PQ组(分别为0.3897±0.0125、0.5904±0.0186、0.4007±0.0237、0.2293±0.0137)比较明显增加,差异有统计学意义(P＜0.01).结论 大鼠急性PQ中毒后肺组织中hsp70、NF-κB p65 mRNA表达明显增高;UTI可以通过增加PQ中毒大鼠肺组织中HSP70表达,降低NF-κB表达,对肺脏起保护作用.

摘要： Objective To investigate the protective role of inducible heat shock protein 70 (Hsp70) against damage induced by formaldehyde. Methods Human bronchial epithelium (HBE) cells were transfected with plasmid harboring hsp70 gene to increase the protein expression level. HBE cells transfected with pcDNA3.1 plasmid were used as transfection control and HBE cells cultured at normal condition served as control. Three groups were marked as HBE/hsp70, HBE/pcDNA and HBE. Hsp70 expression levels of 3 groups were detected. The cells of HBE/hsp70 and HBE groups were exposed to different concentrations of formaldehyde (0,0.39,1.56,6.25 mmol/L) for 4 h. The contents of GSH and MDA were measured, and KC1-SDS method was applied to measure DNA-protein crosslink (DPC). Results Hsp70 level in HBE/hsp70 group increased by 80% compared with HBE group. GSH contents in HBE/hsp70 group significantly increased and were 141.0, 119.6 mg/gpro at 0.39, 1.56 mmol/L, respectively (P<0.01), as compared with HBE group. However, it decreased when formaldehyde concentration increased to 6.25 mmol/L. While GSH content in HBE group remained decreasing. MDA contents in HBE/hsp70 and HBE group increased with formaldehyde. MDA content in HBE/hsp70 was 0.088 μmol/gpro and significantly lower than that (0.138 μmol/gpro) in HBE group (P<0.05) when formaldehyde concentration was 1.56 mmol/L, At the formaldehyde dose of 6.25 mmol/L MDA content in HBE/hsp70 was 0.140 μmol/gpro which was significantly lower than that (0.289 μmol/gpro) in HBE group (P<0.01). DPC% in two groups increased with formaldehyde. At the formaldehyde dose of 0.39 mmol/L, DPC% in HBE/hsp70 group was 3.94% which was significantly lower than that (6.25%) in HBE group (P< 0.01). At the formaldehyde dose of 1.56 mmol/L, DPC% in HBE/hsp70 group was 11.86% which was significantly lower than that (20.89% ) in HBE group (P<0.05). Conclusion Hsp70 can reduce formaldehyde-induced damages in human bronchial epithelium cells in vitro.%目的 研究诱导型热休克蛋白70(heat shock protein 70,Hsp70)在甲醛所致损伤中的保护作用.方法 人支气管上皮细胞human bronchial epithelium,HBE)转染含有hsp70基因的质粒,以增高Hsp70的表达,转染载体质粒的细胞和正常培养的细胞作为对照,分别为Hsp70高表达组(HBE/hsp70)、转染对照组(HBE/pcDNA)和对照组(HBE),并对3组细胞Hsp70的表达量进行鉴定.HBE/hsp70组和HBE组在接受不同浓度甲醛(0、0.39、1.56、6.25 mmol/L)染毒4 h后,检测各组细胞谷胱甘肽(GSH)和丙二醛(MDA)含量以及DNA-蛋白质交联(DNA-protein crosslink,DPC).结果 细胞转染含有hsp70基因的质粒后,Hsp70蛋白表达量与HBE组相比增高约80%.HBE/hsp70组GSH含量先增高,在甲醛浓度为0.39和1.56 mmol/L时分别为141.0、119.6 mg/gpro,与HBE组(分别为75.8、56.7 mg/gpro)比较,差异有统计学意义(P<0.01),但当甲醛浓度增高到6.25 mmol/L时,GSH含量降低.而HBE组细胞的GSH含量随着甲醛浓度的增高一直呈下降的趋势.HBE/hsp70组、HBE组细胞MDA含量均随甲醛浓度的升高而增加,且HBE/hsp70组细胞MDA含量一直低于HBE组,在甲醛浓度为1.56 mmol/L时MDA含量为0.088μmol/gpro,与HBE组(0.138 μmol/gpro)相比,差异有统计学意义(P<0.05),甲醛浓度为6.25 mmol/L时,HBE/hsp70组细胞MDA含量为0.140 μmol/gpro,与HBE组(0.289 μmol/gpro)相比,差异有统计学意义(P<0.01).HBE/hsp70组、HBE组细胞DPC百分含量均随甲醛浓度的升高而增加,且HBE/hsp70组一直低于HBE组,在甲醛浓度为0.39 mmol/L时,HBE/hsp70组DPC含量为3.94%,HBE组为6.25%,差异有统计学意义(P<0.01),甲醛浓度为1.56 mmol/L时,HBE/hsp70组DPC含量为11.86%,HBE组为20.89%,差异有统计学意义(P<0.05).结论 Hsp70可降低甲醛对体外支气管上皮细胞造成的损伤.

摘要： 目的 研究亚慢性染毒苯并[a]芘(B[a]P)对大鼠海马热休克蛋白70( Hsp70)的影响。方法 选择48只健康雄性SD大鼠，饲养1周后将动物随机分为空白对照组、溶剂对照组及0.5、1.5、4.5、10.0 mg/kg B[a]P染毒组，隔日腹腔注射染毒90d，Morris水迷宫试验进行行为学测试。免疫印迹(Westem-blot)法和反转录-聚合酶链反应(RT-PCR)法分别检测大鼠海马Hsp70蛋白和Hsp70 mRNA的表达水平，免疫组织化学法观察大鼠海马各区Hsp70表达情况，图像分析系统测定Hsp70平均灰度值。结果 Morris水迷宫结果显示，4.5、10.0 mg/kg B[a]P组大鼠平均潜伏期和平均总路程明显高于空白对照组、溶剂对照组、0.5、1.5 mg/kg B[a]P组，差异有统计学意义(P＜0.05)；10.0 mg/kg B[a]P组平台象限滞留时间明显低于空白对照组、溶剂对照组和0.5mg/kg B[a]P组，差异有统计学意义(P＜0.05)。B[a]P染毒组大鼠海马hsp70蛋白和hsp70 mRNA的表达水平均随染毒剂量增高而下降。其中，4.5、10.0 mg/kgB[a]P组Hsp70蛋白表达水平明显低于空白对照组、溶剂对照组和0.5mg/kg B[a]P组，差异有统计学意义( P＜0.01，P＜O.05)；0.5、4.5、10.0 mg/kg B[a]P组hsp70 mRNA表达水平明显低于空白对照组、溶剂对照组，差异有统计学意义(P＜0.01)，10.0 mg/kg B[a]P组hsp70 mRNA表达水平明显低于其他染毒组。免疫组化结果显示，海马CA1、CA2、CA3、DG区Hsp70表达平均灰度值均随着B[a]P染毒剂量的增高而呈下降趋势。Pearson相关性分析结果显示，大鼠海马Hsp70表达水平与平均潜伏期呈负相关（r=-0.428，P＜O.05）；大鼠海马hsp70 mRNA表达水平与平均潜伏期、平均总路程呈负相关(r值分别为-0.677、-0.594，P＜0.01)，与平台象限滞留时间呈明显正相关(r=0.597,P＜O.01)。结论 亚慢性染毒B[a]P可抑制大鼠海马Hsp70的表达，与大鼠学习记忆和空间探索能力损害相关，该抑制作用无区域特异性。%Objective To study the influence of sub-chronically exposure to benzo[a]pyrene (B[a]P)on heat shock protein 70 (Hsp70) expression in rat hippocampus. Methods Forty eight healthy adult male SD rats were randonly divided into 6 groups: blank control, olive control and four B[a]P-treated groups, which were exposed intra-peritoneally to B[a]P at the doses of 0.5, 1.5, 4.5 and 10.0 mg/kg for 90 days. The learning and memory function was measured by Morris water maze. The expression level of Hsp70 and hsp70 mRNA in rat hippocampus were detected by Western-blot and RT-PCR, respectively. Immunohistochemistry was used to examine the Hsp70 expression levels in hippocampus CA1, CA2, CA3, DG regions, which were reflected by average gray values of Hsp70 immunoreactive products measured by image analysis system. Results The results of Morris water maze test showed the escape latencies and total distances of navigation in 4.5 and 10.0 mg/kg B[a]P groups were significantly higher than those in two control groups and 0.5 or 1.5 mg/kg B[a]P groups (P＜0.05).The duration of the third quadrant of the space search experiment in 10.0 mg/kg B[a]P group was significantly lower than those in two control groups and 0.5 mg/kg B[a]P group (P＜0.05). The expression levels of Hsp70 and hsp70 mRNA in rat hippocampus decreased with the B[a]P dose. The expression levels of Hsp70 in 4.5 and 10.0 mg/kg B[a]P groups were significantly lower than those of two control groups and 0.5 mg/kg B[a]P group (P＜0.05 or P＜0.01 ). The expression levels of hsp70 mRNA in 0.5, 4.5 and 10.0 mg/kg B[a]P groups were significantly lower than those in two control groups (P＜0.01) and the expression level of hsp70 mRNA in 10.0 mg/kg B[a]P group was significantly lower than those in other B[a]P groups. The results of immunohistochemistry examination indicated that the average gray values of Hsp70 immunoreactive products in CA1, CA2, CA3, DG regions decreased with dose of B[a]P. Pearson correlation analysis showed that there was a negative correlation between the expression of Hsp70 protein in hippocampus and the average escape latency (r=-0.428, P＜0.05). There was a negative correlation between the expression of hsp70 mRNA in hippocampus and the average escape latency,the total distance of the third quadrant (r=--0.677 and r=-0.594, P＜0.01 ). There was a positive correlation between the expression of hsp70 mRNA in hippocampus and the average total duration of the third quadrant (r=0.597, P＜0.01). Conclusion Sub-chronic exposure to B[a]P could inhibit the expression of Hsp70 in rat hippocampus, which was related to the ability of learning and memory of rats and the inhibition effects did not have the region-specificity.

摘要： @@ 肝细胞癌(HCC)是我国的常见恶性消化道肿瘤之一,全球发病率逐年升高,其发生和发展与遗传、免疫和环境等因素相关.热休克蛋白(HSP)70是生物细胞受到高温、感染、炎症和缺血等应激刺激时产生的一组在进化上高度保守的蛋白质,具有广泛的生物学功能,与HCC的发病关系密切.

摘要： 目的 研究微波暴露对大鼠海马脑区热休克蛋白(HSP)70表达的变化,为阐明微波的生物效应与机制提供线索.方法 采用峰值功率密度为90、5 W/cm2的微波全身一次性辐照大鼠20min,采用反转录-聚合酶链反应(RT-PCR)法观察微波辐照后不同时相点大鼠海马脑区hsp70 mRNA表达的变化;采用免疫印迹(Western-blot)法观察微波辐照后HSP70蛋白水平的变化.结果 90、5W/cm2微波辐照后,大鼠的肛温[分别为(40.40±0.19)、(38.22±0.68)°C]以及比吸收率(SAR)值[分别为(15.09±0.81)、(5.56±0.31)W/kg]明显升高.2个微波暴露组在20 min暴露后均可见hsp70 mRNA和蛋白水平表达上调.结论 微波辐照有明显的热效应,是hsp70合成极为敏感的诱因,并可能启动了脑的内源性保护机制.%Objective To study the change of heat shock protein(HSP)70 expression after exposure to occupational microwave in rats hippocampus, and explore the role of HSP70 in the mechanism of bio-effect of microwave irradiation. Methods The animal model was established by whole body exposures in 90, 5 W/cm2 microwave irradiation field for 20 min in rats. Changes of the mRNA of hsp70 expressions in rat hippocampus at different time were studied by RT-PCR, and the protein change by Western blot. Results The mRNA and protein expression of hsp70 in rat hippocampus increased after 90 W/cm2 and 5 W/cm2 microwave irradiation for 20 min. The anal temperature and the value of SAR increased significantly. These changes were positively correlated with power and irradiation time of microwave. The results indicated that microwave irradiation led to HSP70 syntheses effectively. Conclusion Microwave irradiation can obviously induce the thermal effect and activate HSP70, and initiate the endogens protective mechanism of central nervous system.

摘要： 本发明公开了一种用于在细胞和组织中刺激热休克蛋白的活化和促进蛋白质修复的系统和方法，以利用增加了的热休克蛋白的活化或产生以及细胞修补的促进的重新介导和恢复性质，而不损坏细胞和组织。这是通过脉冲且施加到或聚焦到一个或多个小的区域的超声波源或电磁辐射源来治疗指定的目标区域，以实现必要的温度的升高或者实现充分地应激细胞和组织，以刺激热休克蛋白的产生或活化以及促进蛋白质修复，同时允许温度足够快地衰减以免损害或破坏待治疗的组织。

摘要： 本发明涉及一种含有sHSPs用于防止蛋白质降解的组合物，以及一种用于二维凝胶电泳的组合物。此外，本发明还涉及一种改进的二维凝胶电泳方法，其特征在于使用了sHSPs。根据本发明，可防止二维凝胶电泳中的蛋白质斑点减少，从而获得具有大量蛋白质斑点的二维凝胶。

摘要： 本发明提供用HSP90抑制剂，特别是HSP90β抑制剂，治疗代谢综合征的方法。本发明提供使用HSP90(特别是HSP90p)的表达和活性水平诊断和监测代谢综合征的方法。

摘要： 本发明公开了一种用于在细胞和组织中刺激热休克蛋白的活化和促进蛋白质修复的系统和方法，以利用增加了的热休克蛋白的活化或产生以及细胞修补的促进的重新介导和恢复性质，而不损坏细胞和组织。这是通过脉冲且施加到或聚焦到一个或多个小的区域的超声波源或电磁辐射源来治疗指定的目标区域，以实现必要的温度的升高或者实现充分地应激细胞和组织，以刺激热休克蛋白的产生或活化以及促进蛋白质修复，同时允许温度足够快地衰减以免损害或破坏待治疗的组织。

摘要： 本发明涉及一种含有sHSPs用于防止蛋白质降解的组合物，以及一种用于二维凝胶电泳的组合物。此外，本发明还涉及一种改进的二维凝胶电泳方法，其特征在于使用了sHSPs。根据本发明，可防止二维凝胶电泳中的蛋白质斑点减少，从而获得具有大量蛋白质斑点的二维凝胶。

摘要： 本发明的课题在于提供鉴别对公知的HSP90抑制剂呈耐性的患者的方法、以及用于治疗对公知的HSP90抑制剂呈耐性的患者的新的治疗药。本发明中，作为解决上述课题的手段，提供一种具有突变的蛋白质，其为HSP90家族蛋白质，该具有突变的蛋白质在相当于由序列号1的氨基酸序列构成的HSP90αA类的F138的部位具有突变，基于该具有突变的蛋白质，对患者进行鉴别，并将抑制该蛋白质的物质作为治疗药的有效成分。

摘要： 本发明提供用HSP90抑制剂，特别是HSP90β抑制剂，治疗代谢综合征的方法。本发明提供使用HSP90(特别是HSP90p)的表达和活性水平诊断和监测代谢综合征的方法。

摘要： 由下述氟树脂形成了与蛋白质或包含蛋白质的组合物接触的表面的容器及用于制造蛋白质或包含蛋白质的组合物的器材具有显著的低蛋白质吸附性，其中，所述氟树脂为选自四氟乙烯‑六氟丙烯系共聚物及四氟乙烯‑全氟烷基乙烯基醚系共聚物中的至少1种氟树脂，并且，所述氟树脂的熔点为320°C以下，所述氟树脂中的相对于每1×10

摘要： 本发明提供能减小使用了特异性地结合的物质的测定中的由肝型脂肪酸结合蛋白质引起的测定值的变动幅度的肝型脂肪酸结合蛋白质制剂、评价该制剂的方法、制作肝型脂肪酸结合蛋白质的校准曲线的方法、以及定量该蛋白质的方法。一种用使用了用10mM的氧化剂在25°C下进行1小时的氧化处理的肝型脂肪酸结合蛋白质制剂的测定值与使用了未进行上述氧化处理的肝型脂肪酸结合蛋白质制剂的测定值的比表示的氧化变动系数设定为1.4以下的肝型脂肪酸结合蛋白质制剂、用于该制剂的肝型脂肪酸结合蛋白质、编码该蛋白质的DNA、由该DNA转化得到的细胞、该蛋白质的制造方法、评价该制剂的方法、抑制使用该制剂的测定中的测定值的变动幅度的方法、制作该蛋白质的校准曲线的方法、以及定量该蛋白质的方法。

摘要： 本发明提供一类能够携带生物活性蛋白分子穿透细胞膜的非肽类多胍有机小分子或其药学上可接受的盐，所述非肽类多胍有机小分子具有式I所示的结构：