您现在的位置: 首页> 研究主题> 溴化乙锭

溴化乙锭

溴化乙锭的相关文献在1994年到2022年内共计96篇,主要集中在化学、生物化学、肿瘤学 等领域,其中期刊论文79篇、会议论文7篇、专利文献10篇;相关期刊58种,包括聊城大学学报(自然科学版)、国际检验医学杂志、中国实验诊断学等; 相关会议7种,包括2009年中国中西部地区无机化学、化工学术研讨会、全国第十四届大环化学暨第六届超分子化学学术讨论会、第十二届全国稀土元素分析化学学术报告暨研讨会等;溴化乙锭的相关文献由321位作者贡献,包括宋玉民、李东辉、李来生等。

溴化乙锭—发文量

期刊论文>

论文:79 占比:82.29%

会议论文>

论文:7 占比:7.29%

专利文献>

论文:10 占比:10.42%

总计:96篇

溴化乙锭—发文趋势图

溴化乙锭

-研究学者

  • 宋玉民
  • 李东辉
  • 李来生
  • 鄢远
  • 黄伟东
  • 刘晔
  • 向蓉
  • 张岐
  • 王海燕
  • 黄菊
  • 期刊论文
  • 会议论文
  • 专利文献

搜索

排序:

年份

    • 张杰; 李军生; 覃懿; 黄国霞; 阎柳娟; 马纪
    • 摘要: 文章利用紫外吸收光谱研究了DNA与溴化乙锭(EtBr)的相互作用、散射共振光谱研究了二者的相互作用强度及影响因素,最后通过磁珠分离EtBr-DNA复合物以去除EtBr污染物。紫外可见吸收光谱结果表明DNA与EtBr的结合模式为.嵌插结合。散射共振光谱和EtBr去除实验结果表明,CaCl_(2)、SDS、CTAB、葡萄糖和尿素能够促进二者的结合,使DNA结合饱和值变大,提高EtBr的去除效率;NaCl抑制二者的结合,使DNA结合饱和值变小,降低EtBr的去除效率。研究为高效去除EtBr污染物提供新见解。
    • 郑庆伟
    • 摘要: 南京农业大学植物保护学院以昆虫细胞Sf9为材料,利用Cell Counting Kit-8(CCK-8)试剂盒,检测了对多种昆虫具有高毒力的球孢白僵菌菌株NJBb2101发酵液产物对细胞增殖的抑制作用;利用AO/EB(吖啶橙-溴化乙锭)染色和Annexin V-FITC/PI(Annexin V-fluorescein isothiocyante/propidium iodide)染色-流式细胞仪2种检测方法,测定了高、低质量浓度(1.92和0.48mg/mL)发酵液产物对细胞凋亡的诱导作用。
    • 何熠; 衡淑君; 郑立
    • 摘要: 目的:研究低剂量溴化乙锭对大鼠骨髓来源间充质干细胞(BMSC)中线粒体DNA(mtDNA)的影响,建立低含量mtDNA的BMSC模型.方法:实验分为对照组(细胞未处理)、C-49d组(50ng/mL溴化乙锭处理49d+100μg/mL丙酮酸钠+50μg/mL尿嘧啶)及无尿嘧啶C-49d组(50ng/mL溴化乙锭处理49d).于倒置显微镜下观察各组BMSC的形态,测定细胞活力,采用实时荧光定量PCR(qPCR)法检测对照组和C-49d组mtDNA相关基因的表达情况.结果:与对照组比较,C-49d组细胞生长速度缓慢,细胞轮廓变大,呈现多角状,较之对照组更为短且扁平.对照组细胞活力高于C-49d组和无尿嘧啶C-49d组(P<0.05),无尿嘧啶C-49d组细胞活力低于C-49d组(P<0.05).C-49d组线粒体相关基因ND1、ND2、ND3、 COX1、COX2、COX3及CYTB表达量均显著低于对照组(均P<0.05).结论:低剂量溴化乙锭处理可抑制BMSC中mtDNA的含量,成功构建低含量mtDNA的BMSC模型.%Objective:To investigate the effect of low-dose ethidium bromide on mitochondrial DNA (mtDNA) of bone mesenchymal stem cells(BMSC).Methods:BMSC was divided into control group,C-49d group(50ng/mL ethidium bromide+50μg/mL sodium pyruvate+50μg/mL uracil) and non-uracil C-49 d group(50ng/mL ethidium bromide).The morphology of BMSC was observed and the cell viability was measured.The expression of mitochondrial protein coding genes was detected by real-time fluorescence quantitative PCR(qPCR).Results:The C-49dgroup cells presented polyhedral shape,slower growth,shorter and flatter than the controls.The cell viability of the control group was higher than that of the C-49d group and non-uracil C-49dgroup(P<0.05).Non-uracil C-49dgroup had lower cell viability than the C-49 dgroup (P<0.05).The expression of mitochondrial protein coding genes(ND1,ND2,ND3,COX1, COX2,COX3and CYTB) in the C-49dgroup was significantly lower than that in the control group(P<0.05).Conclusion:Low-dose of ethidium bromide could inhibit the expression of mtDNA in BMSC,and successfully construct mtDNA-less BMSC model.
    • 马强; 蔡燕; 徐磊; 廖何斌; 邹江; 孙茹; 郭晓兰
    • 摘要: Objective To explore the causes of twisted or tailed DNA bands in agarose gels after electro-phoresis .Methods Prestained and poststained methods were employed to exam 3 kinds of DNA marker bands in agarose gel with GeneGreen and Ethidium Bromide ,respectively .Results Three kinds of DNA marker bands in agarose gel with 1:10000 and 1:5000 concentration of GeneGreen showed obvious distortions and trailing phenomena compared with the same concentration of Ethidium Bromide w hen the prestained method was used for electrophoresis ,which were improved when the poststained method was used in agarose gel with GeneGreen and Ethidium Bromide ,respectively .Conclusion Nucleic acid dye GeneGreen can affect the quali-ty of DNA bands in agarose gel electrophoresis .When the quality of DNA itself ,agarose gel quality ,electro-phoretic fluid quality and voltage are excluded ,the quality of nucleic acid dye should be taken into considera-tion if the bands twisting or tailing occurs when the DNA nucleic acid electrophoresis is carried out by pres-tained method .The quality of DNA electrophoresis bands can be improved by using poststained method or re-placing nucleic acid dye .%目的 探讨DNA琼脂糖凝胶电泳条带扭曲、"拖尾"的原因.方法 采用"胶染法"和"后染法"2种方法,检测含2种核酸染料(GeneGreen和溴化乙锭)的琼脂糖凝胶中3种DNA Marker条带形态.结果采用"胶染法"的方式电泳,与相同浓度的溴化乙锭相比,含1:10000和1:50002种浓度的GeneGreen琼脂糖凝胶中3种DNA Marker条带呈现明显的扭曲和"拖尾"现象.而采用"后染法"的方式后,两种核酸染料所染的琼脂糖凝胶中3种Marker的条带均呈单一、无扭曲和"拖尾"现象.结论 核酸染料GeneGreen可以影响琼脂糖凝胶电泳时DNA条带的质量.在排除DNA 本身质量、琼脂糖凝胶质量、电泳液质量以及电压等因素后,若采用"胶染法"进行DNA核酸电泳时出现条带扭曲或"拖尾"时,应考虑到核酸染料质量的问题.采用"后染法"或更换核酸染料的种类可改善DNA电泳条带的质量.
    • 杨丽艳; 王竹; 刘圆圆; 徐克前
    • 摘要: Objective To investigate the DNA staining efficiencies of 4 kinds of nucleic acid fluorescent dyes,including EB,SYBR Green Ⅰ,Gold View and AO,in single-cell gel electrophoresis (SCGE) assay,and the possibility to use a new nucleic acid fluorescent dye instead of EB.Methods The peripheral blood lymphocytes from healthy individuals were isolated and treated with 0,20,40,60 and 80 μg/mL of H2O2,respectively.Then,the DNA damages of lymphocytes were detected by the neutral SCGE assay.The DNA was stained with EB,SYBR Green Ⅰ,Gold View and AO dyes,respectively,and the staining results were observed and compared under a fluorescence microscope.In addition,the percentages of tail DNA (% Tail DNA) from different staining methods were analyzed and compared.Results The results of SCGE showed that SYBR Green Ⅰ,Gold View and AO staining could well reflect the DNA damages of lymphocytes,and that the optimal concentrations for SYBR Green Ⅰ,Gold View and AO were 1 ×-5 ×,2 ×-5 × and 2-5 μg/mL,respectively.The regression coefficients for EB,SYBR Green Ⅰ,Gold View and AO were 2.71,2.81,2.73 and 2.75,respectively,which indicated that there was consistent dyeing effect between them.The results of % Tail DNA were stable with in 48 hours,and there was no significant difference between the 4 fluoresent dyes (P > O.05).The inter-assay coefficients of variation (CVs) of SYBR Green Ⅰ,Gold View and AO were 6.92%,7.10% and 8.25%,respectively,which were superior to that of EB (8.35%).The intra-assay CVs of SYBR Green Ⅰ and Gold View were 3.07% and 2.74%,respectively,which were superior to that of EB (3.59%).Conclusion SYBR Green Ⅰ,Gold View and AO may be used for the nucleic acid fluorescent staining,and especially Gold View is more suitable for instead of EB in SCGE.%目的 探讨4种核酸染料(EB、SYBR Green Ⅰ、Gold View和AO)在单细胞凝胶电泳(SCGE)中的染色特性,以分析用新型核酸荧光染料代替EB染料的可能性.方法 分离提取健康人外周血淋巴细胞,分别用0、20、40、60、80 μg/mL的H2O2进行处理,中性SCGE检测淋巴细胞DNA损伤情况;分别用EB、SYBR Green Ⅰ、Gold View和AO进行染色,荧光显微镜下观察并比较染色结果.分析尾部DNA百分含量(%Tail DNA)以比较组间差异.结果 SCGE结果表明,SYBR Green Ⅰ、Gold View和AO均能很好的反应实验结果,不同浓度中,1×~5×SYBR Green Ⅰ、2×~5 ×Gold View、2~5 μg/mL AO为最佳染色浓度范围.EB、SYBRGreen Ⅰ、Gold View和AO的回归系数分别为2.71、2.81、2.73、2.75,其染色效果较为一致,稳定性分析结果表明,3组细胞在48 h以内结果稳定,% Tail DNA差异无统计学意义(P均>0.05);SYBR Green Ⅰ、Gold View和AO实验室内精密度分别为6.92%、7.10%、8.25%,优于EB染色(8.35%);SYBR Green Ⅰ和Gold View重复性分别为3.07%、2.74%,优于EB染色(3.59%).结论 SYBR Green Ⅰ、Gold View和AO均可用于本实验染色,Gold View是更适合用于SCGE中替代EB的核酸荧光染料.
    • 徐雅梦; 姜晓冰; 于涛; 王海磊; 石磊
    • 摘要: [背景]季铵盐类消毒剂特别是苯扎氯铵(Benzalkonium chloride,BC)在食品工业中的广泛应用导致单核细胞增生李斯特菌(Listeria monocytogenes,Lm)对BC的敏感性下降.外排泵是介导Lm对BC耐受的主要机制.[目的]调查MdrL外排泵在Lm对BC耐受中的作用.[方法]利用同源重组技术构建mdrL基因缺失株.比较野生株EGD-e和突变株△mdrL在对BC的耐受性、亚致死浓度BC(2 μg/mL)胁迫下的生长情况以及致死浓度BC (16 μg/mL)下的存活率等方面的差异.[结果]构建mdrL基因缺失株△mdrL及回复突变株C△mdrL.mdrL的缺失不影响菌株对BC的最小抑菌浓度.生长曲线测定结果显示,与野生株相比,突变株在亚致死浓度BC作用下的生长迟滞期延长、平均最大生长率和平均最大光密度值均降低.当BC浓度为4 μg/mL时,梯度稀释后的野生株和突变株在平板上的生长显现出明显的差异.与野生株相比,突变株在致死浓度BC作用下的存活率降低2个log值.透射电镜的观察结果显示,加入BC后,突变株的细胞明显变细长.此外,野生株和突变株对溴化乙锭(Ethidium bromide,EB)的积累和外排无明显差异.[结论]MdrL外排泵介导Lm对BC的耐受,但是与Lm对EB的外排无关.
    • 李亚琴; 赵贵森; 詹飞
    • 摘要: 目的 探讨DNA-溴化乙锭形成的"咖啡环"用于DNA含量的测定.方法 将DNA与溴化乙锭混合,滴加在载玻片等载体上,蒸发后用凝胶成像系统观察、拍照,分析DNA的成环条件、环荧光强度与DNA浓度的相关性,以及"咖啡环"法评价DNA的灵敏度、适用范围等.结果 DNA-溴化乙锭溶液在载玻片、透明聚苯乙烯板等载体表面蒸发后可形成荧光环.在玻片上2μL点样时,DNA检出限可达到2ng/μL.在2ng/μL~1000ng/μL范围内,DNA浓度与环积分光密度拟合Logistic方程(R2=0.999).结论 "咖啡环"法简单、快速,设备要求低,可用于DNA含量的高效、快速测定.%Objective To explore the feasibility of "coffee ring" effect in DNA high-throughput evaluation. Methods we mixed uniformly DNA solution and EB solution, then dripped different size of droplets on 5 kinds of substrates. After evaporation, the coffee ring was observed and photographed with LG2020 gel imaging analysis system. Analyzed the correlation among ring formation conditions,ring fluorescence intensity and DNA concentration, and looked for the sensitivity and applicable range of the method. Result DNA - EB solution can form fluorescent ring after surface evaporation on the slide, transparent polystyrene plate carrier and so on. The DNA detection limit can be up to 2ng/μl. DNA concentration and loop integral optical density fitting Logistic equation(R2=0,999) within 2ng/μl~1000ng/μl. Conclusion The coffee ring method is simple, fast and low equipment requirement,which can be used for high-throughput evaluation of DNA solution.
    • 唐永华; 程建兵
    • 摘要: 目的 建立一种具有分裂相多、分散度好、带纹清晰、长度适中等特征的骨髓染色体G带制作方法.方法 在含9.6%胎牛血清的RPMI1640培养基中加入一定量的人淋巴瘤细胞系培养物,接种骨髓细胞后培养24h,然后加入终浓度为30μg/ml的溴化乙锭和0.06μg/ml的秋水仙胺作用1h.结果 通过方法改良,骨髓标本培养成功率约为传统方法的1.59倍,异常染色体检出率比传统方法提高了51%.结论 改良后的方法可以制作出个数更多、分散度更加良好、带纹更加清晰、染色体更长的中期分裂相,因而异常染色体检出率更高,诊断结果更加可靠.
    • 金磊
    • 摘要: Objective It is analyse two method for staining DNA fragments in agarosewith SYBR-Gold.Methods It used poststaining and prestaining to dye double strand DNA in agarose gel electrophoresis.The same way is used to stain DNA with EB as a control.And we analysed the results by the gel imaging system.Results We found SYBR-Gold was much more sensitive than EB to detect double strand DNA.But the effect of resolution and the mobility of DNA fragments were affiliated to the concentration of the samples in precasting SYBR-Gold in gels.And it was low accurate in measuring the size of DNA fragments through the agarose gel with adding SYBR-Gold in loading buffer.Conclusion We do not recommend that you stain the sample with SYBR-Gold before agarose gel electrophoresis.It is also not suitable to precast SYBR-Gold in gels when the concentration of samples is too high.However,the poststain with SYBR-Gold is an ideal method.%目的:检测 SYBR-Gold前染色与后染色的优缺点。方法采用前染色、后染色的方法使用 SYBR-Gold对双链DNA进行染色检测,EB染色作为参照,琼脂糖凝胶电泳为检测手段,凝胶成像系统拍照观察。结果 SYBR-Gold其前染色与后染色对于双链 DNA检测能力均强于 EB染色,但是 SYBR-Gold预混凝胶前染色其分辨效果及DNA迁移速度受到DNA浓度的影响;SYBR-Gold预混样品的前染色方式对于目的基因的判读误差较大。结论SYBR-Gold 不适合预混样品前染色,DNA样品浓度过大(大于250 ng)不适合预混凝胶前染色,SYBR-Gold后染色是理想的染色方式。
    • 吴晓邡; 闫秋; 戴小珍; 孟秋丽; 姜鹤群
    • 摘要: 目的:流行病学研究表明结直肠癌细胞线粒体DNA(mtDNA)拷贝数变异与患者预后具有显著相关性,但因缺乏相关体外细胞模型导致其细胞生物学效应及具体分子机制尚不明确.因此研究建立结直肠癌ρ0细胞株(无mtDNA)的方对该研究域具有重大意义.方法:在合有丙酮酸、尿苷及不同浓度溴化乙锭的培养基中培养大肠癌细胞株SW480、HCT 116、HCT-8,以不加溴化乙锭的细胞为对照,利用实时定量PCR技术监测不同处理组mtDNA拷贝数的变化,并观察结直肠癌ρ0细胞线粒体形态数目及细胞形态的变化.结果:SW480细胞用50 ng/mL EB处理25代后,再以100 ng/mL EB处理14代可成功构建SW480ρ 0细胞株;HCT116细胞加用EB 100 ng/mL培养32代,继续用EB 200 ng/mL培养10代,再用EB 500 ng/mL培养3代,可成功获得HCT116ρ 0细胞株;HCT-8细胞用200 ng/mL EB培养24代,可成功获得HCT-8ρ 0细胞株.同时,ρ0细胞较亲本细胞变大变长,其线粒体的个数增多,线粒体形态变大变长.结论:利用EB处理法可成功构建大肠癌ρ0细胞株,但不同肠癌细胞方法不尽相同.mtDNA拷贝数的降低可显著影响大肠癌细胞的形态.
  • 查看更多

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号