摘要：
本研究采用酶解法从罗非鱼皮中提取胶原蛋白肽,并对其基本成分、分子量分布、抗氧化活性、保湿性以及紫外吸收性能进行考察和分析,同时将提取到的罗非鱼皮肽与羧甲基壳聚糖按不同比例进行复配,应用到润肤霜中并进行性能评价.结果表明:酶解法所提取的罗非鱼皮肽蛋白质含量为94.75％,罗非鱼皮肽中分子量小于1000 Da的组分高达82.27％,平均分子量为614 Da,对OH-、DPPH·、O2·、清除率的IC50值分别为5.34、5.03、7.63 mg/mL,且具有良好的保湿效果,并对UBC和UVB波段的紫外线有吸收;所制备的润肤霜具有良好的保水和体外抗氧化活性以及防晒功效,其感官和理化指标符合国家润肤霜产品质量标准规定,其中RFS-4的综合效果最佳.通过裸鼠在体皮肤刺激性实验和抗皮肤光老化实验,对比不同组别裸鼠皮肤表征情况,结果表明:自制润肤霜安全无刺激并具有较好的抗皮肤光老化作用.本研究为罗非鱼皮肽在抗光老化日化产品的开发利用提供科学的理论基础.%In this experiment,enzymolysis method was employed to extract collagen peptide from the skin of tilapia.Meanwhile,its fundamental components,molecular weight distribution,anti-oxidation activity,moisture retention and UV absorption properties were also investigated and analyzed.And then the tilapia peptide and carboxymethyl chitosan,which were matched according to different proportions,were applied to prepare moisturizing cream,the properties of which were then evaluated.The result indicated that the content of tilapia skin peptide protein was 94.75％,and the molecular weight of tilapia skin peptide was less than 1000 Da(82.27％)as well as the average molecular weight was 614 Da.The IC50 values of this peptide on · OH-,DPPH· and O2-· radical scavenging activities were 5.34,5.03 and 7.63 mg/mL,respectively.Moreover,the peptide possessed favourable moisturizing efficacy,as well as UVC and UVB absorbing performance to some extent.The results also revealed that the prepared skin care cream,mainly including the above-mentioned peptide and carboxymethyl chitosan,had good moisturizing and anti-aging efficacy,as well as sun-block effect.Besides,the sensory and physicochemical property of this cream conformed to the regulation of national moisturizing cream product quality standards.Furthermore,skin irritation and skin anti-photoaging tests were carried out using the nude mice,and the results suggested that this homemade moisturizer was safe and nonirritating,and had prevented skin photoaging efficacy on the basis of comparison between different groups.This study provided a scientific theoretical basis for the development and utilization of tilapia skin peptides in anti-aging products.
