流式细胞仪分析

摘要： BackgroundThe 导致了 pluripotent 干细胞(iPSC ) 在心肌的梗塞(MI ) 的细胞的治疗显示出大潜力，当它的申请被 cardiomyocyte 区别的低效率妨碍时。现在的学习被设计从老鼠在 cardiomyocyte 区别上调查 cardiotrophin-1 ( CT-1 )的效果导致的 pluripotent 干细胞( miPSCs )和内在的机制 involved.MethodsThe 为从 miPSCs 的 cardiomyocyte 区别的最佳的治疗状况与理想的集中( 10 ng/mL )和持续时间被建立(从到白天的白天 3 14 ) CT-1 管理。说明了胚胎的 cardiogenesis 的心脏的特定的基因的起来调整的表示被量的 RT-PCR 观察。 α 的提高的数量；肌浆球蛋白重链( α ；-MHC)和心脏的 troponin 我( cTn 我)积极房间被 immunofluorescence 在显微镜的分析揭示了的 CT-1 group.ResultsTransmission 电子染色和流动 cytometry 分析检测与 CT-1 对待的房间更好出现了，这组织了 sacromeric 结构和更多线粒体，它是成熟 cardiomyocytes 的词法特征。当与控制 group.ConclusionsThese 相比调查结果建议 CT-1 能提高 cardiomyocyte 区别以及导致的 pluripotent 干细胞导出的老鼠的成熟，西方的污点证明 CT-1 经由 JAK2/STAT3/Pim-1 小径部分从 miPSCs 支持 cardiomyocyte 区别由调整 JAK2/STAT3/Pim-1signaling 的 cardiomyocytes 小径。

摘要： Objective: The aim of this study was to investigate the impact of beta-elemene injection on the growth and beta-tubulin of human hepatocarcinoma HepG2 cells. Methods: Cell proliferation was assessed by MTT assay. Cell cycle distribution was detected by flow cytometry(FCM). The mRNA expression of beta-tubulin was measured by RT-PCR. Western blot analysis was used to determine protein expression of beta-tubulin and the polymerization of beta-tubulin. Results: Beta-elemene injection inhibited HepG2 cells proliferation in a dose- and time-dependent manner; FCM analysis indicated beta-elemene injection induced cell cycle arrested at S phase. RT-PCR and western-blot analysis showed that beta-elemene injection down-regulated beta-tubulin expression at both mRNA and protein levels, presenting a dose-dependent manner. Moreover, beta-elemene injection reduced the polymerization of microtubules in a dose-dependent manner. Conclusion: Beta-elemene injection can inhibit the proliferation of hepatoma HepG2 cells, the mechanism might be partly related to the down-regulation of beta-tubulin and inhibition of microtubular polymerization.

摘要： Objective: The purpose of the study was to study the effect of Huaier, a traditional Chinese medicine, on the cell cycle adjustment in MOLT4 cells in vitro. Methods: We used MTT assay to test cell viability, flow cytometry to detect cell cycle and apoptosis and western blot to examine the expression of cell-cycle and apoptotic proteins in MOLT4 cells induced by Huaier. Results: Huaier could reduce the viability of MOLT4 cell by inducing G1 arrest and apoptosis. The induction of apoptosis after treatment with Huaier for 24 h was demonstrated in a dose- and time-dependent manner by flow cytometry analysis. G1 arrest induced by Huaier was modulated through the increased expression of Cdki proteins(p21cip/waf1 and p27kip1) with a simultaneous decrease in Cdk2, Cdk4, Cdk6, cyclin D1 and cyclin E expression. Huaier also induced Bax and Bcl-2 expression and activation of Caspase-3. Conclusion: It is firstly demonstrated that Huaier can inhibit proliferation of MOLT4 cells via G1 arrest and apoptosis. These results suggest that Huaier is a cell-cycle anti-cancer drug.

摘要： Objective: The aim of the study was to investigate the effect of SGI-1776 combination with DDP in sub-toxic concentration on induction of apoptosis of human ovarian cancer HO-8910 cells in vitro and to unravel the associated mechanisms. Methods: Human ovarian cancer HO-8910 cells were cultured in vitro. The inhibitory effect of SGI-1776 combination with DDP in sub-toxic concentration on induction on viability of human ovarian cancer HO-8910 cells was evaluated by the MTT assay. Cell apoptosis rate was analyzed by flow cytometry. The proteins expression level related to apoptosis were analyzed by Western blot. Results: SGI-1776 combination with DDP in sub-toxic concentration significantly inhibited the proliferation of human ovarian cancer HO-8910 cells, and proliferation inhibition rate was increased drastically compared with normal saline(NS) group or DDP group in sub-toxic concentration or SGI-1776 group in sub-toxic concentration(P﹤0.01). Apoptosis rate markedly increased after the treatment of SGI-1776 combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of Bax and Cyto-c were depressed by SGI-1776 combination with DDP in sub-toxic concentration. Conclusion: SGI-1776 combination with DDP in sub-toxic concentration could inhibit the cell proliferation and lead to cell apoptosis inhuman ovarian cancer HO-8910 cells, and its mechanism may be related to through mitochondrial apoptotic pathway.

摘要： 目的：研究右旋娃儿藤宁碱类化合物（CAT1、CAT3和CAT5）在体内外对神经胶质瘤生长的抑制作用及其作用机制。方法：体外噻唑蓝比色法（MTT）和克隆原形成实验观察不同浓度不同时间作用下CAT1对人胶质瘤细胞SHSY-5Y和U251细胞株增殖能力的影响；流式细胞仪分析CAT1作用于SHSY-5Y和U251细胞72h后细胞凋亡状况及细胞周期分布变化；

摘要： Objective: The aim of the study was to investigate the apoptosis induced by piperlongumine on human breast adenoma MDA-MB-231 cells and the mechanism involved. Methods: Human breast adenoma MDA-MB-231 cells line was cultured in vitro. The inhibitory effect of piperlongumine on the proliferation of human breast adenoma MDA-MB-231 cells was measured by CCK-8 assay. Distribution of cell cycle was analyzed by flow cytometry. The apoptosis rates of MDA-MB-231 cells were measured using Annexin V/PI staining. The flow cytometry with the probe of DCFH-DA was used to detect the intracellular reactive oxygen species levels. Western blot was used to explore the protein expression of Bcl-2 and Bax. Results: The CCK-8 assay showed that piperlongumine had an inhibiting effect on the proliferation of MDA-MB-231 cells in a concentrationand time-dependent manner. MDA-MB-231 cells were markedly arrested at G0/G1 phase after treatment of piperlongumine. Piperlongumine induced apoptosis of MDA-MB-231 cells obviously. The level of intracellular reactive oxygen species was increased in a dose-dependent manner. The antioxidant N-acetyl-L-cystein inhibited the apoptosis of cells and the level of intracellular reactive oxygen species was also decreased. By Western blot analysis, we found the expression of Bax was up-regulated whereas that of Bcl-2 was down-regulated in a concentration-dependent manner. Conclusion: Piper-longumine possesses a significant function for inhibiting proliferation, arresting cells at G0/G1 phase and inducing apoptosis of MDA-MB-231 cells, which seems to be associated with the increased generation of intracellular reactive oxygen species as well as the down-regulation of Bcl-2 and up-regulation of Bax.

摘要： OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer.

摘要： 棉花组织和细胞培养工作受到国内外广泛重视.体细胞胚胎发生(somatic embryogenesis)是体细胞向胚胎发生途径转变的发育再建(developmental reprogramming)过程,是植物发育过程中的独特现象.体细胞胚胎发生现象被看作是开展生物学理论研究的良好模型.研究棉花体细胞胚胎发生过程中基因表达的转录谱,鉴定并最终克隆体细胞胚胎发生相关的关键基因,分析该生物过程的细胞学和内源生化因子动态变化,对阐明植物界体细胞胚发生和发育的机理,促进棉花的遗传工程改良,在基础理论和实际应用价值上都有重要意义.

摘要： 棉花组织和细胞培养工作受到国内外广泛重视.体细胞胚胎发生(somatic embryogenesis)是体细胞向胚胎发生途径转变的发育再建(developmental reprogramming)过程,是植物发育过程中的独特现象.体细胞胚胎发生现象被看作是开展生物学理论研究的良好模型.研究棉花体细胞胚胎发生过程中基因表达的转录谱,鉴定并最终克隆体细胞胚胎发生相关的关键基因,分析该生物过程的细胞学和内源生化因子动态变化,对阐明植物界体细胞胚发生和发育的机理,促进棉花的遗传工程改良,在基础理论和实际应用价值上都有重要意义.

摘要： 棉花组织和细胞培养工作受到国内外广泛重视.体细胞胚胎发生(somatic embryogenesis)是体细胞向胚胎发生途径转变的发育再建(developmental reprogramming)过程,是植物发育过程中的独特现象.体细胞胚胎发生现象被看作是开展生物学理论研究的良好模型.研究棉花体细胞胚胎发生过程中基因表达的转录谱,鉴定并最终克隆体细胞胚胎发生相关的关键基因,分析该生物过程的细胞学和内源生化因子动态变化,对阐明植物界体细胞胚发生和发育的机理,促进棉花的遗传工程改良,在基础理论和实际应用价值上都有重要意义.

摘要： 棉花组织和细胞培养工作受到国内外广泛重视.体细胞胚胎发生(somatic embryogenesis)是体细胞向胚胎发生途径转变的发育再建(developmental reprogramming)过程,是植物发育过程中的独特现象.体细胞胚胎发生现象被看作是开展生物学理论研究的良好模型.研究棉花体细胞胚胎发生过程中基因表达的转录谱,鉴定并最终克隆体细胞胚胎发生相关的关键基因,分析该生物过程的细胞学和内源生化因子动态变化,对阐明植物界体细胞胚发生和发育的机理,促进棉花的遗传工程改良,在基础理论和实际应用价值上都有重要意义.

摘要： 棉花组织和细胞培养工作受到国内外广泛重视.体细胞胚胎发生(somatic embryogenesis)是体细胞向胚胎发生途径转变的发育再建(developmental reprogramming)过程,是植物发育过程中的独特现象.体细胞胚胎发生现象被看作是开展生物学理论研究的良好模型.研究棉花体细胞胚胎发生过程中基因表达的转录谱,鉴定并最终克隆体细胞胚胎发生相关的关键基因,分析该生物过程的细胞学和内源生化因子动态变化,对阐明植物界体细胞胚发生和发育的机理,促进棉花的遗传工程改良,在基础理论和实际应用价值上都有重要意义.

摘要： 本发明涉及用于磁流式细胞仪的盒（1），其主要在x‑y‑平面中延伸，其具有用于将样本（15）注射到盒（1）中的入口（2）、用于带有磁性标记以标记样本（15）中的预先给定颗粒（16、16'）的缓冲溶液（21）的泡形罩（3）、出口、及流体通道（9），流体通道（9）包括连接入口（2）与泡形罩（3）的第一部分和连接第一部分与出口的第二部分，其中，流体通道（9）的第二部分包括富集区（5）和在富集区（5）和出口之间的测量区（6），富集区（5）带有机械引导结构，以将样本（15）中的已标记颗粒（16、16'）聚集在流体通道（9）的预定分区中，测量区（6）包括在流体通道（9）的预定分区中的磁场传感器（14），以便提供用于测量样本中的颗粒，尤其是颗粒的浓度的简化且加速的器件。

摘要： 一种光学引擎，用于台式流式细胞仪，该光学引擎具有一组激光器，每个激光器沿x轴水平聚焦到相同的水平位置，沿流动池的相同激发平面沿y轴垂直聚焦到不同的垂直位置，一组光学器件，根据激发荧光的不同激光，将相同波长范围的荧光分离到收集光学器件的焦平面中的不同位置；以及检测器，选择性地从不同的位置检测光，从而根据不同的激光器激发在相同波长范围内发射的荧光之间的区分。

摘要： 本申请公开了用于台式流式细胞仪的光学引擎，该光学引擎包括激光源组；用于每个激光源的不同的光束整形光学系统组，其中每一组包括两个透镜，沿X轴水平可调地聚焦光线到同一水平位置，并且沿Y轴竖直可调地聚焦光线到沿同一平面的不同竖直位置；采集光学系统，其从流通室中采集荧光；过滤光学系统，其根据波长范围将从流通室中已采集的荧光过滤到不同检测通道中；以及用于每个检测通道的检测器，其将过滤的荧光转换成电信号，其中电信号被处理以使得来自位于不同竖直位置的每个激光源的荧光在同一检测器中被区分。

摘要： 本发明涉及用于磁流式细胞仪的盒（1），其主要在x‑y‑平面中延伸，其具有用于将样本（15）注射到盒（1）中的入口（2）、用于带有磁性标记以标记样本（15）中的预先给定颗粒（16、16'）的缓冲溶液（21）的泡形罩（3）、出口、及流体通道（9），流体通道（9）包括连接入口（2）与泡形罩（3）的第一部分和连接第一部分与出口的第二部分，其中，流体通道（9）的第二部分包括富集区（5）和在富集区（5）和出口之间的测量区（6），富集区（5）带有机械引导结构，以将样本（15）中的已标记颗粒（16、16'）聚集在流体通道（9）的预定分区中，测量区（6）包括在流体通道（9）的预定分区中的磁场传感器（14），以便提供用于测量样本中的颗粒，尤其是颗粒的浓度的简化且加速的器件。

摘要： 本发明提供一种流式细胞仪液路系统，包括：流动室；样本液供给模块，其包括进样针、第一柱塞泵、第一三通阀、样本液供给管路；第一三通阀进口端与进样针通过样本液供给管路相连，其两个出口端通过样本液供给管路分别与流动室、第一柱塞泵相连；鞘液供给模块，其包括鞘液桶、第二三通阀、定量泵、滤芯、加热装置、鞘液供给管路；定量泵分别与鞘液桶、滤芯顶部相连；加热装置分别与滤芯底部、流动室相连；第二三通阀设置于定量泵与鞘液桶相连的鞘液供给管路上，用以在鞘液供给过程中在滤芯顶部存储空气；取样模块；清洗模块。本发明还提供一种流式细胞仪。通过改进样本液供给模块及鞘液供给模块的液路结构设计，以提高样本液及鞘液流动的稳定性。

摘要： 一种流式细胞仪的激光照射系统及采用这种激光照射系统的流式细胞仪，该激光照射系统的各激光照射机构的传播光路从不同方向独立地延伸向流动室，并且通过各自的光学组件来保证各路激光照射机构照射到流动室的聚焦光斑的大小大致相同。因为该系统不需要将多路激光光路合并到同一光路上，因此可省略二向色镜的使用，同时也节省了相应的结构布置，从而使该激光照射系统体积更小，更利于流式细胞仪的小型化设计。

摘要： 本发明提供一种基于多激光流式细胞仪的数据处理方法，包括步骤：阈值设定、通道触发、延迟数据处理。本发明还涉及基于多激光流式细胞仪的数据处理系统及流式细胞仪控制系统。本发明通过一个激光器对应一个数据处理子板，可以更加灵活的进行系统配置，不同数量的激光，不同数量的采集通道，以及不同模块的升级、增减。另外，本发明根据激光器之间的延迟，为每个数据处理子板开辟特定大小的数据缓冲区，来存放第一个激光器和最后一个激光器延迟的这段时间所采集的数据，以便于任意选定触发通道。

摘要： 本申请公开了一种用于流式细胞仪的侧向光束收集方法、装置以及流式细胞仪，其中用于流式细胞仪的侧向光束收集装置包括：光束干涉组件，光束干涉组件用于接收流式细胞仪的侧向光束，并使侧向光束发生干涉形成干涉图数据，而后将干涉图数据发送给流式细胞仪的信号处理装置，通过该侧向光束收集装置，能够简化流式细胞仪。

摘要： 本发明公开了一种流式细胞仪的液流系统、气密性检测方法与流式细胞仪。该液流系统包括鞘液装置、负压装置、测试装置以及上样装置。鞘液装置包括连通进液口的鞘液管道、设在冲罐管道上的第一鞘液控制阀以及设在鞘液管道上的鞘液泵、鞘液压力传感器、第二鞘液控制阀，冲罐管道还连通鞘液管道；负压装置连通流动室以用于对液流系统产生负压；测试装置包括流动室以及设在流动室的出液口处的负压控制阀；上样装置包括连通流动室的上样口的上样管道、设在上样管道上的采样部件和样本控制阀。该液流系统能够提高液路气密性检测和堵塞检测准确性且能够降低仪器成本。

摘要： 一种流式细胞仪的激光照射系统及采用这种激光照射系统的流式细胞仪，该激光照射系统的滤光片相对光轴倾斜设置，光电元件对应设置在滤光片的反射光路上。该倾斜放置的滤光片在滤除杂散光的同时，还反射一部分光到光电二极管上，用于LD功率检测。因此，可以节省原有的反射用玻璃板，使激光照射系统更加紧凑，结构也更加简单，不仅成本更低，组装也更加方便。